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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Immunol. | doi: 10.3389/fimmu.2019.02527

Serum clusterin and complement factor H may be biomarkers differentiate primary Sjögren's syndrome with and without neuromyelitis optica spectrum disorder

 Lin Qiao1,  Chuiwen Deng1, Qian Wang1, Wen Zhang1, Yunyun Fei1, Yan Xu1, Yan Zhao1* and Yongzhe Li1*
  • 1Peking Union Medical College Hospital (CAMS), China

Background Neuromyelitis optica spectrum disorder (NMOSD) is a neurological complication of primary Sjögren's syndrome (pSS).
Objective We aimed to explore potential serological differences between pSS patients with and without NMOSD.
Methods There were 4 pSS patients with NMOSD and 8 pSS patients without NMOSD enrolled as the screening group for two-dimensional difference gel electrophoresis (DIGE) analysis. Then differential expressed protein spots between groups were identified by MALDI-TOF/TOF MS. The levels of the identified potential biomarkers were verified by ELISA in a second independent cohort including 22 pSS patients with NMOSD, 26 pSS without NMOSD and 30 NMOSD patients.
Results Nine proteins were identified significantly differently expressed (more than 1.5-fold, p<0.05) between these two groups. Serum levels of clusterin and complement factor H (CFH) were further verified by ELISA. Results showed that the serum clusterin was significantly higher in NMOSD with pSS than without (298.33±184.52ng/ml vs. 173.49±63.03ng/ml, p<0.01), while the levels of CFH were lower in pSS patients with NMOSD than without (24.19±1.79 ng/ml vs. 25.87±3.98ng/ml, p<0.01).
Conclusion This is the first study of serological comparative proteomics between pSS patients with and without NMOSD. Serum clusterin and CFH might be potential biomarkers for pSS patients with NMOSD and play important role in the pathogenesis of the disease but needs further verification.

Keywords: primary Sjögren's syndrome, Proteome, Neuromyelitis optica spectrum disorder, Clusterin, Complement Factor H

Received: 13 Aug 2018; Accepted: 10 Oct 2019.

Copyright: © 2019 Qiao, Deng, Wang, Zhang, Fei, Xu, Zhao and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Yan Zhao, Peking Union Medical College Hospital (CAMS), Beijing, 100730, Beijing Municipality, China, zhaoyan_pumch2002@aliyun.com
Prof. Yongzhe Li, Peking Union Medical College Hospital (CAMS), Beijing, 100730, Beijing Municipality, China, yongzhelipumch@126.com