Abstract
Complex interactions between bacteriophages and their bacterial hosts play significant roles in shaping the structure of environmental microbial communities, not only by genetic transduction but also by modification of bacterial gene expression patterns. Survival of phages solely depends on their ability to infect their bacterial hosts, most importantly during phage entry. Successful dynamic adaptation of bacteriophages when facing selective pressures, such as host adaptation and resistance, dictates their abundance and diversification. Co-evolution of the phage tail fibers and bacterial receptors determine bacterial host ranges, mechanisms of phage entry, and other infection parameters. This review summarizes the current knowledge about the physical interactions between tailed bacteriophages and bacterial pathogens (e.g., Salmonella enterica and Pseudomonas aeruginosa) and the influences of the phage on host gene expression. Understanding these interactions can offer insights into phage–host dynamics and suggest novel strategies for the design of bacterial pathogen biological controls.
INTRODUCTION
Bacteriophages (or phages) are the most abundant and most diversified microorganisms on Earth. Acting as obligate bacterial predators, phage can be found in all reservoirs populated by bacterial hosts, e.g., in soil (Marsh and Wellington, 1994; Kimura et al., 2008), in aquatic environments (Bergh et al., 1989; Suttle, 2007), and even in the human gut (Breitbart et al., 2003; Mills et al., 2013). The evolutionary success of bacteriophages, estimated at approximately an order of magnitude and up to a 25-fold greater abundance in comparison with their bacterial hosts (Fuhrman, 1999), indicates remarkable dynamic adaptability of phages in nature.
In natural habitats, phages and bacteria are in a constant arms race that proceeds in continuous cycles of co-evolution (Thingstad and Lignell, 1997). As bacteria develop mechanisms to prevent phage infection, e.g., bacterial receptor modification and degradation of invading phage DNA (Labrie et al., 2010), phages can circumvent the resistance and evolve mechanisms to target such resistant bacteria (Samson et al., 2013). This arms race continues and become one of the major forces to both expand global genetic diversity and maintain balance within microbial communities.
Previous reviews of phage–bacteria interaction mainly focused on model organisms such as Escherichia coli phages (or coliphages) and bacterial CRISPR/Cas immunity systems against phage genome replication. The focal points of this review, however, are physical interactions between tailed phages and other Gram-negative bacterial pathogens, specifically Salmonella enterica and Pseudomonas aeruginosa, and the influences of phage–host interactions on the gene expression of these clinically important bacteria and, more generally, on microbial diversity.
PHYSICAL INTERACTIONS BETWEEN TAILED PHAGES AND THEIR HOSTS
To initiate infection, phages need to adsorb to the host surfaces, penetrate cell walls and inject genetic materials into the host. Mechanisms used to initiate the connection to bacterial hosts prior to phage genome injection are referred to as tails. Ackermann and Prangishvili (2012) demonstrates that over ninety percent of approximately 6,200 phages examined by electron microscopy (EM) are tailed phages in the Caudovirales order (i.e., siphophages, myophages, and podophages). As the name implies, the adsorption machinery dedicated for specific host recognition is localized at the tail-end, varying from a simple tail tip to a complex base plate. Such mechanisms appear to be well-correlated with host adsorption strategies (Veesler and Cambillau, 2011; Fokine and Rossmann, 2014). Some tail structures can be quite complex and include extra-structures including tail spikes and tail fibers (Fokine and Rossmann, 2014). This adsorption machinery is the most rapidly evolving part of the tailed phage genome (Hendrix et al., 1999). Tail proteins of these phages are diverse and capable of recognizing almost every host surface component (Figure 1), including surface proteins, polysaccharides and lipopolysaccharides (LPS; Lindberg, 1973).
FIGURE 1
The interactions between phages and hosts occur between phage tail proteins and bacterial receptors, which are proteins or LPS. These interactions determine host specificity and range. Known receptors (Table 1) of Salmonella phages are flagella proteins (e.g., FliC and FljB, Shin et al., 2012; and FliK, Choi et al., 2013), outer membrane porin or osmoregulatory protein OmpC (Ho and Slauch, 2001; Marti et al., 2013), outer membrane protein for vitamin B12 uptake BtuB (Kim and Ryu, 2011), outer membrane protein for drug efflux pump TolC (Ricci and Piddock, 2010), outer membrane transport protein FhuA (Casjens et al., 2005), O-antigen of LPS (Shin et al., 2012), and Vi capsular antigen (Pickard et al., 2010). On the other hand, known phage receptors in P. aeruginosa are type IV pili (Heo et al., 2007; Bae and Cho, 2013) and O-antigen of LPS (Le et al., 2013).
Table 1
| Specific host receptors | Reference | |
|---|---|---|
| S. enterica | Flagellar proteins | |
| FliC and FljB | Shin et al. (2012) | |
| FliK | Choi et al. (2013) | |
| Outermembrane proteins | ||
| OmpC | Ho and Slauch (2001), Marti et al. (2013) | |
| BtuB | Kim and Ryu (2011) | |
| TolC | Ricci and Piddock (2010) | |
| FhuA | Casjens et al. (2005) | |
| Surface antigens | ||
| O-antigen | Shin et al. (2012) | |
| Vi-antigen | Pickard et al. (2010) | |
| P. aeruginosa | Surface antigens | |
| O-antigen | Le et al. (2013) | |
| Vi-antigen | Temple et al. (1986), Hanlon et al. (2001) | |
| Type IV pili | ||
| PilA | Bae and Cho (2013), Heo et al. (2007) |
Specific host receptors for Salmonella and P. aeruginosa phages.
The protein–protein interactions between phage tail fiber proteins and bacterial flagella filaments are specific and strong. Such flagellatropic phages adsorb to the bacterial hosts via flagellin proteins, i.e., phase 1 antigen FliC, phase 2 antigen FljB, or flagella hook FliK (Shin et al., 2012; Choi et al., 2013). The preference for each flagella receptor varies among phages. Salmonella siphophages can use only FliC or both FliC and FljB as receptors (Shin et al., 2012). The latter is more prevalent as variety in host receptors leads to wider possible host ranges. In addition to being flagellotropic, host adsorption in some phages is also dependent upon motility and the directional rotation of the flagella themselves. For instance, Salmonella phage iEPS5 requires adsorption to either FliC or FljB flagellin and successful host infection only occurs if the flagella are rotating counter-clockwise in the presence of flagella torque protein MotA and hook protein FliK (Choi et al., 2013). The evidence also suggests that, alongside the adsorption step, injection of iEPS5 DNA is also flagella-dependent, possibly through the flagellin channel (Choi et al., 2013). In addition to adsorption through flagella, phages can also attach through the pili. Specifically, P. aeruginosa phage MPK7 and M22 utilize type IV pili as their receptors. P. aeruginosa hosts lacking pilA gene are resistant to infection by these phages (Heo et al., 2007; Bae and Cho, 2013).
Besides motility apparatus such as flagella and pili, outer membrane proteins are also targets for phage adsorption. OmpC porin is used as a receptor by Salmonella Gifsy and T4-like phages (Ho and Slauch, 2001; Marti et al., 2013), while vitamin B12 uptake protein BtuB is used by T5-like phages (Kim and Ryu, 2011). Although resistance to BtuB-targeting phages can develop in Salmonella, the trait is not heritable and bacterial daughter cells can revert and become susceptible to these phages again (Kim and Ryu, 2011). Additionally, a conserved innate efflux pump TolC is used by Salmonella phage as a receptor (Ricci and Piddock, 2010). TolC has also been shown to be a colonization factor of S. Typhimurium, in which a tolC null strain is virulence attenuated (Webber et al., 2009). Therefore, application of TolC targeting phages is expected to have dual advantages, as the phages themselves can directly infect the bacterial host and can also drive the emergence of TolC-deficient Salmonella, which is incapable of colonization and incapable of spreading infection. Like coliphages, Salmonella phages use ferrichrome transport protein FhuA as a receptor (Casjens et al., 2005).
Bacteriophages also use surface antigens such as O-and Vi-antigens as receptors. In both S. enterica and P. aeruginosa, O-antigens of LPS are phage receptors. In Salmonella, tail spike proteins of the typical siphophages or podophages not only recognize but also hydrolyze the O-antigen, allowing the phage to penetrate through the 100-nm O-antigen layer during infection (Andres et al., 2012). Siphophage SSU5, however, uses core oligosaccharides of LPS as receptors (Kim et al., 2014), making SSU5 a beneficial addition to the phage cocktail against insensitive Salmonella populations capable of O-antigen glucosylation (Kim and Ryu, 2012). Other than O-antigens, Vi capsular antigens of S. Typhi are also targets for recognition by phage tail protein, specifically at a conserved acyl esterase domain (Pickard et al., 2010). On the other hand, phages of P. aeruginosa recognize O-antigens of LPS using tail fibers (Le et al., 2013). Interestingly, extracellular matrix-like structures such as alginate glycopolysaccharide layers are also found in Pseudomonas spp. The role of these layers is to physically alter phage accessibility. Phage F116 of Pseudomonas can produce alginate lyase which dissolves the alginate layer and facilitates penetration and dispersion of phages in such a matrix (Hanlon et al., 2001). However, alteration in alginate production renders Pseudomonas insensitive to phage2 and φPLS-I, thus revealing the role of alginate in adhesion (Temple et al., 1986).
It is important to note that interactions between phages and host bacteria are not exclusive to single types of phage protein-receptor recognition. Shin et al. (2012) demonstrated cross-infection and cross-resistance among phages recognizing different targets on S. enterica, e.g., bacterial hosts that are resistant to flagellatropic phages are sensitive to phages targeting BtuB and LPS. Conversely, due to possible interactions between BtuB and LPS targets, bacterial strains that are resistant to BtuB-targeting and LPS-targeting phages are susceptible to flagellatropic phages (Shin et al., 2012). According to the “killing the winner” hypothesis (Thingstad and Lignell, 1997), cross-infection by different types of phages naturally limits the abundance of successful strains and thereby increases bacterial diversity. Although a vast variations of phage tails and other adsorption structures have been observed by EM (Ackermann and Prangishvili, 2012), the X-ray structures of phage tail proteins including Dit and gp27-like proteins from various phages demonstrate remarkable structural similarities which suggest a common evolutionary origin for phage tail proteins (Veesler and Cambillau, 2011).
PHAGE–BACTERIA: GENETIC GIVE-AND-TAKE
Rapid reciprocal evolutionary competition between bacteria and phages (and even among phages with common bacterial hosts in a shared environment) creates high selective pressure, forcing diversification of the attachment-related structures and the emergence of various phage infection tactics (as reviewed in Veesler and Cambillau, 2011). However, success in infection is apparently not sufficient for phage survival in nature. As an obligate parasite, phages are dependent upon the survival of their host population. Thus, the availability of hosts is at least as important in determining the environmental fitness of phages. Phages must also modulate the gene expression of host cells to prevent superinfection by other phages.
In a lysogenic life cycle, phage integrates its genome and replicates along with its host. Not only the host survival is sustained, the presence of prophages has also been repeatedly shown to be beneficial. Prophages can provide their host population with immunity against secondary infection or superinfection by other incoming phages. In lysogenic S. enterica, expression of podophage P22-encoded proteins SieA and SieB exclude superinfection through degradation of the superinfecting phage genome (Susskind et al., 1974a) and induce lysis of superinfected host cells, protecting the whole host population (Susskind et al., 1974b). Similarly, the expression of siphophage HK97-encoded protein gp15 protein excludes superinfection by other HK97 phages via inhibition of phage genome entry (Cumby et al., 2012).
Beyond superinfection exclusion, prophages can also enhance the fitness of their hosts in the environment. A recent study in P. aeruginosa PAO1 has shown that infection with LPS-targeting E79 myophage for 4 days gave rise to E79-immune variants that, while exhibiting slower growth than control PAO1 and impairment of twitching, swimming and swarming abilities, produced more virulence factor pyocyanin and were less frequently internalized by J774 macrophages (Hosseinidoust et al., 2013). In addition, several lines of evidence demonstrate extensive genetic circulation amongst phage and host communities. In fact, approximately 20% of the extant genetic content of a given bacterial species are acquired (Gogarten et al., 2002). These acquired genetic materials include so-called mobile genetic elements, including several bacterial pathogenicity islands which can potentially be horizontally transferred across species boundaries. Some phage-related chromosomal islands use phages as transduction vectors (Novick et al., 2010). This phage-mediated horizontal transfer of genetic material is of extreme importance as it acts as an active driving force behind bacterial evolution. Classic examples are the P. aeruginosa gigantic bacteriocin called R- and F-type pyocins which are utilized to combat other microbes in the community (Nakayama et al., 2000). High sequence similarities and striking structural parallels between the phage tail structures and bacterial pyocins reveal a clear evolutionary connection between these complex molecular devices (Nakayama et al., 2000). Another phage-like structure found in bacteria including P. aeruginosa is a dynamic bacterial type VI secretion system (T6SS) used for translocation of virulence factors into target cells: the same mechanism the phage uses to transfer its genome to the host (Basler et al., 2012). Moreover, a recent report has shown that phage tail-like structures produced by marine bacterium Pseudoalteromonas luteoviolacea can trigger metamorphosis of a marine tubeworm, providing novel insights into the intricate interaction between phage, bacterium and animal (Shikuma et al., 2014).
Taken together, it is clear that phages play key roles in shaping the evolution of bacteria and vice versa. Phages not only manipulate expression of bacterial genes but also provide competitiveness for their hosts to thrive in the ecosystem as that would mutually be beneficial for both parties. Substantial structural and mechanistic analogies among bacterial puncturing devices, pyocins and secretion systems indicate an intimate evolutionary relationship between phages and their hosts.
PHAGES IN MICROBIAL ECOSYSTEMS
Extensive in vitro characterization of host–phage relationships indicates short-term arms races are run between hosts and parasites (Hall et al., 2011); however, in natural habitats the interaction continues over extended time-periods. A recent study proposed that the co-evolution of phages and soil microbial communities is more likely driven by fluctuating selection dynamics, which can continue indefinitely (Gómez and Buckling, 2011). In a large ecosystem, an immune bacterial population gains benefit from the bactericidal effects of phages that act against other competitive species in the same pool and selectively enrich the ecosystem. It may therefore be better to view phage–host relationships as not merely parasitic but as mutualistic (Williams, 2013).
In an attempt to better understand phage–bacteria population dynamics, extensive metagenomic studies have been performed to characterize microbial communities in various niches, including marine, soil, and animal hosts. Metagenomic studies of human feces have demonstrated high abundance and diversity of phages (Minot et al., 2011, 2013). Barr et al. (2013) demonstrated that the abundance of phages on mucosal surfaces of epithelial cells can provide defense against bacterial infection, suggesting a plausible model for phage therapy against host mucosal infection such as foodborne infection by S. enterica or lung infection by P. aeruginosa.
As naturally designed predators to balance bacterial ecology, and at a time when multi-drug resistant bacteria are becoming widespread, phages have been identified as potential antimicrobial agents for biocontrol applications in various applications: medical therapy, agribusiness, and food safety. In the case of S. enterica, there have been reports of the use of phages as antimicrobial testing agents in areas ranging from food-producing animals to ready-to-eat foods (Wall et al., 2010; Guenther et al., 2012). In the case of P. aeruginosa phages, many reports have suggested that phages can be used to treat P. aeruginosa infected wounds and to disrupt the bacterial biofilm (recently reviewed in Wittebole et al., 2014). Nonetheless, it is crucial to remain aware of host–phage dynamics when developing phage therapies. The typical structure of phage–host populations in fluctuating selection dynamics is that immune bacteria are present in high abundance and are infected by wide host range phages while sensitive bacteria are much less abundant and are infected by both wide and narrow host range phages (Flores et al., 2011). Narrow host range phages are needed and must be carefully selected to ensure that the low abundance bacterial population has no change at resurgence.
NEW RESEARCH APPROACHES IN PHAGE–HOST INTERACTION
In terms of host–phage interaction, structural biology and microbiology are counterparts. Although several detailed structures of bacteriophages have been determined at near atomic resolution by cryoEM and image analysis (e.g., Figures 2A,B; Lander et al., 2012; Guo et al., 2013; Zhang et al., 2013), concern has been raised regarding poor data quality in EM of bacteriophages (Ackermann, 2014). It is also important to keep in mind that identified host–phage interactions are based on a limited number of microbial systems. It is indeed a challenge to catch the action of bacteriophages in their native niches. The invention of a small portable cryo-plunger for cryoEM that allows vitrification of environmental sample on site has opened new opportunities to merge the strengths of metagenomics and of cryoEM (Comolli et al., 2012; Luef et al., 2013). Combining metagenomics and cryogenic electron tomography (cryoET), an imaging technique suitable for visualizing the 3D organization of a pleiomorphic structure at moderate resolution, will reveal unexplored worlds within the microbial community. In fact, cryoET has been used to capture the extensive structural rearrangement of virus particles and their molecular machineries, e.g., during T7 phage infection (Figure 2C; Hu et al., 2013), the assembly of cyanophage (Dai et al., 2013) and the formation of virus-associated pyramids (VAPs) for the egression of archaeal virus (Daum et al., 2014).
FIGURE 2
In addition, the cryoEM 3D reconstruction of phages and virus can be vastly improved by averaging taking advantage of the icosahedral symmetry of the virus (Guo et al., 2013; Li et al., 2013; Zhang et al., 2013). Unfortunately, applying symmetry will completely mask all the unique non-symmetrical features such as base plates and tails. Of interest, a new type of extremely sensitive complementary metal oxide semiconductor (CMOS) detector has recently been developed (McMullan et al., 2009). This detector allows correction for drift and beam-induced motions of the specimen, thus images can be acquired at higher resolution. Bai et al. (2013) were able to reconstruct the 3D asymmetrical structure of ribosome at near atomic resolution using only 30,000 particles. Currently, more routine image processing and data acquisition protocols are being refined for advanced single particle analysis (Bai et al., 2013; Li et al., 2013). Combining single particle EM with biochemical experiments, 3D snapshots of bacteriophages trapped in various states can potentially be achievable at near atomic resolution.
Moreover, an electron-optical equivalent of “phase contrast” light microscopy called “phase plate” is being developed. Various prototypical versions of phase plate have been shown to improve contrast in EM significantly (Danev and Nagayama, 2011; Glaeser, 2013). Glaeser et al. (2013) showed that, with phase plate, small proteins such as individual streptavidin tetramers can be clearly distinguished in cryo. Furthermore, using phase contrast cryoEM, the 3D structure of epsilon15 bacteriophage can be resolved at nanometer resolution even with no symmetry imposed (Murata et al., 2010). Remarkably, structural details such as the tail and capsid of an individual phage can be directly delineated by electron cryo-tomographic reconstruction (Murata et al., 2010). Once these elaborate EM instruments are in place, they will enable not only a relatively routine 3D rendition of phages at high resolution using single particle analysis, but will also elucidate phage structural dynamics in the physiological environment.
Advances in metagenomics analyses and sequencing technologies have not only led to a much better appreciation of the abundance of uncultivable environmental microbial populations, but they have also enabled the monitoring of community dynamics (Breitbart and Rohwer, 2005; Edwards and Rohwer, 2005; Suttle, 2005; Rosario and Breitbart, 2011; Breitbart, 2012; Duhaime and Sullivan, 2012). However, only a handful of insights on the novel phage species can be gained through such techniques. Combining metagenomic analysis with correlative light-EM, which allows visualization of the same sample with both fluorescent and EM (Knierim et al., 2012; Luef et al., 2013; Chang et al., 2014; Schorb and Briggs, 2014), will lead to a “visual metagenomics” that will drive the genomic identification and structural characterization of novel uncultivable phages. Clearly, this is just the beginning of a new era of phage research.
CONCLUSIONS AND PERSPECTIVES
In this review, we summarize the physical interactions between tailed phages and commonly found Gram-negative bacterial pathogens, specifically S. enterica and P. aeruginosa, which can be used as models, for other pathogens in the development of phage therapeutic strategy. The ongoing evolutionary battles between phages and bacteria continue to shape microbial communities. As the co-evolution continues, bacteria develop resistance and exploit phage machinery to fight other bacteria in order to increase environmental fitness while phages manipulate host behaviors by alteration of bacterial gene expression. Recent advances in metagenomic analysis open new doors to the vast resources of genetic diversity of viromes from various habitats, while cryoET gives much clearer pictures of how phages and hosts interact at the molecular level. Functional characterization of phages in the laboratory, combined with cryoET and subtomogram averaging, provide snapshots of different stages in phage infection. Furthermore, recent developments in direct-electron detection technology, phase plate and single particle EM provide routine protocols for determination of 3D structures of phages at near-atomic resolution. These could potentially revolutionize our understanding of the complex interplay between phages and hosts in their natural ecosystems, which is of fundamental importance in phage use in biocontrol and therapeutic strategies.
Statements
Acknowledgments
Soraya Chaturongakul acknowledges the support by the Center of Excellence on Environmental Health, Toxicology and Management of Chemicals (ETM). Puey Ounjai acknowledges support from the Talent Management Program of Mahidol University.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Summary
Keywords
bacteriophage, host–phage interaction, phage resistance mechanism, host–phage dynamics, microbial community
Citation
Chaturongakul S and Ounjai P (2014) Phage–host interplay: examples from tailed phages and Gram-negative bacterial pathogens. Front. Microbiol. 5:442. doi: 10.3389/fmicb.2014.00442
Received
30 April 2014
Accepted
04 August 2014
Published
20 August 2014
Volume
5 - 2014
Edited by
Luis Raul Comolli, Lawrence Berkeley National Laboratory, USA
Reviewed by
Steven Ripp, University of Tennessee, USA; Joanne B. Emerson, University of California, Berkeley, USA
Copyright
© 2014 Chaturongakul and Ounjai.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Puey Ounjai, Department of Biology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand e-mail: puey.oun@mahidol.ac.th
This article was submitted to Terrestrial Microbiology, a section of the journal Frontiers in Microbiology.
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