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Case Report ARTICLE

Front. Immunol., 20 March 2020 | https://doi.org/10.3389/fimmu.2020.00415

Intact B-Cell Signaling and Function With Host B-Cells 47 Years After Transplantation for X-SCID

  • Division of Immunology, Allergy, and Rheumatology, Department of Pediatrics, University of California, Los Angeles, Los Angeles, CA, United States

Introduction: Severe Combined Immunodeficiency (SCID) is a life-threatening immunodeficiency caused by several pathogenic genetic variants, and it is characterized by profound defects in T-cell numbers and immune function. First performed in the late 1960's, hematopoietic stem cell transplantation remains the standard treatment for most cases of SCID. There is a growing number of post-transplant SCID patients, and it is imperative to assess the long-term outcomes of these patients. We have reported here the longest follow-up of a post-transplant SCID patient who, to our knowledge, bears the first gamma chain (γc) variant to show intact IL-21 signaling.

Case Presentation: The patient presented at 5 months of age with recurrent thrush and Pneumocystis jiroveci pneumonia. In 1971, at the age of 11 months, he received an unconditioned, matched, related donor transplant comprising whole, unprocessed bone marrow. He is now 48 years old without significant illness and has never required immunoglobulin replacement. He exhibits T-dependent vaccine responses. He does suffer from chronic warts and bacterial infections that have worsened in recent years. We confirmed a known pathogenic variant in the IL2RG gene showing a hemizygous variant NM_000206.2:c.675C>A, resulting in p.Ser225Arg. His chimerism studies revealed donor T cells, host B cells, host myeloid cells, and mixed NK cells. Lymphocyte enumeration revealed normal numbers and distribution of B cells. The host B cells carry the pathogenic variant in IL2RG, but, when stimulated with IL-21, they demonstrated intact, γc-dependent signaling.

Conclusions: Even with host B cells, reconstitution with donor T cells can be sufficient to allow over four decades of survival when B-cell function is intact. Our case demonstrates that satisfactory B-cell function can arise as a consequence of both intact IL-21 signaling due to a hypomorphic γc variant, and close HLA matching with the donor to allow for effective T-cell help.

Introduction

Severe Combined Immunodeficiency (SCID) is a collection of life-threatening diseases characterized by an absence of functional T cells and requiring treatment with hematopoietic stem cell transplantation (HSCT), gene therapy, or enzyme replacement therapy. At least 70 SCID patients born yearly in the United States and Canada undergo definitive treatment (1, 2). Over the past 10 years, the addition of T-cell receptor excision circle (TREC) assays to the newborn screen has allowed for earlier diagnosis, therapy, and improved survival for patients with SCID (3). With a growing population of post-transplant SCID patients living into adulthood with a wide spectrum of immune functions, it is critical to understand their long-term outcome.

The most common genetic defects resulting in SCID are IL-2 receptor gamma (IL2RG) gene variants causing X-linked SCID (X-SCID) (2). This gene encodes the common gamma chain (γc), which is a subunit of the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptors. X-SCID is typically characterized by the absence of T and NK cells due to ineffective signaling of IL-7 and IL-15, respectively (4). B cells in X-SCID are typically normal in number, but they have an abnormal function due to impaired IL-21 signaling, which is critical for the maturation, differentiation, and activation of the B-cell lineage (4, 5).

The survival rate of patients with SCID who receive HLA-identical transplants is ~90% (3, 6). Long-term survival is affected by the causal genetic defect, donor match, conditioning regimen, age at transplant, and clinical status of the patient prior to transplant. The single most important factor in survival is donor match, with HLA-identical sibling donors providing the best outcomes (3, 7). The majority of deaths result from infections at the time of transplant or within the first year post-transplant, with the second leading causes of death being pulmonary disease or acute respiratory distress syndrome (3, 7, 8). However, sequalae for long-term survivors have not been systematically reported.

Case Presentation

The patient is a 48-year-old man with a family history that includes the infantile deaths of three maternal uncles. He presented at 5 months of age with recurrent oral thrush and Pneumocystis jiroveci pneumonia. He later developed Candida esophagitis and failure to thrive. The patient received an unconditioned transplant at 11 months of age in 1971 that comprised whole, unprocessed bone marrow (9). HLA compatibility with his 11-year-old sister, the donor, was determined by HLA serotyping and mixed leukocyte culture. The immediate post-transplant course was complicated by acute Graft vs. Host Disease (GvHD) with rash, fever, hepatosplenomegaly, and respiratory distress that resolved after treatment with prednisone. In the first 10 years post-transplant, he developed chronic dry eyes, a few cases of pneumonia, and one episode of cellulitis. He was lost to follow-up at age 17.

He was referred back to UCLA Immunology three decades later because of the failure of four corneal transplants. The patient reported living a “normal life” working as a plumber. He had never required immunoglobulin (Ig) replacement or nutritional support. He had recurrent dental infections, and one skin carcinoma had been removed. He developed cutaneous, oropharyngeal, and genital warts that relapsed and remitted but had worsened in recent years (Figure 1A). In the prior 5 years, he had three pneumonias, chronic sinusitis (Figure 1B), and a skin infection due to MRSA.

FIGURE 1
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Figure 1. (A) Numerous, confluent cutaneous warts on palmar surface of hand. (B) CT sinuses showing turbinate edema and maxillary sinus mucosal thickening with layering fluid.

The IL2RG gene was sequenced from patient's buccal mucosal cells, and this revealed a hemizygous variant on Chromosome X at location g.70329160 (based on build GRCh37/hg19), NM_000206.2:c.675C>A, resulting in p.Ser225Arg. Cases of X-SCID have been reported due to missense variants of nearby amino acids (R222C, R224W, R226C/H, F227C, and L230P) (10). This variant does not appear in large population databases of healthy subjects (11) and has been identified as pathogenic in one other infant with a TB+NK SCID phenotype (12). Thus, we diagnosed our patient with X-SCID.

Lymphocyte enumeration showed normal numbers of T and B cells but low numbers of NK cells. A predominantly memory phenotype was seen for both CD4+ and CD8+ T cells (Table 1). TRECs were absent. Lymphocytes responded with modest proliferation after stimulation with mitogens PHA, PWM, and ConA. There was a minimal proliferative response to either tetanus antigen or Candida. The response to Staph enterotoxin B stimulation was robust. Foxp3+ Tregs comprised 2.7% of circulating CD4+ T cells (normal).

TABLE 1
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Table 1. Laboratory results.

The diversity of the patients' T cells was examined by assessing the frequency of usage of 24 TCR Vβ regions in T cells by flow cytometry. We found 31.1% of his T cells were Vβ3+ (normal range 0.6–8.2%), 22.5% of his T cells were Vβ1+ (normal range 1.9–4.7%), and 5.7% of his T cells were Vβ16+ (normal range 0.5–2.9%), while proportions of 15 other Vβ regions were lower than normal. Thus, while T-cell numbers were normal, the patient's T cells had a diminished repertoire.

In vitro assays revealed that NK-cell-dependent cytotoxicity was impaired in the patient. We examined killing after normalizing for numbers of NK cells and offered a range of erythroblastoid target cell concentrations, but killing was <2% and was not accentuated by pre-incubation with the γc cytokines IL-2 or IL-15. This result suggested that his NK cells were poorly functional.

HLA typing was performed by sequencing DNA isolates from the patient's buccal mucosal cells, confirming a 12-out-of-12 match with the donor. To assess engraftment and the origins of specific hematopoietic lineages, we performed chimerism studies on sorted cells by short-tandem repeats. As expected, myeloid cells were entirely from the recipient, and T cells were entirely from the donor. However, B cells were 93% from the recipient and 7% from the donor, and the degree of uncertainty in this test did not preclude the possibility that 100% of the B cells are from the recipient. NK cells were 69% from recipient. To further understand the origins of these cells, we rigorously purified T cells, B cells, and monocytes by flow sorting and analyzed each population with whole exome sequencing. None of the T cells showed rare variants in IL2RG, while 100% of the monocytes and B cells showed the pathogenic variant of IL2RG. Thus, a somatic reversion mutation was not responsible for the resiliency of the patients' B cells nor were any of the B cells derived from the donor.

Thirty percent of the patient's B cells had a memory phenotype, and 5% were of the switched memory subtype (Table 1). These proportions were normal for his age. His serum immunoglobulin levels were normal to elevated across all isotypes, and he had never been on Ig replacement. B-cell function, as measured by antibody titers, showed measurable titers to tetanus, CMV, and HSV-1 but no protective titers for Streptococcus pneumoniae, polio, or HiB. He had absent titers to EBV and HSV-2. Boosting with polysaccharide pneumococcal vaccine resulted in poor responses (Table 1). These studies suggest a specific antibody defect to polysaccharide antigens but otherwise relatively intact B-cell function.

To better delineate our patient's B-cell function, we studied the response of his lymphocytes to cytokines that signal through the γc receptor (Figure 2). Typically, post-HSCT X-SCID patients with split chimerism show low-to-normal in vitro B-cell responses to stimulation with IL-4 but low-to-absent responses to IL-21 (13). This discrepancy is attributed to the ability of IL-4 to bind and signal through either a receptor comprising γc and IL-4RA or an alternate receptor comprising IL-4RA and IL-13R chains. On the other hand, γc is indispensable for IL-21 signaling (5, 13, 15). We found our patient's T cells showed normal γc signaling (Figure 2A). When we tested B-cell responses to γc-dependent cytokines, we found that B cells stimulated with IL-21 showed normal phosphorylation of STAT3 (Figure 2B). This result suggests that the genomic variant γc, which is present in the B cell population, retains normal IL-21 signaling. Most NK cells showed normal γc signaling (Figure 2C), corresponding to the degree of chimerism. Finally, we found that IL-4 stimulation showed normal phosphorylation of STAT6 in B cells and monocytes.

FIGURE 2
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Figure 2. (A) IL-2, IL-4, and IL-21 stimulation in CD4+ T cells in control, sister (donor) and patient, as measured by pSTAT5, pSTAT6, and pSTAT3, respectively. (B) IL-4 and IL-21 stimulation of B cells in control, sister (donor), and patient as measured by pSTAT6 and pSTAT3. (C) IL-2 and IL-15 stimulation in NK cells in control and patient as measured by pSTAT5. (D) IL-4 stimulation in CD14+ monocytes in control and patient as measured by pSTAT6.

Discussion

To our knowledge, this is the longest follow-up of a SCID patient who received an allogeneic transplant. The first SCID transplants were performed in the late 1960s (16, 17), and the few patients who received transplants prior to ours, to the best of our knowledge, have not had immune evaluations beyond three decades post-transplantation (17, 18). Our patient had experienced some complications post-transplant, including acute GvHD, bacterial infections (pneumonia, sinusitis, and cutaneous abscesses), and chronic warts. However, he had not experienced other common complications of post-transplant SCID patients, such as chronic GvHD, severe or opportunistic infections, or autoimmunity, as seen in up to 15% of cases. More importantly, he has not required Ig replacement or nutritional support as required in a subset of post-transplant patients (7, 14, 18, 19).

Warts due to HPV infection typically develop 10 years after a transplant and continue to worsen over time. Warts are especially common post-transplantation in patients bearing pathogenic variants in IL2RG and JAK3 with as many as 26% of X-SCID patients developing warts (8, 18, 20). Defective γc signaling in keratinocytes, particularly through the IL-15 receptor, may also contribute to wart susceptibility by reducing chemokine production and CD4+ T cell recruitment (21). As in our patient, low NK cell counts are found in up to 33% of post-transplant patients, and may further contribute to HPV susceptibility (22). Defects in NK cell function also increase risk of viral induced cancers (23, 24). HPV infection, including genital warts, and poor NK killing in our patient put him at high risk for squamous cell carcinoma, highlighting the need for continued monitoring.

Many SCID patients require Ig replacement after transplantation, particularly in X-SCID patients who underwent transplantation without conditioning (8, 14). Whether B-cell chimerism influences B-cell function and the need for Ig replacement is controversial (7, 13, 25). As was the case in our patient, a few post-transplant X-SCID patients without B-cell chimerism have not required Ig replacement and even demonstrated antibody responses to neoantigens such as φX174 (13). The mechanism for adequate host B-cell function in these X-SCID patients is not understood (13).

Long-lived protective antibody responses and the formation of memory B cells are dependent on IL-21 signaling through a normal γc (5, 15, 26). In post-transplant patients, at least 10% of B cells must have adequate IL-21 signaling for B cells to function (5, 15). Our patient's B cells were host-derived and carried the pathogenic genomic variant. Yet, IL-21 signaling was functionally intact, as demonstrated in vitro by the normal phosphorylation of STAT3 in response to IL-21 and in vivo by normal numbers and proportions of memory B cells, normal levels of all immunoglobulins, and relatively intact antibody responses. We hypothesize that there may be leaky IL-21 function in post-transplant patients who maintain adequate B cell function but lack donor B cells, allowing them to meet this critical 10% functional threshold.

Normal signaling through the IL-21 receptor in B cells bearing a pathogenic γc is novel to our knowledge. However, it is well-known that missense variants in γc can lead to atypical SCID (2). Missense variants in exon 5, where our patient's variant falls, account for 29.4% of known γc mutations. Phenotypes in this region are highly variable based on the variant. If exon 5 variants fall in the highly conserved WSXWS motif, the effect is a severe phenotype (27), whereas other nearby variants can confer differential loss of cytokine signaling. For example, in the nearby p.R222C (4). In these patients, IL-4 signaling was comparable to control, some IL-15 and IL-2 signaling were seen at high agonist concentrations, but IL-21 signaling was absent. As a consequence, intact numbers of NK cells can often be seen in p.R222C (27), and even normal T-cell development has been noted (28). In contrast, our patient's variant resulted in an amino acid substitution 12 amino acids upstream from the WSXWS motif (29) and his B cells showed normal signaling in response to stimulation with IL-21.

Our patient has illustrated the excellent survival of matched sibling donor transplants (3). He has also demonstrated the importance of long-term follow-up, given unknown graft duration, morbidity associated with HPV infection, and concern for malignancy. Overall, survival post-transplantation has improved over time (3). However, the incidence of repeat transplants or gene therapy after initial transplant, has increased in the past 20 years due to reduced intensity conditioning regimens (7). In our patient, the natural aging of the donor T cells and lack of genesis of new T cells suggests that he may benefit from a booster transplant from his sister for his persistent cutaneous warts and recent bacterial infections.

Summary

In summary, 47 years after the unconditioned bone marrow transplant, our patient was doing relatively well. His T cells were adequate in number, although somewhat oligoclonal. Importantly, they were functionally sufficient, able to maintain adequate immunity against most pathogens, and able to provide B-cell help for T-dependent humoral responses. His ongoing B-cell function was attributable to a combination of close HLA matching to his donor, allowing for effective T-cell help, and a hypomorphic γc mutation that allowed for IL-21 signaling. We speculate that other X-SCID patients with functional host B cells post-transplant may bear similar hypomorphic mutations that allow for functionally relevant cytokine signaling.

Methods

Reagents

Several antibodies were used: anti-pStat3 (Tyr705) AF647 (clone 4/P-Stat3), anti-pStat6 (Tyr641) AF647 (clone 18/P-Stat6), anti-pStat5 (Tyr694) AF488 [clone 47/Stat5(pY694)], and Mouse Isotype Control AF488 or AF647 (clone MOPC-173) from BD Biosciences; anti-CD4 AF488 or BV421 (clone OKT4), anti-CD14 BV421 (clone HCD14), anti-CD19 BV421 (clone HIB19), and Human TruStain FcX from Biolegend. All cytokines were from Peprotech: IL-2 (Cat# 200-02), IL-4 (Cat# 200-04), and IL-21 (Cat# 200-21).

Phospho-Stat Assays

Stimulation: 20 μL of 10X cytokines in PBS (or PBS only control) were added to 180 μL of heparinized whole blood in a 5 mL FACS tube to achieve these final concentrations: IL-2 (10 ng/mL), IL-4 (100 ng/mL), or IL-21 (50 ng/mL). Tubes were incubated at 37°C for 20 min, at which point 4 mL of pre-warmed 1X BD Lyse/Fix buffer (Cat# 558049) was added. Cells were fixed for 10 min at 37 °C, centrifuged and washed twice with FACS buffer (1X PBS supplemented with 2% FBS and 1 mM EDTA). Staining and permeabilization: Fc receptors were blocked for 5 min at RT, followed by a 20 min stain on ice with anti-CD4 and anti-CD19. Cells were washed with FACS buffer and permeabilized for 30 min on ice with 1 mL BD Phosflow Perm Buffer III (Cat# 558050) that had been pre-cooled to −20°C. After permeabilization, 2 mL FACS buffer was added and the samples were centrifuged. After three additional washes, the cells were stained with anti-pStat antibodies or an isotype control for 30 min at RT. Samples were washed three times and data were collected on a Cytek DxP10 flow cytometer. Data were analyzed with FlowJo software.

Data Availability Statement

All data for this paper are available in this article/supplementary materials.

Ethics Statement

The studies involving human participants were reviewed and approved by UCLA Office of the Human Research Protection Program. The patients/participants provided their written informed consent to participate in this study. Written informed consent was obtained from the individual(s) for the publication of any potentially identifiable images or data included in this article.

Author Contributions

CD, MG-L, and MB provided care. CD wrote the manuscript. All authors edited the manuscript. MB and ES designed the experiments. TT performed the experiments.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We thank the patient and family for participating in research. We thank the Jeffrey Modell Foundation for funding research on primary immunodeficiencies including the work presented here. The authors would like to thank the Genome Aggregation Database (gnomAD) and the groups that provided exome and genome variant data to this resource. A full list of contributing groups can be found at: https://gnomad.broadinstitute.org/about.

References

1. Puck JM. Population-based newborn screening for severe combined immunodeficiency: steps toward implementation. J Allergy Clin Immunol. (2007) 120:760–8. doi: 10.1016/j.jaci.2007.08.043

PubMed Abstract | CrossRef Full Text | Google Scholar

2. Dvorak CC, Haddad E, Buckley RH, Cowan MJ, Logan B, Griffith LM, et al. The genetic landscape of severe combined immunodeficiency in the United States and Canada in the current era (2010-2018). J Allergy Clin Immunol. (2019) 143:405–7. doi: 10.1016/j.jaci.2018.08.027

PubMed Abstract | CrossRef Full Text | Google Scholar

3. Haddad E, Logan BR, Griffith LM, Buckley RH, Parrott RE, Prockop SE, et al. SCID genotype and 6-month post-transplant CD4 count predict survival and immune recovery: a PIDTC retrospective study. Blood. (2018) 132:1737–49. doi: 10.1182/blood-2018-03-840702

CrossRef Full Text | Google Scholar

4. Fuchs S, Rensing-Ehl A, Erlacher M, Vraetz T, Hartjes L, Janda A, et al. Patients with T+/low NK+ IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency. Eur J Immunol. (2014) 44:3129–40. doi: 10.1002/eji.201444689

PubMed Abstract | CrossRef Full Text | Google Scholar

5. Recher M, Berglund LJ, Avery DT, Cowan MJ, Gennery AR, Smart J, et al. IL-21 is the primary common γ chain-binding cytokine required for human B-cell differentiation in vivo. Blood. (2011) 118:6824–35. doi: 10.1182/blood-2011-06-362533

PubMed Abstract | CrossRef Full Text | Google Scholar

6. Heimall J, Logan BR, Cowan MJ, Notarangelo LD, Griffith LM, Puck JM, et al. Immune reconstitution and survival of 100 SCID patients post hematopoietic cell transplant: a PIDTC natural history study. Blood. (2017) 130:2718–27. doi: 10.1182/blood-2017-05-781849

PubMed Abstract | CrossRef Full Text | Google Scholar

7. Pai SY, Logan BR, Griffith LM, Buckley RH, Parrott RE, Dvorak CC, et al. Transplantation outcomes for severe combined immunodeficiency, 2000-2009. N Engl J Med. (2014) 371:434–46. doi: 10.1056/NEJMoa1401177

PubMed Abstract | CrossRef Full Text | Google Scholar

8. Railey MD, Lokhnygina Y, Buckley RH. Long-term clinical outcome of patients with severe combined immunodeficiency who received related donor bone marrow transplants without pretransplant chemotherapy or post-transplant GVHD prophylaxis. J Pediatr. (2009) 155:834–40.e1. doi: 10.1016/j.jpeds.2009.07.049

CrossRef Full Text | Google Scholar

9. Stiehm ER, Lawlor GJ, Kaplan MS, Greenwald HL, Neerhout RC, Sengar DP, et al. Immunologic reconstitution in severe combined immunodeficiency wihout bone-marrow chromosomal chimerism. N Engl J Med. (1972) 286:797–803. doi: 10.1056/NEJM197204132861501

PubMed Abstract | CrossRef Full Text | Google Scholar

10. Stenson PD, Mort M, Ball EV, Shaw K, Phillips AD, Cooper DN. The human gene mutation database: building a comprehensive mutation repository for clinical and molecular genetics, diagnostic testing and personalized genomic medicine. Hum Genet. (2014) 133:1–9. doi: 10.1007/s00439-013-1358-4

PubMed Abstract | CrossRef Full Text | Google Scholar

11. Karczewski KJ, Francioli LC, Tiao G, Cummings BB, Alföldi J, Wang Q, et al. Variation across 141,456 human exomes and genomes reveals the spectrum of loss-of-function intolerance across human protein-coding genes. bioRxiv[preprint]. (2019) 531210. doi: 10.1101/531210

CrossRef Full Text | Google Scholar

12. Nourizadeh M, Borte S, Fazlollahi MR, Hammarström L, Pourpak Z. A new IL-2RG gene mutation in an X-linked SCID identified through TREC/KREC screening: a case report. Iran J Allergy, Asthma Immunol. (2015) 14:457–61. Available online at: http://ijaai.tums.ac.ir/index.php/ijaai/article/view/594

PubMed Abstract | Google Scholar

13. Buckley RH, Win CM, Moser BK, Parrott RE, Sajaroff E, Sarzotti-Kelsoe M. Post-transplantation B cell function in different molecular types of SCID. J Clin Immunol. (2013) 33:96–110. doi: 10.1007/s10875-012-9797-6

PubMed Abstract | CrossRef Full Text | Google Scholar

14. Mazzolari E, Forino C, Guerci S, Imberti L, Lanfranchi A, Porta F, et al. Long-term immune reconstitution and clinical outcome after stem cell transplantation for severe T-cell immunodeficiency. J Allergy Clin Immunol. (2007) 120:892–9. doi: 10.1016/j.jaci.2007.08.007

PubMed Abstract | CrossRef Full Text | Google Scholar

15. Miggelbrink AM, Logan BR, Buckley RH, Parrott RE, Dvorak CC, Kapoor N, et al. B cell differentiation and IL-21 response in IL2RG/JAK3 SCID patients after hematopoietic stem cell transplantation. Blood. (2018) 131:2967–77. doi: 10.1182/blood-2017-10-809822

PubMed Abstract | CrossRef Full Text | Google Scholar

16. Gatti R, Meuwissen H, Allen H, Hong R, Good R. Immunological reconstitution of sex-linked lymphopenic immunological deficiency. Lancet. (1968) 292:1366–9. doi: 10.1016/S0140-6736(68)92673-1

CrossRef Full Text | Google Scholar

17. Borghans JA, Bredius RG, Hazenberg MD, Roelofs H, Jol-Van Der Zijde EC, Heidt J, et al. Early determinants of long-term T-cell reconstitution after hematopoietic stem cell transplantation for severe combined immunodeficiency. Blood. (2006) 108:763–9. doi: 10.1182/blood-2006-01-009241

PubMed Abstract | CrossRef Full Text | Google Scholar

18. Benedicte N, Leroy S, Decaluwe H, Le Deist F, Picard C, Moshous D, et al. Long-term outcome after hematopoietic stem cell transplantation of a single-center cohort of 90 patients with severe combined immunodeficiency. Bone Marrow Transplant. (2009) 113:4114–24. doi: 10.1182/blood-2008-09-177923.The

CrossRef Full Text | Google Scholar

19. Patel NC, Chinen J, Rosenblatt HM, Hanson IC, Brown BS, Paul ME, et al. Long-term outcomes of nonconditioned patients with severe combined immunodeficiency transplanted with HLA-identical or haploidentical bone marrow depleted of T cells with anti-CD6 mAb. J Allergy Clin Immunol. (2008) 122:1185–93. doi: 10.1016/j.jaci.2008.10.030

PubMed Abstract | CrossRef Full Text | Google Scholar

20. Laffort C, Le Deist F, Favre M, Caillat-Zucman S, Radford-Weiss I, Debré M, et al. Severe cutaneous papillomavirus disease after haemopoietic stem-cell transplantation in patients with severe combined immune deficiency caused by common γc cytokine receptor subunit or JAK-3 deficiency. Lancet. (2004) 363:2051–4. doi: 10.1016/S0140-6736(04)16457-X

CrossRef Full Text | Google Scholar

21. Nowak K, Linzner D, Thrasher AJ, Lambert PF, Di WL, Burns SO. Absence of γ-chain in keratinocytes alters chemokine secretion, resulting in reduced immune cell recruitment. J Invest Dermatol. (2017) 137:2120–30. doi: 10.1016/j.jid.2017.05.024

PubMed Abstract | CrossRef Full Text | Google Scholar

22. Kamili Q, Seeborg FO, Saxena K, Nicholas SK, Banerjee PP, Angelo LS, et al. Severe cutaneous human papillomavirus infection associated with natural killer cell deficiency following stem cell transplantation for severe combined immunodeficiency. J Allergy Clin Immunol. (2014) 134:1451–4. doi: 10.1016/j.jaci.2014.07.009

PubMed Abstract | CrossRef Full Text | Google Scholar

23. Chahoud J, Semaan A, Chen Y, Cao M, Rieber AG, Rady P, et al. Association between β-genus human papillomavirus and cutaneous squamous cell carcinoma in immunocompetent individuals—a meta-analysis. JAMA Dermatol. (2016) 152:1354–64. doi: 10.1001/jamadermatol.2015.4530

PubMed Abstract | CrossRef Full Text | Google Scholar

24. Moon WY, Powis SJ. Does natural killer cell deficiency. (NKD) increase the risk of cancer? NKD may increase the risk of some virus induced cancer. Front Immunol. (2019) 10:1703. doi: 10.3389/fimmu.2019.01703

PubMed Abstract | CrossRef Full Text | Google Scholar

25. Myers LA, Patel DD, Puck JM, Buckley RH. Hematopoietic stem cell transplantation for severe combined immunodeficiency in the neonatal period leads to superior thymic output and improved survival. Blood. (2002) 99:872–8. doi: 10.1182/blood.V99.3.872

PubMed Abstract | CrossRef Full Text | Google Scholar

26. Ozaki K, Spolski R, Feng CG, Qi C-F, Cheng J, Sher A, et al. A critical role for IL-21 in regulating immunoglobulin production. Science. (2002) 298:1630–4. doi: 10.1126/science.1077002

PubMed Abstract | CrossRef Full Text | Google Scholar

27. Lim CK, Abolhassani H, Appelberg SK, Sundin M, Hammarström L. IL2RG hypomorphic mutation: Identification of a novel pathogenic mutation in exon 8 and a review of the literature. Allergy Asthma Clin Immunol. (2019) 15:1–8. doi: 10.1186/s13223-018-0317-y

PubMed Abstract | CrossRef Full Text | Google Scholar

28. Sharfe N, Shahar M, Roifman CM. An interleukin-2 receptor γ chain mutation with normal thymus morphology. J Clin Invest. (1997) 100:3036–43. doi: 10.1172/JCI119858

PubMed Abstract | CrossRef Full Text | Google Scholar

29. Pepper AE, Buckley RH, Small TN, Puck JM. Two mutational hotspots in the interleukin-2 receptor gene causing human X-linked severe combined immunodeficiency. Am J Hum Genet. (1995) 57:564–71.

PubMed Abstract | Google Scholar

Keywords: SCID, B cell, transplant, chimerism, IL2RG, IL-21, gamma chain, IVIG

Citation: Deal C, Thauland TJ, Stiehm ER, Garcia-Lloret MI and Butte MJ (2020) Intact B-Cell Signaling and Function With Host B-Cells 47 Years After Transplantation for X-SCID. Front. Immunol. 11:415. doi: 10.3389/fimmu.2020.00415

Received: 04 November 2019; Accepted: 21 February 2020;
Published: 20 March 2020.

Edited by:

Andrew R. Gennery, Newcastle University, United Kingdom

Reviewed by:

Mirjam van der Burg, Leiden University Medical Center, Netherlands
Stuart G. Tangye, Garvan Institute of Medical Research, Australia

Copyright © 2020 Deal, Thauland, Stiehm, Garcia-Lloret and Butte. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Manish J. Butte, mbutte@mednet.ucla.edu

These authors have contributed equally to this work