Sec. Antigen Presenting Cell Biology
Volume 11 - 2020 | https://doi.org/10.3389/fimmu.2020.586613
Roles of Ubiquitination and Deubiquitination in Regulating Dendritic Cell Maturation and Function
- 1Department of Laboratory Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, China
- 2International Genome Center, Jiangsu University, Zhenjiang, China
- 3Department of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
Dendritic cells (DCs) are specialized antigen-presenting cells that play a key role in immune homeostasis and the adaptive immune response. DC-induced immune tolerance or activation is strictly dependent on the distinct maturation stages and migration ability of DCs. Ubiquitination is a reversible protein post-translational modification process that has emerged as a crucial mechanism that regulates DC maturation and function. Recent studies have shown that ubiquitin enzymes, including E3 ubiquitin ligases and deubiquitinases (DUBs), are pivotal regulators of DC-mediated immune function and serve as potential targets for DC-based immunotherapy of immune-related disorders (e.g., autoimmune disease, infections, and tumors). In this review, we summarize the recent progress regarding the molecular mechanisms and function of ubiquitination in DC-mediated immune homeostasis and immune response.
Ubiquitination is a post-translational mechanism of protein modification that plays a crucial role in diverse biological processes by regulating the degradation and activity of substrate proteins (1, 2). The covalent conjugation of ubiquitin to lysine (K) residues of substrate proteins is mediated by an enzymatic reaction cascade. This process is catalyzed by the sequential activity of ubiquitin-activating (E1), ubiquitin-conjugating (E2), and ubiquitin-ligating (E3) enzymes (3). The substrate specificity of ubiquitination is primarily determined by E3 ubiquitin ligases, which recognize substrate proteins and catalyze the conjugation of ubiquitin to target proteins (3). The E3 ubiquitin ligases are a large, diverse group of proteins, characterized by one of several defining motifs. These have been historically grouped into two classes: the RING (really interesting new gene)–type E3 ubiquitin ligases and the HECT (homologous to the E6AP carboxyl terminus)–type E3 ubiquitin ligases (4). During the formation of polyubiquitin chains, the carboxyl-terminal glycine residue of ubiquitin is typically attached to an internal K residue or the amino-terminal methionine (M1) of another ubiquitin, which forms eight polyubiquitin chains, including K6, K11, K27, K29, K33, K48, K63, and M1 ubiquitin chains (5, 6). The type of polyubiquitin chains can influence the fate of the substrate, which exert distinct biological functions. For example, K48- and K11-linked polyubiquitin chains are usually involved in directing proteins for proteasome-dependent degradation, whereas K63-linked polyubiquitin chains have nonproteolytic functions, including the regulation of protein kinase activation and DNA damage repair (7). Ubiquitination is a reversible process that can be reversed by deubiquitination, which is performed by DUBs (8). DUBs can cleave conjugated ubiquitin chains from ubiquitinated substrates for signal transduction (8). Ubiquitination and deubiquitination play an essential role in the regulation of different aspects of immune function. The role of ubiquitination in lymphocyte biology has been reviewed elsewhere (6, 9–11). Therefore, this review will focus on dendritic cells (DCs).
DCs are specialized antigen-presenting cells that initiate and shape both the innate and adaptive immune responses (12). DCs arise from a hematopoietic lineage, which consist of developmentally and functionally distinct subsets. Under steady state conditions, DCs are customarily divided into conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Moreover, cDC subsets comprise type 1 cDCs (cDC1s/CD8α+/CD103+DCs) and type 2 cDCs (cDC2s/CD11b+DCs), which are specialized at priming CD8+ and CD4+ T cells, respectively (13). Under inflammatory conditions, circulating Ly6Chi monocytes directly differentiate into monocyte-derived DCs (MoDCs), which are induced by the inflammatory microenvironment and possess pro-inflammatory activity (13, 14). Recent studies have demonstrated that ubiquitination regulates DC-mediated immune homeostasis and adaptive immune responses. In this review, we describe the recent progress regarding the crucial role of ubiquitination in DC maturation and function (Table 1).
Table 1 Summary of the ubiquitin enzymes involved in regulating DCs maturation and function, as discussed in the text.
Molecular Mechanisms of Ubiquitination-Mediated DC Maturation
Regulation of MHCII and Costimulatory Molecules by Ubiquitination
Under physiological conditions, DCs maintain an immature or steady state to induce immune tolerance and maintain immune homeostasis (46). Upon toll-like receptor (TLR) stimulation, DCs undergo a transition from an immature to mature state and are accompanied by markedly upregulated membrane molecules, MHCII and costimulatory molecules, including CD80, CD86, and CD40 (13). Ubiquitination is an important mechanism that controls the surface expression and transport of MHCII by ubiquitin-dependent protein degradation in the lysosome (15, 16). The E3 ubiquitin ligase MARCH1, a member of the membrane-associated RING-CH (MARCH) family, ubiquitinates the β subunit of MHCII molecule via its cytoplasmic lysine (17). Apart from MHCII, MARCH1 also ubiquitinates CD86 in DCs by inducing intracellular degradation via the transmembrane domains (18, 19). Moreover, the transmembrane domains of MARCH1 interact but do not ubiquitinate CD83. CD83 competes with CD86 for interaction with MARCH1, thereby inhibiting CD86 ubiquitination and promoting the expression of CD86 on the surface of DCs (20). Overall, MARCH1 negatively regulates DC maturation by inducing the ubiquitin-dependent degradation of MHCII and CD86, and its expression is downregulated during DC maturation. In addition, ubiquitination can also affect DC maturation by regulating the level of MHCII transcription. Another E3 ubiquitin ligase, Hrd1, enhances MHCII gene transcription by promoting transcriptional repressor B lymphocyte-induced maturation protein (BLIMP) protein degradation through ubiquitination in DCs (29). A recent study shows that the NEDD4 family HECT E3 ubiquitin ligase WWP2 is required for SteD-dependent ubiquitination of mature MHCII during salmonella infection. As an adaptor, tumor suppressor protein TMEM127 binds to WWP2 and enables WWP2 to ubiquitinate MHCII, which contributes to the degradation of MHCII (28).
Regulation of NF-κB Activation by Ubiquitination
The canonical NF-κB signaling pathway is a key mediator of TLR-stimulated DC activation and functional maturation (47). Accumulating evidence suggests that ubiquitination plays a crucial role in the regulation of NF-κB signaling in both DC tolerance and activation. Several E3 ubiquitin ligases and DUBs in DCs regulate NF-κB activation by ubiquitination or deubiquitination of the NF-κB essential modulator NEMO (also known as IKK γ) and NF-κB subunits (p65 and c-Rel) (Figure 1). In general, TLR stimulation leads to the recruitment of the adaptor protein MyD88 and the kinases IRAK1 and IRAK4. Subsequent IRAKs now dissociate from the receptor complex and interact with E3 ubiquitin ligase TRAF6, which results in the K63 polyubiquitination of IRAK1/4 and TRAF6 itself by cooperating with the E2 ligases Ubc13 (48). As for TNFR stimulation, LUBAC (linear ubiquitin chain assembly complex) is required for full activation of NF-κB by the MyD88-dependent pathway. LUBAC complex extends K63-linked poly-ubiquitin chains with M1-linked poly-ubiquitin chains, resulting in recruitment and activation of the IKK complex through its adaptor NEMO, thereby liberating NF-κB transcription factors. In addition, three DUBs that play a crucial role in NF-κB signalling are OTULIN (OUT deubiquitinase with linear linkage specificity), CYLD (cylindromatosis), and A20 (48–50).
Figure 1 Ubiquitination regulating DCs activation and maturation by NF-κB signaling. Ubiquitination regulates both canonical and non-canonical NF-κB signaling in DCs. DCs are activated by toll-like ligands and inflammatory factors, such as GM-CSF, during an infection or inflammation. Activated DCs produce various cytokines, such as the proinflammatory cytokines IL-6, IL-12, and IL-23, which, in turn, regulate the differentiation of T cells. The deubiquitinase OTUB1 promotes canonical NF- κB activity by cleaving K48-linked polyubiquitination of UBC13. Rhbdd3 negatively regulates DC activation by recruiting A20 and facilitates A20-mediated deubiquitination of NEMO. CYLD inhibits activation of canonical NF-κB signaling by removing K63-linked polyubiquitin chains from NEMO. c-Cbl inhibits NF-κB signaling by stabilizing p105 and accumulating p50 via its RING domain, thus attenuating the recruitment of stimulatory NF-κB heterodimers and suppressing the activation of DCs. MKRN2 and PDLIM2 synergistically promote polyubiquitination and degradation of p65, thereby suppressing NF-κB-dependent activation of DCs. Trabid deubiquitinates and stabilizes Jmjd2d, a histone demethylase that removes transcriptionally repressive histone modifications, H3K9me2 and H3K9me3, from the Il12 and Il23 promoters to promote the recruitment of c-Rel, thereby facilitating the production of those cytokines in activated DCs. CRL4 DCAF2 negatively regulates IL-23 production in DCs by controlling NF-κB inducing kinase (NIK) stability and noncanonical NF-κB activation.
The ubiquitin-editing protein, A20, contains a deubiquitinase ovarian tumor (OTU) domain, which cleaves the K63-linked ubiquitin chains of NEMO, thereby inhibiting IKK and NF-κB activation (30, 51). It has been proposed that the rhomboid family protease, Rhbdd3, recruits A20 by binding K27-linked polyubiquitination on Lys302 of NEMO via the ubiquitin-binding-association (UBA) domain and facilitates A20-mediated deubiquitination of NEMO (31). An A20 or Rhbdd3 deficiency causes aberrant DC activation and increased production of the inflammatory cytokines, IL-12 and IL-6, in response to TLR ligands (31, 32). However, emerging evidence shows that the deubiquitinase activity of A20 is dispensable for NF-κB signaling (52). A20 negatively regulates TNF-induced NF-κB activation by binding and stabilizing linear ubiquitin chains (49, 50). Therefore, the precise molecular mechanisms that regulates TLR or TNF-induced NF-κB-dependent DC activation by A20 deserves further investigation. The nuclear protein, PDLIM2, as a nuclear E3 ubiquitin ligase, negatively regulates DC activation by promoting the p65 subunit of NF-κB polyubiquitination and proteasome-dependent degradation through its LIM (abnormal cell lineage 11-islet 1-mechanosensory abnormal 3) domain (34). The chaperone protein, HSP70, is required for the transport of the NF-κB-PDLIM2 complex to the proteasome (35). A deletion of PDLIM2 in DCs enhances the LPS-induced production of the proinﬂammatory cytokines, IL-6 and IL-12p40 (34). Interestingly, another study has reported that the E3 ubiquitin ligase, MKRN2, and PDLIM2 may synergistically promote polyubiquitination and p65 degradation, thereby suppressing NF-κB-dependent DC activation (34, 36). The E3 ubiquitin ligases, c-Cbl and Cbl-b, are two members of a highly evolutionary conserved family of Cbl proteins characterized as negative regulators of DC activation in vitro. c-Cbl inhibits the NF-κB pathway by stabilizing p105 and accumulating p50 via its RING domain, thereby attenuating the recruitment of stimulatory NF-κB heterodimers and suppressing the proinflammatory cytokine expression of IL-12 (40). Similarly, bone marrow–derived dendritic cells (BMDCs) from cbl-b deficient mice exhibit increased cytokine production (IL-1α, IL-6, and TNF-α) upon TLR stimulation. However, the molecular mechanisms by which Cbl-b is involved in DC activation remains largely unclear (41).
Jin et al. (37) reported that the deubiquitinase, Trabid, plays an essential role in mediating epigenetic regulation of TLR-induced expression of IL-12 and IL-23 in DCs. Another NF-κB family member, c-Rel, plays a key role in the TLR-induced expression of IL-12 and IL-23 (53, 54). Moreover, Trabid promotes the recruitment of c-Rel to the IL-12a, IL-12b, and IL-23a gene promoters by deubiquitinating and stabilizing the histone demethylase, Jmjd2d, which facilitates the production of these cytokines in activated DCs. Deubiquitination could also augment NF-κB- dependent DC activation by stabilizing the E2-conjugating enzyme. A recent study has demonstrated that OTUB1 promotes NF-κB activity in DCs by cleaving K48-linked polyubiquitination and increasing the stability of the E2-conjugating enzyme, UBC13 (38). UBC13 induces the activation of TGFβ-activated kinase 1 (TAK1) by ubiquitinating IL-1 receptor-associated kinase 1 (IRAK1) and TNF receptor-associated factor 6 (TRAF6), which is essential for NF-κB activity (55). In addition, a conditional deletion of OTUB1 in DCs impairs the TLR-induced activation of DCs and production of IL-12, IL-6, and TNF (38). Together, these data suggest a pivotal role for OTUB1 in driving NF-κB-dependent cytokine production by DCs. Another deubiquitinase, CYLD, is also an important regulator of NF-κB activity. Full-length CYLD inhibits activation of NF-κB signaling by removing K63-linked polyubiquitin chains from the signaling molecules, NEMO, TRAF2, and RIP1 (receptor-interacting protein 1) (56). However, the natural occurring short splice variant of CYLD (sCYLD), which lacks the TRAF2 and NEMO-binding sites, results in increased NF-κB activity (56). A conditional deletion of sCYLD in DCs augments the activation of CD8α+ DCs and the production of TNF, IL-10, and IL-12 in mice infected with Listeriosis. This suggests that this regulation depends on sCYLD‐mediated deubiquitination; however, the precise targets remain unclear (39).
Ubiquitination has also been found to play an important role in regulating the non-canonical NF-κB pathway involved in cytokine production by DCs. A recent study has revealed the E3 ubiquitin ligase, CRL4 DCAF2, is involved in GM-CSF-induced expression of the inflammatory cytokine, IL-23. CRL4 DCAF2 negatively regulates IL-23 production in DCs by controlling NF-κB–inducing kinase (NIK) stability and noncanonical NF-κB activation. Previous studies suggest that cellular inhibitor of apoptosis 1 and 2 (cIAP1/2) activation is mediated through its K63 ubiquitination by TRAF2 and promotes NIK degradation (57). Interestingly, CRL4 DCAF2 directly promotes polyubiquitination and subsequent degradation of NIK independent regulation of cIAP activity. The DC-conditional deletion of DCAF2 enhances IL-23 production in DCs and promotes the development of psoriasis (43). Collectively, CRL4 DCAF2 negatively regulates the production of the inflammatory cytokine, IL-23, in DCs by ubiquitinating NIK, implying a crucial role for CRL4 DCAF2 on the non-canonical NF-κB-dependent cytokine production in DCs (Figure 1).
Ubiquitination in DC-Mediated CD4 T Cell Responses
It has been widely accepted that DC are inducers of central and peripheral tolerance, which plays a crucial role in maintaining immune homeostasis and blocking autoimmune responses (58, 59). DC-induced immune tolerance primarily depends on signaling pathway-mediated maturation, cytokine production, and immunomodulatory molecule expression of DCs. Ubiquitination plays a key role in the regulation of DC-induced immune tolerance. The E3 ubiquitin ligase, CRL4 DCAF2, is critical for maintaining T cell homeostasis under physiological conditions. CRL4 DCAF2 ablation in DCs impairs T cell homeostasis and causes spontaneous autoimmunity in aging mice, which is characterized by the increased frequency of Th17 effector T cells in the inguinal lymph nodes and elevated anti-nuclear antibodies in the serum (43). In addition, the deubiquitinase, A20, has also been well-characterized for this function. As discussed above, A20 inhibits the MyD88-NF-κB signaling axis in DCs to prevent spontaneous DC activation and activated T cell expansion (33). While an A20 deficiency in DCs has little effect on T cell development, the mice with a conditional A20 deficiency in DCs spontaneously developed systemic autoimmunity, including lymphocyte-dependent colitis, sero-negative ankylosing arthritis, and stereotypical conditions for human inflammatory bowel disease (IBD) (33). In addition, DCs lacking A20 exhibit increased co-stimulatory molecules expression and induce the generation of in vivo self-reactive Th1 and Th17 cells, which was accompanied by a high level of IL-6 secretion (33). Another study showed that the expression of A20 in DCs both preserves T cell homeostasis, and also directly inhibits B cell activation in vitro (30). These findings indicate that DCs require A20 to preserve immune homeostasis and suggest that A20 may function as a crucial checkpoint in the development of systemic autoimmunity, which provides a potential target for the therapeutic intervention of autoimmune diseases.
Rhbdd3, an upstream regulator of A20, and A20 binding and inhibitor of NF-κB-1 (ABIN-1) are also important to DC-induced immune tolerance. Rhbdd3 recruits A20 to promote NEMO deubiquitination and thereby negatively regulates DC activation. Rhbdd3 knockout mice spontaneously develop a systemic autoimmune phenotype, which is characterized by increased serum concentrations of immunoglobulins and antibodies and a reduced frequency of splenic Tregs, implying that Rhbdd3 is involved in controlling immune homeostasis (31). Similarly, as a ubiquitin-binding protein, ABIN-1 expression in DCs is necessary to preserve immune homeostasis (60). The DC-specific deletion of ABIN-1 exhibits excessive activation of NF-κB and MAPK signaling and produces more inflammatory cytokines (TNF, IL-6, IL-12, and IL-23) in vitro. Consequently, DC-conditional ABIN-1 deficient mice develop splenomegaly and lymphadenopathy by three to four months of age, characterized by an accumulation of myeloid cells and activated T lymphocytes (60).
While a deficiency of Cbl‐b alone exhibits no obvious effects on DC development and function (41), it has been reported that mice which a double ablation of c-Cbl and Cbl-b in DCs results in the development of severe liver inflammation (42). Cbl mutant mice display significantly increased cDC1 in the peripheral lymphoid organs and liver, which exhibits a hyperactivated phenotype and mediates the systemic activation of T and B cells. Further studies have shown that Cbls promote FMS-like tyrosine kinase 3 (FLT3) signaling via the ubiquitination of FLT3. This research suggests that both c-Cbl and Cbl-b–mediated ubiquitination hold the key for DC subset homeostasis and immune quiescence under steady‐state conditions, implying that c-Cbl and Cbl-b may have the potential to be therapeutic targets for the treatment of DC-mediated liver inflammation (42).
The MHCII molecule is crucial for the antigen presentation function of DCs by displaying antigenic peptides to CD4 T cells, which facilitates their activation, proliferation, and differentiation (61). MARCH1-mediated ubiquitination degradation of this molecule is an important mechanism that controls the surface expression of MHCII in DCs (15, 16). In agreement with this finding, MARCH1 expression is inversed to the level of surface MHCII expression during DC maturation (21). Thus, MARCH1 may negatively regulate the antigen presentation capability of DCs by reducing the surface expression of MHCII under steady state conditions (17). However, DCs deﬁcient in MHCII ubiquitination do not present more antigen, but exhibit a low Ag-presenting ability in vivo (22). Moreover, MHCII knock-in mice whose MHCII was not ubiquitinated show DC dysfunction similar to that of MARCH1 knockout mice (22). Therefore, MARCH1 both negatively regulate the surface expression of MHCII, and maintain DC function in the steady state via MHC II ubiquitination (15, 16, 22). Tregs are potent immune regulatory cells that upregulate MARCH1 expression in DCs by IL-10. This upregulation increases MHCII ubiquitination and reduces the surface expression of MHCII, which consequently results in impaired antigen presentation by DCs (20, 23, 24). WWP2 has also been shown to ubiquitinate and deplete the surface expression of mature MHCII, thereby suppressing DC-mediated T cell activation during Salmonella infection (28).
CD4 T Cell Activation
In addition to affecting antigen presentation, MHCII ubiquitination can also regulate DC-mediated T-cell activation and development. However, the function of DC-mediated T-cell activation in MHCII ubiquitination appears to be highly complex. One study found that increased antigen presentation did not result in enhanced CD4 T cell activation (25). Moreover, MARCH1-deficient DCs have reduced, rather than increased, ability to activate naїve CD4+ T cells despite their higher level of surface MHCII expression (25). This finding may due to an excess accumulation of MHCII, which results in proteotoxicity and homeostatic disruption of the lipid raft and tetraspanin web in DCs (26). However, this has not been studied with mature naïve T cells, only for developing CD4+ thymocytes (26). In addition, MHCII ubiquitination is important for the development of thymic Tregs, but does not seem to affect the development of conventional T cells (62, 63). Ubiquitination also participates in DC-mediated T-cell activation by promoting MHCII gene transcription. The E3 ubiquitin ligase, Hrd1, increases MHCII expression through the ubiquitin-dependent degradation of BLIMP1. In addition, Hrd1 knockout mice exhibit impaired DC-mediated priming of CD4+ T cells and an attenuated autoimmune response (29).
As previously discussed, MARCH1 can regulate DC phenotypic maturation via ubiquitination-dependent degradation of the costimulatory molecule, CD86, thereby controlling DC-mediated T cell activation (18, 20). CD86 ubiquitination is an important mechanism of controlling the level of CD86 expression in DCs (19). DCs with ubiquitination-resistant mutant CD86 have greater T cell-activating abilities than that of DCs expressing wild-type CD86 in vitro (18). However, CD86 ubiquitination in DC-mediated T cell activation in vivo remains unknown.
CD4 T Cell Differentiation
In addition to serving as APCs, DCs are also key regulators of CD4+ T cell differentiation by secreting different cytokines (64). Ubiquitination regulates cytokine production by DCs in response to different types of pathogens, which, in turn, guides the differentiation of CD4+ T cells to the generation of effector T cells, including the Th1 and Th17 subsets of helper T cells. It has been established that IL-12 and IL-23 overproduction are involved in the pathogenesis of autoimmune diseases through controlling the differentiation of naїve CD4+ T cells toward the Th1 and Th17 lineages, respectively (65, 66). The deubiquitinase, Trabid, in DCs promotes the generation of Th1 and Th17 cells and EAE pathogenesis through the epigenetic regulation of IL-12 and IL-23 expression (37). The culture medium from LPS-stimulated Trabid-deficient BMDCs decreases the differentiation of CD4+ T cells into Th1 and Th17 cells in vitro due to reduced IL-12 and IL-23 production (37). Furthermore, mice with a conditional deletion of Trabid in DCs are resistant to the induction of experimental autoimmune encephalomyelitis (EAE), which display a significantly lower frequency of Th1 and Th17 cells in vivo. However, T cells with a conditional Trabid deficiency have no defects in the in vivo production of Th1 and Th17 cells or in vitro T cell responses. These data demonstrate that Trabid mediates the production of IL-12 and IL-23 in DCs, thereby promoting Th1 and Th17 differentiation (37). In contrast to the positive role of Trabid in Th17 differentiation, E3 ubiquitin ligase, CRL4 DCAF2, in DCs negatively regulates Th17 differentiation in vivo by limiting the production of IL-23 (43). Mechanistically, as discussed above, CRL4 DCAF2 negatively regulates noncanonical NF-κB activation by inducing NIK ubiquitination and degradation, thereby inhibiting IL-23 production in DCs. A conditional DC deficiency of CRL4 DCAF2 mice display substantially enhanced sensitivity to the induction EAE and have an increased frequency of Th17 inflammatory effector cells in the peripheral lymphoid organs (43).
A20 is another Th17-regulatory factor identified to be a deubiquitinase of NEMO, which regulates TLR- and TNF-triggered NF-κB activation (67). The DC-specific deletion of A20 promotes Th17 cell production both in vivo and in vitro through secreting high amounts of IL-23 (30). Intestinal DCs from DC-conditional A20 knockout mice have enhanced and biased abilities to promote inflammatory Th1 and Th17 cell differentiation, which is associated with lymphocyte-dependent colitis (68). In addition, Rhbdd3 recruits A20 to inhibit IL-6 production in DCs, and thus suppresses the generation of Th17 cells. Rhbdd3-deficient mice display increased susceptibility to Th17 cell-mediated colitis (31).
Ubiquitination in DC-Mediated Cross-Priming of the CD8 T Cell Response
Antigen cross-presentation is crucial for an effective CD8+ T cell response in both intracellular bacterial infections and cancer (69). DCs have superior antigen cross-presentation ability to cross-prime CD8+ T cells both in vivo and in vitro (70, 71). Moreover, a recent study has revealed ubiquitination to be an important mechanism of regulating DC cross-presentation (44). The deubiquitinating enzyme, UCH-L1, has been shown to promote DC cross-presentation both in vitro and in vivo. In addition, UCH-L1 knockout mice infected with listeria monocytogenes exhibit impaired DC-mediated cross-priming of the CD8+ T cell response. Mechanistically, UCH-L1 promotes DC cross-presentation not by favoring antigen uptake or phagosome maturation, but by facilitating the recycling of MHC class I (MHC I) molecules. Reduced UCH-L1 expression in DCs impairs the colocalization of intracellular MHC I with late endosomal/lysosomal compartments, which is required for antigen cross-presentation. This data reveals that UCH-L1 expression in DCs plays a critical role in modulating DC-mediated cross-priming of the CD8+ T cell response, which facilitates the exploration of potential targets for therapeutic intervention against various infections and cancers. However, the substrate and E3 ubiquitin ligase for UCH-L1 in DCs remains to be defined.
Ubiquitination in DC Migration
The migratory ability of DCs is key for the initiation of protective pro-inflammatory and tolerogenic immune responses (72). Apart from glycolytic metabolism and epigenetic pathways (73, 74), ubiquitination is also involved in the regulation of the migration of different DC subsets in health and disease (27, 45). A CRL5 complex, Cullin RING Ligase CRL5 ASB2α, which is highly expressed in immature DCs and down-regulated following DC maturation, which promotes DC migration by facilitating cell spreading and the formation of adhesive structures in immature DCs. Further research reveals that CRL5 ASB2α triggers polyubiquitylation and drives proteasome-mediated degradation of actin-binding protein filamins (FLNs), which are major organizers of the actin cytoskeleton (45). Interestingly, MARCH1-mediated MHCII ubiquitination is essential for the antigen presentation function of DCs but is also required for the migration of CD206-expressing monocyte-derived DCs (CD206+ MoDCs) to skin-draining lymph nodes (sdLNs) (27). The expression of IFN regulatory factor (IRF) 4 and C-C Motif Chemokine Receptor 7 (CCR7) play a pivotal role in controlling DC migration (72). CD206+ MoDCs from MARCH1 knockout mice exhibit MHCII overexpression and decreased IRF4 and CCR7 expression and have reduced migratory ability from the skin to sdLNs. Moreover, GM-CSF could restore CD206+ MoDC migration by promoting IRF4 expression both in vitro and in vivo. Collectively, these data suggest that the downregulation of MHCII by ubiquitination is crucial for the migration of CD206+ MoDCs to sdLNs in health and disease. While enhanced DC migration during early stages of the immune response is essential for the rapid induction of the immune response to eliminate invading pathogens, the timely termination of DC trafﬁcking at the late stage of the inﬂammatory response is required to prevent unwanted inﬂammation (74, 75). While current observations have primarily focused on the positive regulation of ubiquitination in DC migration, whether ubiquitination or deubiquitination can also function as a negative mediator that suppresses DC migration requires further investigation.
Conclusion and Future Perspectives
In this review, ubiquitination was identified to play a critical role in DC maturation and function. To date, multiple E3 ubiquitin ligases and DUBs have been identified as key regulators of different physiological aspects of DCs, ranging from DC maturation to DC-mediated immune homeostasis and adaptive immune responses. These ubiquitin enzymes also play a key role in pathological processes mediated by DCs, indicating that they can be potential therapeutic targets for DC-based treatment of autoimmune diseases and cancer. The development of E3 ubiquitin ligase and DUB inhibitors with high selectivity should be considered as a promising immunotherapy approach. Bortezomib (or PS-341, trade name Velcade) is the first drug approved for targeting the UPS for the treatment of multiple myeloma (76). Several derivatives of bortezomib are at various stages of clinical trials for treatment of tumors (77). However, therapeutic approaches that selectively target the DCs ubiquitin-proteasome system in cancer and other diseases need to be further developed.
It has been well-established that ubiquitin enzymes are key players in the ubiquitination process; however, a major challenge in future studies will be to elucidate their underlying molecular mechanisms. For example, how each type of ubiquitin chain is generated, hydrolyzed, and recognized in DCs remains poorly understood. Whether acetylation and phosphorylation are involved in the translational modifications of ubiquitin remains unknown. Identification of the substrates of these enzymes is another challenge. Moreover, different DC subsets have both shared and unique functions; however, it remains largely unknown whether/how ubiquitin enzymes regulate the function of different DC subsets.
BZ and LZ drafted the manuscript. LX and YX discussed and revised the manuscript. QY and KR designed the study and revised the manuscript. All authors contributed to the article and approved the submitted version.
This work was supported by the National Natural Science Foundation of China (Grant No. 81701612) and the Natural Science Foundation of Jiangsu (Grant No. BK20170563).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: ubiquitination, dendritic cells, DC maturation, immune responses, post-translational modification
Citation: Zhu B, Zhu L, Xia L, Xiong Y, Yin Q and Rui K (2020) Roles of Ubiquitination and Deubiquitination in Regulating Dendritic Cell Maturation and Function. Front. Immunol. 11:586613. doi: 10.3389/fimmu.2020.586613
Received: 23 July 2020; Accepted: 19 October 2020;
Published: 16 November 2020.
Edited by:Elodie Segura, Institut Curie, France
Reviewed by:Pierre G. Lutz, Centre National de la Recherche Scientifique (CNRS), France
Haiyin Liu, The University of Melbourne, Australia
Copyright © 2020 Zhu, Zhu, Xia, Xiong, Yin and Rui. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ke Rui, email@example.com; Qing Yin, firstname.lastname@example.org