Corrigendum: Morphologically Distinct Escherichia coli Bacteriophages Differ in Their Efficacy and Ability to Stimulate Cytokine Release In Vitro
- Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden
by Khan Mirzaei, M., Haileselassie, Y., Navis, M., Cooper, C., Sverremark-Ekström. E., and Nilsson, A. S. (2016). Front. Microbiol. 7:437. doi: 10.3389/fmicb.2016.00437
In the original article, it was suggested that bacterial debris controls exhibited no cytokine response when incubated with PBMC. However, a subsequent data audit and additional statistical analysis has revealed that a number of the bacterial debris controls exhibited a positive cytokine response whereas others not, resulting in an inflated mean particularly for the TNF response (Supplementary Table 1). These means were not significantly different to the response generated by the purified phages. This does not impact on the data presented or the statistical analysis that has been performed as part of Figure 2 (analysis was compared to commercial LPS or medium only) or any of the other analysis performed as part of the manuscript. However, it does mean that the bacterial debris controls are not suitable for showing the efficacy of the phage purification process and as such a component of the cytokine response generated may be due to remaining bacterial debris as suggested by Dufour et al. (2016).
Therefore, the sentence “In addition, no response to the purified bacterial debris when incubated with PBMC was observed (data not shown)” (page 4, end of second paragraph) is inaccurate and should consequently not be considered when evaluating the effect of phage preparations on human cell lines as described in our article.
In addition, there is an error in the figure legend of Figure 3A. The colors used to differentiate between the HT-29 and Caco-2 cells have become inverted.
The authors apologize for these two mistakes. While the first does not impact the conclusions from the statistical analyses that have been performed, as the comparisons of immune response of phage preparations by PBMCs were made against a standardized LPS control and not against the purified bacterial debris controls, it does support the suggestion made by Dufour et al. (2016) that at least some component of the observed cytokine response generated by the phage preparations may be due to residual contaminants.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/article/10.3389/fmicb.2016.02145/full#supplementary-material
Dufour, N., Henry, M., Ricard, J. D., and Debarbieux, L. (2016). Commentary: morphologically distinct Escherichia coli bacteriophages differ in their efficacy and ability to stimulate cytokine release in vitro. Front. Microbiol. 7:1029. doi: 10.3389/fmicb.2016.01029
Keywords: pharmacokinetics, phage therapy, cytokines, immune response, multi-resistant bacteria
Citation: Khan Mirzaei M, Haileselassie Y, Navis M, Cooper C, Sverremark-Ekström E and Nilsson AS (2017) Corrigendum: Morphologically Distinct Escherichia coli Bacteriophages Differ in Their Efficacy and Ability to Stimulate Cytokine Release In Vitro. Front. Microbiol. 7:2145. doi: 10.3389/fmicb.2016.02145
Received: 24 November 2016; Accepted: 20 December 2016;
Published: 04 January 2017.
Edited and reviewed by: Pilar García, Spanish National Research Council, Spain
Copyright © 2017 Khan Mirzaei, Haileselassie, Navis, Cooper, Sverremark-Ekström and Nilsson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Anders S. Nilsson, firstname.lastname@example.org
†Present Address: Mohammadali Khan Mirzaei, Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada