Impact Factor 4.076

The 3rd most cited journal in Microbiology

This article is part of the Research Topic

Biological hazards in food

Mini Review ARTICLE

Front. Microbiol., 28 August 2015 | https://doi.org/10.3389/fmicb.2015.00873

Presence of toxic microbial metabolites in table olives

  • Food Biotechnology Department, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, Seville, Spain

Table olives have an enormous importance in the diet and culture of many Mediterranean countries. Albeit there are different ways to produce this fermented vegetable, brining/salting, fermentation, and acidification are common practices for all of them. Preservation methods such as pasteurization or sterilization are frequently used to guarantee the stability and safety of fermented olives. However, final products are not always subjected to a heat treatment. Thus, microbiota is not always removed and appropriate levels of acidity and salt must be obtained before commercialization. Despite the physicochemical conditions not being favorable for the growth of foodborne pathogens, some illness outbreaks have been reported in the literature. Street markets, inappropriate manipulation and storage conditions were the origin of many of the samples in which foodborne pathogens or their metabolites were detected. Many authors have also studied the survival of pathogens in different styles of table olive elaboration, finding in general that olive environment is not appropriate for their presence. Inhibitory compounds such as polyphenols, low availability of nutrients, high salt content, low pH levels, bacteriocins, or the addition of preservatives act as hurdles against undesirable microorganisms, which contribute to obtaining a safe and good quality product.

Production of Table Olives

The fermentation of olive fruit has many centuries of history, particularly in the Mediterranean basin, where this fermented vegetable has had a great influence on the culture and diet of many countries. According to the last consolidated statistics of the International Olive Council, worldwide production currently exceeds 2.4 million tons per year. Spain, Turkey, Egypt, Syria, Algeria, Greece, and Morocco are among the main producers, albeit Argentina, Peru, and USA are also important contributors (International Olive Council [IOC], 2015). Thus, table olive processing is spread worldwide and represents an important economic source for olive-growing countries.

Olive fruit cannot be consumed directly from the tree due to its peculiar characteristics (presence of the bitter glucoside compound oleuropein, high fat, and low sugar content). For this reason, diverse methods were developed to make them palatable. Although many of them share the general process of brining/salting, fermentation, and acidification, they can differ slightly between areas of production. The Trade Standard Applying to Table Olives (International Olive Council [IOC], 2004) defines table olives as: ‘the product obtained from suitable olive cultivars, processed to remove their natural bitterness, and preserved (by natural fermentation, heat treatment or preservatives) with or without brine until consumption.’ Among the most important table olive industrial processing methods we can find: (i) the so-called Spanish-style (alkali treated green olives), which represent about 50–60% of production, (ii) the so-called Californian-style (ripening of olives by alkaline oxidation), and (iii) directly brined olives (green, changing color or naturally black fruits) (Garrido-Fernández et al., 1997).

In all table olive processing methods described above, microorganisms have an important role, determining the safety, quality and flavor of the final product. Lactic acid bacteria (LAB) and yeasts are considered beneficial microorganisms, opposite to the role played by Enterobacteriaceae and Propionibacteriaceae (Garrido-Fernández et al., 1997; Arroyo-López et al., 2012; Hurtado et al., 2012). Traditionally, olive fermentation occurred spontaneously, but the process is not fully predictable and sometimes can lead to product spoilage or sanitary risks (Lanza, 2013). The present mini-review deals with the biological hazards posed by microorganisms in table olives, as well as the diverse hurdles that olive fermentation environments offer against growth of undesirable microorganisms.

Sanitary Risks Caused by Microorganisms or Their Metabolites in Table Olives

Despite fermented table olives having a long history of microbial safety, diverse biological hazards may be present in the finished product (Table 1). Among the most relevant, we can mention:

TABLE 1
www.frontiersin.org

TABLE 1. Summary of the main types of biohazards reported in table olives.

(i) Biogenic Amines

The consumption of foods containing high amounts of toxic biogenic amines may cause food intoxication and intolerance, with diverse associated symptoms such as migraines, headaches, depression, diarrhea, insomnia, etc., indicating the need for a better hygiene process. These compounds can be formed in table olives by spoilage microorganisms with amino acid decarboxylase activity. Hornero-Mendez and Garrido-Fernández (1994) reported the presence of biogenic amines (putrescine, cadaverine, and tyramine) in fermented green table olives with “zapatera” spoilage. The concentration of biogenic amines can increase during olive storage but the levels found in the final products are usually low and should not represent a health concern (García-García et al., 2001). Recently, Tofalo et al. (2012) also detected in naturally fermented olives a low quantity of biogenic amines, as well as the presence by RT-qPCR of biogenic amines producing bacteria.

(ii) Mycotoxins

These compounds are secondary toxic metabolites produced by some species of mold (mainly Aspergillus, Penicillium, and Fusarium genera) under aerobic and humidity conditions (El Adlouni et al., 2006). Mycotoxins in foods can be of concern for consumers, causing disease in human and other vertebrates with symptoms such as skin irritation, immunosuppression, neurotoxicity, etc. Contamination of table olives with various types of mycotoxins (Ochratoxin, Aflatoxin B, and Citrinin) have been documented in cracked olives (Franzetti et al., 2011), but Greek-style black olives are the most affected (Gourama and Bullerman, 1988; Ghitakou et al., 2006). Fortunately, mycotoxin levels usually found in table olives are too low to cause disease.

(iii) Foodborne Pathogenic Bacteria

Diverse works have reported the presence of Listeria monocytogenes (Caggia et al., 2004; RASFF Portal, 2012a), Staphylococcus aureus (Asehraou et al., 1992; Pereira et al., 2008), and Enterobacteriaceae species such as Yersinia enterocolitica and Escherichia coli (Asehraou et al., 1992; Franzetti et al., 2011; Lucena-Padrós et al., 2014) in table olives. However, there are no reports of illness outbreaks caused by these microorganisms in table olives. Botulism, associated with Clostridium botulinum growth, is certainly the most relevant biohazard in table olives. Diverse outbreaks associated with homemade table olives and recalls of suspected products have been reported (Debord et al., 1920; Fenicia et al., 1992; Cawthorne et al., 2005; Jalava et al., 2011; Pingeon et al., 2011; RASFF Portal, 2012b). It should be emphasized that artisanal productions or inadequate storage (pH ≥ 4.5 units) were often the origin of these outbreaks. Table 2 shows the epidemiological cases of botulism reported in table olives.

TABLE 2
www.frontiersin.org

TABLE 2. Major illness outbreaks associated with botulism in table olives.

(iv) Degradation of Organic Acids

Spoilage microorganisms associated with fermented vegetables such as Lactobacillus buchneri are able to produce acetic acid from lactic acid consumption under anaerobic conditions (Johanningsmeier and McFeeters, 2013), whilst Propionibacterium and Pectinatus species are able to convert lactic acid to propionic acid (Breidt et al., 2013; Lucena-Padrós et al., 2014). Oxidative yeasts can also consume the lactic and acetic acids produced during olive fermentation under aerobic conditions (Ruiz Cruz and González Cancho, 1969). However, they are not able to use these acids in the absence of oxygen. Lactic acid consumption in table olives reduces the preservative power of fermented olives and increases the pH values, which can allow for the growth of others undesirable microorganisms with the consequent loss of product quality and food safety.

Table Olives: Hurdles against Biological Hazards

The olive fermentation process led by LAB involves the consumption of sugars to produce a wide range of final products with preservative effects; among the most important is lactic acid. These preservative compounds, together with low pH, protein and vitamin content, as well as reduced water activity (chloride salt is added to brine in a range of 5–11%), provide an acidic and salty environment which is adverse for the growth of undesirable microorganisms.

Other compounds excreted by microorganisms can also act as biopreservative agents. Bacteriocins are bacterial proteins or peptides that show a bactericide effect against closely related species. Jimenez-Díaz et al. (1993) isolated a bacteriocin producer Lactobacillus plantarum strain from green olive fermentation. The inhibitory compounds produced by this microorganism (plantaricins S and T) were active against bacteria that can cause spoilage in olive fermentations (Propionibacteriaceae and Clostridium) as well as natural competitors of Lactobacillus plantarum in olive fermentation brines (Ruíz-Barba et al., 1994). Likewise, yeasts produce toxic proteins or glycoproteins, also known as killer factors, are able to inhibit the growth of fungi and other non-desirable yeast species acting as biocontrol agents. Debaryomyces, Pichia, and Candida are genera with a considerable number of killer strains isolated from table olives (Hernández et al., 2008).

Table olive fermentations also contain antimicrobial compounds that limit the growth of LAB and others microorganisms, mainly in non-alkali treated olives (Medina et al., 2010). It has been recently demonstrated that some phenolic and oleosidic substances such as the dialdehydic form of decarboxymethyl elenolic acid (EDA), as well as EDA linked to hydroxytyrosol (Hy-EDA) present in olive brines, possess significant bacteriocide activity against foodborne pathogens, even greater than other phenolic compounds isolated from foods or synthetic biocides (Medina et al., 2009; Brenes et al., 2011). Thus, survival studies carried out with E. coli O157:H7 in Spanish-style table olive fermentation, show inhibition of the pathogen in all assayed conditions (Spyropoulou et al., 2001). Similar behavior was observed in the survival of Bacillus cereus in green olive fermentation, where the population declined steadily during the fermentation process (Panagou et al., 2008). Recently, Grounta et al. (2013) investigated the survival of diverse foodborne pathogens artificially inoculated on natural black table olives. They demonstrated that natural black olives are not a favorable environment to support the growth of the assayed pathogens, and the population of all them showed a rapid decline throughout the first 2 days of storage. Medina et al. (2013) studied the survival of diverse food-borne pathogens (E. coli, Salmonella enterica, Listeria monocytogenes, and S. aureus) in industrial olive brines from different cultivars (Manzanilla, Gordal, Hojiblanca, etc.). They found a correlation with the presence of polyphenols, considered inhibitory compounds from olives fruit. 5-log reduction of population inoculated was achieved between 5 min to 17 days in the least deleterious brine. Hence, according to the available data, table olive industrial brines of different olive cultivars and elaboration processes, do not constitute a favorable environment for any of the pathogenic bacteria tested.

How to Reduce Biological Hazards in Table Olives

The objective of table olive producers should be to achieve zero risk in the case of illness and injury caused by toxic microbial metabolites. This can only be achieved by following practices that ensure that the fruits selected for processing are: produced under Good Agricultural Practices (GAP); processed under the principles of Good Manufacturing Practices (GMP) and produced at premises with equipment and personnel strictly following Good Hygienic Practices (GHP). All these requisites must be considered in the framework of food safety management systems, which include not only the HACCP System, but also other food defense tools to prevent intentional adulterations, i.e., CARVER (Criticality Accesibility Recuperability Vulnerability Effect Recognizability), TACCP (Threat Assessment and Critical Control Points), VACCP (Vulnerability Analysis and Critical Control Points).

In many cases, the fermentation of olive fruit still occurs spontaneously, which can sometimes lead to spoilage of the final product or to sanitary risks. In order to prevent these problems, the processing can be controlled through physicochemical (addition of acids, salt, temperature control, preservatives, or application of modified atmospheres) or microbiological approaches. To improve fermentation and consistently produce high quality, safe, final products; many authors have recommended strict process control of the above parameters, in addition to the use of starter cultures (see Corsetti et al., 2012 for a complete review on this aspect). The search for starters with application in olive fermentation and vegetables in general, has for many years, been focused on the activity of LAB and their technological applications. However, in the last decade, several publications have emphasized the importance of the role that selected yeasts can play when used as starter cultures during table olive processing (Arroyo-López et al., 2012; Bevilacqua et al., 2012, 2013). Moreover, the selection of microorganisms as starters in olive fermentation and vegetables in general, has been exclusively based on diverse technological criteria (homo-fermentative metabolism; high acidification rate and fast consumption of fermentable substrates; organic acids, polyphenols, high pH and salt tolerance; flavor development or production of bacteriocins) (Duran-Quintana et al., 1999; Sánchez et al., 2001; Corsetti et al., 2012; Hurtado et al., 2012; Di Cagno et al., 2013; Heperkan, 2013). However, in addition to technological characteristics, recent studies on the development of starter cultures for table olives have focused on the study of the probiotic potential of native microorganisms. These studies must include both LAB and yeasts for the development of a mixed-multifunctional starter, in order to improve and expand the form of action of the culture by the use of two complementary microorganisms with different properties.

Conclusion

The harsh environmental conditions found in the fermentation process (low pH, high salt content, presence of inhibitory compounds, sugar consumption, etc.), and the presence of other additional hurdles (production of bacteriocins, killer factors, addition of preservatives, etc.), make table olives an adverse habitat for the development of foodborne pathogens. If such growth ultimately occurs, the presence of undesirable microorganisms or their metabolites is often linked to the storage or selling conditions, not to the fermentation/production process. For all the above mentioned reasons, this widespread Mediterranean fermented vegetable can be considered quite a safe product, if good hygiene and manufacturing practices are followed and appropriated levels of salt (>5%) and pH (<4.3) are obtained in the final products.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This paper has received funding from Junta de Andalucía regional Government through the PrediAlo project (AGR-7755: www.predialo.science.com.es) and EU’s Seventh Framework Program (FP7/2007-2013) under grant agreement n°243471 (PROBIOLIVES: www.probiolives.eu). FA-L wishes to express thanks to the Spanish government for his Ramón y Cajal postdoctoral research contract, while EM-P would like to thank The Junta de Andalucía for his postdoctoral research contract. Both authors also express their gratitude to Antonio de Castro and Antonio Garrido-Fernández from Instituto de la Grasa (CSIC, Seville, Spain) for their invaluable comments and help during the writing of this manuscript.

References

Arroyo-López, F. N., Romero-Gil, V., Bautista-Gallego, J., Rodríguez-Gómez, F., Jiménez-Díaz, R., García García, P., et al. (2012). Potential benefits of the application of yeast starters in table olive processing. Front. Microbiol. 3:161. doi: 10.3389/fmicb.2012.00161

PubMed Abstract | CrossRef Full Text | Google Scholar

Asehraou, A., Faid, M., and Jana, M. (1992). Physico-chemical properties and the microflora of Moroccan black table olives. Grasas Aceites 43, 130–133. doi: 10.3989/gya.1992.v43.i3.1164

CrossRef Full Text | Google Scholar

Bevilacqua, A., Beneduce, L., Sinigaglia, M., and Corbo, M. R. (2013). Selection of yeasts as starters cultures for table olives. J. Food Sci. 78, M742–M751. doi: 10.1111/1750-3841.12117

PubMed Abstract | CrossRef Full Text | Google Scholar

Bevilacqua, A., Corbo, M. R., and Sinigaglia, M. (2012). Selection of yeasts as starters cultures for table olives: a step-by-step procedure. Front. Microbiol. 3:194. doi: 10.3389/fmicb.2012.00194

PubMed Abstract | CrossRef Full Text | Google Scholar

Breidt, F., Medina, E., Wafa, D., Pérez-Díaz, I., Franco, W., Huang, H., et al. (2013). Characterization of cucumber fermentation spoilage bacteria by enrichment culture and 16S rDNA cloning. J. Food Sci. 78, 470–476. doi: 10.1111/1750-3841.12057

PubMed Abstract | CrossRef Full Text | Google Scholar

Brenes, M., García, A., De los Santos, B., Medina, E., Romero, C., Castro, A., et al. (2011). Olive glutaraldehyde-like compounds against plant pathogenic bacteria and fungi. Food Chem. 125, 1262–1266. doi: 10.1016/j.foodchem.2010.10.055

PubMed Abstract | CrossRef Full Text | Google Scholar

Caggia, C., Randazzo, C. L., Salvo, M., Romeo, F., and Giudici, P. (2004). Occurrence of Listeria monocytogenes in green table olives. J. Food Prot. 10, 2189–2194.

PubMed Abstract | Google Scholar

Cawthorne, A., Celentano, L. P., D’Ancona, F., Bella, A., Masari, M., Aniballi, F., et al. (2005). Botulism and preserved green olives. Emerg. Infect. Dis. 11, 781–782. doi: 10.3201/eid1105.041088

PubMed Abstract | CrossRef Full Text | Google Scholar

Corsetti, A., Perpetuini, G., Schirone, M., Tofalo, R., and Suzzi, G. (2012). Application of starter cultures to table olive fermentation: an overview on the experimental studies. Front. Microbiol. 3:248. doi: 10.3389/fmicb.2012.00248

PubMed Abstract | CrossRef Full Text | Google Scholar

Debord, G. G., Edmondson, R. B., and Thom, C. (1920). Summary of bureau of chemistry investigations of poisoning due to ripe olives. J. Ame. Med. Assoc. 74, 1220–1221. doi: 10.1001/jama.1920.02620180018004

CrossRef Full Text | Google Scholar

Di Cagno, R., Coda, R., De Angelis, M., and Gobbetti, M. (2013). Exploitation of vegetables and fruits through lactic acid fermentation. Food Microbiol. 33, 1–10. doi: 10.1016/j.fm.2012.09.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Duran-Quintana, M. C., García-García, P., and Garrido-Fernández, A. (1999). Establishment of conditions for green table olive fermentation at low temperature. Int. J. Food Microbiol. 51, 133–143. doi: 10.1016/S0168-1605(99)00123-3

PubMed Abstract | CrossRef Full Text | Google Scholar

El Adlouni, C., Tozlovanu, M., Naman, F., Faid, M., and Pfohl-Leszkowicz, A. (2006). Preliminary data on the presence of mycotoxins (ochratoxin A, citrinin and aflatoxin B1) in black table olives “Greek style” of Moroccan origin. Mol. Nutr. Food Res. 50, 507–512. doi: 10.1002/mnfr.200600055

PubMed Abstract | CrossRef Full Text | Google Scholar

Fenicia, L., Ferrini, A. M., Aureli, P., and Padovan, M. T. (1992). Epidemia di botulismo da olive nere. Industrie Alimentari 31, 307–308.

Google Scholar

Franzetti, L., Scarpellini, M., Vecchio, A., and Planeta, D. (2011). Microbiological and safety evaluation of green table olives marketed in Italy. Ann. Microbiol. 61, 843–851. doi: 10.1007/s13213-011-0205-x

CrossRef Full Text | Google Scholar

García-García, P., Brenes-Balbuena, M., Romero-Barranco, C., and Garrido-Fernández, A. (2001). Biogenic amines in packed table olives and pickles. J. Food Prot. 64, 374–378.

PubMed Abstract | Google Scholar

Garrido-Fernández, A., Fernández-Díez, M. J., and Adams, R. M. (1997). Table Olives Production and Processing. London: Chapman & Hall. doi: 10.1007/978-1-4899-4683-6

CrossRef Full Text | Google Scholar

Ghitakou, S., Koutras, K., Kanellou, E., and Markaki, P. (2006). Study of aflatoxin B1 and ochratoxin A production by natural microflora and Aspergillus parasiticus in black and green olives of Greek origin. Food Microbiol. 23, 612–621. doi: 10.1016/j.fm.2005.12.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Gourama, H., and Bullerman, L. B. (1988). Mycotoxin production by molds isolated from “Greek-style” black olives. Int. J. Food Microbiol. 6, 81–90. doi: 10.1016/0168-1605(88)90087-6

CrossRef Full Text | Google Scholar

Grounta, A., Nychas, G. J. E., and Panagou, E. Z. (2013). Survival of food-borne pathogens on natural black table olives after post-processing contamination. Int. J. Food Microbiol. 161, 197–202. doi: 10.1016/j.ijfoodmicro.2012.12.017

PubMed Abstract | CrossRef Full Text | Google Scholar

Heperkan, D. (2013). Microbiota of table olive fermentation and criteria of selection for their use as starters. Front. Microbiol. 4:143. doi: 10.3389/fmicb.2013.00143

PubMed Abstract | CrossRef Full Text | Google Scholar

Hernández, A., Martín, A., Córdoba, M. G., Benito, M. J., Aranda, E., and Pérez-Nevado, F. (2008). Determination of killer activity in yeasts isolated from the elaboration of seasoned green table olives. Int. J. Food Microbiol. 121, 178–188. doi: 10.1016/j.ijfoodmicro.2007.11.044

PubMed Abstract | CrossRef Full Text | Google Scholar

Hornero-Mendez, D., and Garrido-Fernández, A. (1994). Biogenic amines in table olives. Analysis by high-performance liquid chromatography. Analyst 119, 2037–2041. doi: 10.1039/an9941902037

CrossRef Full Text | Google Scholar

Hurtado, A., Requant, C., Bordons, A., and Rozès, N. (2012). Lactic acid bacteria from fermented olives. Food Microbiol. 31, 1–8. doi: 10.1016/j.fm.2012.01.006

PubMed Abstract | CrossRef Full Text | Google Scholar

International Olive Council [IOC]. (2004). Trade standard applying to table olives. Madrid: International Olive Council.

International Olive Council [IOC]. (2015). World Table Olive Figures. Available at: http://www.internationaloliveoil.org/estaticos/view/132-world-table-olive-figures [accessed 15 July, 2015].

Jalava, K., Selby, K., Pihlajasaari, A., Kolho, E., Dahlsten, E., Forss, N., et al. (2011). Two Cases of Food-Borne Botulism in Finland Caused by Conserved Olives. Available at: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20034

Google Scholar

Jimenez-Díaz, R., Ríos-Sánchez, R. M., Desmazeaud, M., Ruíz-Barba, J. L., and Piard, J. C. (1993). Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated from a green olive fermentation. Appl. Envirom. Microbiol. 59, 1416–1424.

PubMed Abstract | Google Scholar

Johanningsmeier, S. D., and McFeeters, R. F. (2013). Metabolism of lactic acid in fermented cucumbers by Lactobacillus buchneri and related species, potential spoilage organisms in reduced salt fermentations. Food Microbiol. 35, 129–135. doi: 10.1016/j.fm.2013.03.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Lanza, B. (2013). Abnormal fermentation in table-olive processing: microbial origin and sensory evaluation. Front. Microbiol. 4:91. doi: 10.3389/fmicb.2013.00091

PubMed Abstract | CrossRef Full Text | Google Scholar

Lucena-Padrós, H., González, J. M., Caballero-Guerrero, B., Ruiz-Barba, J. L., and Maldonado-Barragán, A. (2014). Propionibacterium olivae sp. nov. and Propionibacterium damnosum sp. nov., isolated from spoiled packaged Spanish-style green olives. Int. J. Syst. Evol. Microbiol. 64, 2980–2985. doi: 10.1099/ijs.0.063032-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Medina, E., Brenes, M., García, A., Romero, C., and de Castro, A. (2009). Bactericidal activity of glutaraldehyde-like compounds from olives products. J. Food Prot. 72, 2611–2614.

PubMed Abstract | Google Scholar

Medina, E., Brenes, M., Romero, C., Ramirez, E., and de Castro, A. (2013). Survival of foodborne pathogenic bacteria in table olive brines. Food Control 34, 719–724. doi: 10.1016/j.foodcont.2013.06.026

CrossRef Full Text | Google Scholar

Medina, E., Gori, C., Servili, M., de Castro, A., Romero, C., and Brenes, M. (2010). Main variables affecting the lactic acid fermentation of table olives. Int. J. Food Sci. Technol. 45, 1291–1296. doi: 10.1111/j.1365-2621.2010.02274.x

CrossRef Full Text | Google Scholar

Panagou, E. Z., Tassou, C. C., Vamvakoula, P., Saravanos, E. K. A., and Nychas, G.-J. E. (2008). Survival of Bacillus cereus vegetative cells during Spanish-style fermentation of Conservolea green olives. J. Food Prot. 71, 1393–1400.

PubMed Abstract | Google Scholar

Pereira, A. P., Pereira, J. A., Bento, A., and Estevinho, M. L. (2008). Microbiological characterization of table olives commercialized in Portugal in respect to safety aspects. Food Chem. Toxicol. 4, 2895–2902. doi: 10.1016/j.fct.2008.05.033

PubMed Abstract | CrossRef Full Text | Google Scholar

Pingeon, J. M., Vanbockstael, C., Popoff, M. R., King, L. A., Deschamps, B., Pradel, G., et al. (2011). Two Outbreaks of Botulism Associated with Consumption of Green Olive Paste. Available at: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20035

Google Scholar

RASFF Portal. (2012a). Reference Number 2012.0703. Available at: https://webgate.ec.europa.eu/rasff-window/portal

RASFF Portal. (2012b). Reference Number 2012.1059. Available at: https://webgate.ec.europa.eu/rasff-window/portal

Ruíz-Barba, J. L., Cathcart, D. P., Warner, P. J., and Jimenez-Díaz, R. (1994). Use of Lactobacillus plantarum LPCO10, a bacteriocin producer, as a starter culture in Spanish-style green olive fermentations. Appl. Envirom. Microbiol. 60, 2059–2064.

PubMed Abstract | Google Scholar

Ruiz Cruz, J., and González Cancho, F. (1969). Methabolism of yeasts isolated from brines of Spanish-style table olives. I. the assimilation of lactic, acetic and citric acid. Grasas Aceites 20, 6–11.

Google Scholar

Sánchez, A. H., Rejano, L., Montaño, A., and De Castro, A. (2001). Utilization at high pH of starter cultures of lactobacilli for Spahish-style green olive fermentation. Int. J. Food Microbiol. 67, 115–122. doi: 10.1016/S0168-1605(01)00434-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Spyropoulou, K. E., Chorianopoulos, N. G., Skandamis, P. N., and Nychas, G.-J. E. (2001). Survival of Escherichia coli O157:H7 during the fermentation of Spanish-style green table olives (conservolea variety) supplemented with different carbon sources. Int. J. Food Microbiol. 66, 3–11. doi: 10.1016/S0168-1605(00)00510-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Tofalo, R., Schirone, M., Perpetuini, G., Angelozzi, G., Suzzi, G., and Corsetti, A. (2012). Microbiological and chemical profiles of naturally fermented table olives and brines from different Italian cultivars. Int. J. General Mol. Microbiol. 102, 121–131. doi: 10.1007/s10482-012-9719-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: microbial risk, foodborne pathogens, table olives, mycotoxins, Clostridium, biogenic amines

Citation: Medina-Pradas E and Arroyo-López FN (2015) Presence of toxic microbial metabolites in table olives. Front. Microbiol. 6:873. doi: 10.3389/fmicb.2015.00873

Received: 03 June 2015; Accepted: 10 August 2015;
Published: 28 August 2015.

Edited by:

Maria Schirone, University of Teramo, Italy

Reviewed by:

George-John Nychas, Agricultural University of Athens, Greece
Rosalba Lanciotti, University of Bologna, Italy

Copyright © 2015 Medina-Pradas and Arroyo-López. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Francisco Noé Arroyo-López, Food Biotechnology Department, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas – Pablo de Olavide University, Building 46, Ctra. Utrera, Km 1, 41013 Seville, Spain, fnarroyo@cica.es