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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Immunol. | doi: 10.3389/fimmu.2019.02708

TcellSubC: an atlas of the subcellular proteome of human T cells

 Rubin N. Joshi1*, Charlotte Stadler2,  Robert Lehmann3, Janne Lehtio1, 2,  Jesper Tegner1, 3,  Angelika Schmidt1, 4 and  Mattias Vesterlund1, 2*
  • 1Karolinska Institute (KI), Sweden
  • 2Science for Life Laboratory (SciLifeLab), Sweden
  • 3King Abdullah University of Science and Technology, Saudi Arabia
  • 4Institute for Immunology, Biomedical Center, Ludwig Maximilian University of Munich, Germany

We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 minutes and 1 hour of T cell receptor (TCR) stimulation respectively. We quantified the subcellular distribution of 6572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

Keywords: Proteomics, protein translocation, TCR stimulation, T cells, Subcellular localization, subcellular fractionation, mass spectrometry (MS)

Received: 04 Jul 2019; Accepted: 04 Nov 2019.

Copyright: © 2019 Joshi, Stadler, Lehmann, Lehtio, Tegner, Schmidt and Vesterlund. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Rubin N. Joshi, Karolinska Institute (KI), Solna, Sweden, rubinjoshi11@gmail.com
Dr. Mattias Vesterlund, Karolinska Institute (KI), Solna, Sweden, mattias.vesterlund@ki.se