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About The Foodborne Pathogen Campylobacter

Original Research ARTICLE

Front. Microbiol., 18 May 2017 | https://doi.org/10.3389/fmicb.2017.00913

Profiling of Campylobacter jejuni Proteome in Exponential and Stationary Phase of Growth

Hana Turonova1, Nabila Haddad2, Mathieu Hernould2, Didier Chevret3, Jarmila Pazlarova1 and Odile Tresse2*
  • 1Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Czechia
  • 2SECALIM UMR1014, Institut National de la Recherche Agronomique, Nantes, France
  • 3UMR1319 MICALIS, Plateforme d'Analyse Protéomique de Paris Sud-Ouest, Institut National de la Recherche Agronomique, Jouy-en-Josas, France

Campylobacter jejuni has been reported as a major cause of bacterial food-borne enteritides in developed countries during the last decade. Despite its fastidious growth requirements, including low level of oxygen and high level of CO2, this pathogen is able to persist in the environment without permanent loss of its viability and virulence. As C. jejuni is not able to multiply outside a host, the cells spend significant amount of time in stationary phase of growth. The entry into the stationary phase is often correlated to resistance to various stresses in bacteria. The switching between exponential and stationary phases is frequently mediated by the regulator sigma S (RpoS). However, this factor is absent in C. jejuni and molecular mechanisms responsible for transition of cells to the stationary phase remain elusive. In this work, proteomic profiles of cells from exponential and stationary phases were compared using 2-D electrophoresis (2DE) fingerprinting combined with mass spectrometry analysis and qRT-PCR. The identified proteins, whose expression differed between the two phases, are mostly involved in protein biosynthesis, carbon metabolism, stress response and motility. Altered expression was observed also in the pleiotropic regulator CosR that was over-expressed during stationary phase. A shift between transcript and protein level evolution of CosR throughout the growth of C. jejuni was observed using qRT-PCR and (2DE). From these data, we hypothesized that CosR could undergo a negative autoregulation in stationary phase. A consensus sequence resulting from promoter sequence alignment of genes potentially regulated by CosR, including its own upstream region, among C. jejuni strains is proposed. To verify experimentally the potential autoregulation of CosR at the DNA level, electrophoretic mobility shift assay was performed with DNA fragments of CosR promoter region and rCosR. Different migration pattern of the promoter fragments indicates the binding capacity of CosR, suggesting its auto-regulation potential.

Introduction

Campylobacter jejuni is continuously reported as the main cause of bacterial food-borne infections in developed countries (EFSA and ECDC, 2015). The disease caused by this pathogen, namely campylobacteriosis, is triggered mainly by consumption of contaminated food or water, although a direct transmission from infected animals to human hosts can occasionally occur (Bronowski et al., 2014). It manifests as an acute inflammatory diarrhea with symptoms common to other bacterial enteritides—abdominal pain, fever and watery diarrhea often accompanied with the presence of blood and leukocytes in stool (Blaser and Engberg, 2008). In most cases, campylobacteriosis is self-limiting and does not require specific therapy, however severe autoimmune disorders, such as Guillain-Barré and Miller-Fisher syndromes (Salloway et al., 1996; Nachamkin, 2002), reactive arthritis (Pope et al., 2007), and inflammatory bowel disease (Rodriguez et al., 2006) may appear. These late-onset complications, together with long convalescence time and high occurrence of campylobacteriosis, are the reasons why the disease is ranked as an infection with one of the highest annual burden (Batz et al., 2012; Gibney et al., 2014; Mangen et al., 2015). Although various strategies have been adopted by member states of EU in order to decrease numbers of campylobacteriosis (Lin, 2009; Saxena et al., 2013), the prevalence of this disease remains very high. It is therefore necessary to identify genetic and environmental factors affecting the persistence of C. jejuni in the environment, in order to develop new methods mitigating the campylobacteriosis cases.

As a pathogen with fastidious growth requirements, C. jejuni puzzles scientists with its ability to withstand broad range of stresses encountered during its lifecycle. In other pathogens represented by Escherichia coli, Salmonella spp., Shigella spp., and Vibrio spp., general stress response is regulated by sigma factor RpoS that is also responsible for switching the growth to stationary phase (Duval et al., 2015). The entry to the stationary phase requires cooperation of the sigma factor and many other regulators (Llorens et al., 2010). When switching to the stationary phase, growth rate of cells dramatically decreases as a result of reduced protein synthesis. The overall role of RpoS is to ensure adaptation and resistance of the cells to challenging environments. It directly regulates 10% of E. coli genes (Weber et al., 2005) that play role in morphological changes of the cells, resistance to broad range of stresses (oxidative and osmotic stress, heat shock, pH changes, etc.), virulence, metabolic processes, and the GASP (growth advantage in stationary phase) phenotype (Martinez-Garcia et al., 2001; Raiger-Iustman and Ruiz, 2008).

Unlike other Gram-negative bacteria, C. jejuni lacks several stress response genes, including the sigma factor RpoS (Parkhill et al., 2000; Garenaux et al., 2008). Despite its small genome, this strictly microaerobic pathogen had to develop other mechanisms allowing its survival in stressful conditions, such as lack of nutrients in aquatic environments, or high concentration of oxygen when exposed to air or to an oxidative attack of macrophages. The molecular mechanisms responsible for its survival in food, persistence in the environment and virulence have not yet been fully understood. Similarly, no information concerning the transition of C. jejuni cells from exponential to stationary phase are available nowadays. Functional replacement of the sigma factor RpoS has not yet been described and the molecular mechanisms facilitating a cellular switch from exponential to stationary phase remain unknown. Therefore, in this work, proteomic profiles of the cells from exponential and early stationary phase of growth were compared using a 2-D electrophoresis (2DE) and quantitative real-time PCR (qRT-PCR), in order to contribute to better understanding of the molecular changes occurring during cellular transition from exponential to stationary phase.

Materials and Methods

Bacterial Strains and Growth Conditions

In this work, C. jejuni strain 81-176 isolated from a raw milk outbreak (Korlath et al., 1985) was used. Cells were resuscitated from a stock on Karmali agar plates (Oxoid) at 42°C for 48 hours (h) in stainless steel jars filled with gas mixture containing 5% O2, 10% CO2, and 85% N2 (microaerobic atmosphere). Grown cells were subcultured microaerobically in BHI (Merck) for 48 h at 42°C and 110 rpm, and then used for preparation of final suspension. The final suspension was cultivated at 42°C, with shaking at 110 rpm in microaerobic atmosphere for 7 h to harvest proteins in exponential phase and for 18 h for stationary phase. All experiments were performed in 3 biological replicates.

Protein Isolation Purification

Proteins were extracted and purified as described previously (Bieche et al., 2012; Haddad et al., 2012). Briefly, cells were harvested by centrifugation at 7000 g, 4°C for 20 min and washed consecutively with 200 mM glycine (Sigma-Aldrich) and 100 mM Tris-HCl pH 7.0 (Sigma-Aldrich). Pellets were resuspended in 10 ml of a 10 mM Tris-HCl pH 7.0 and the cells were disrupted by series of 6 × 30 s sonication at 20 kHz with 6 min intervals on ice (Vibracell 72434, Bioblock Scientific). Cell debris was removed by two consecutive centrifugations at 10 000 g, 4°C for 20 min. Then, cytoplasmic proteins were separated from membrane fractions by ultracentrifugation at 188 000 g for 1 h at 4°C. The cytoplasmic protein fraction in the supernatant was treated with protease inhibitor cocktail tablets COMPLETE (Roche Diagnostics) and nuclease solution with final concentrations of 18 mg/ml RNAse and 9 mg/ml DNAse (Sigma-Aldrich). Protein samples were dialyzed using cellulose membrane tubing with a cut-off at 12 kDa (Sigma-Aldrich) against MilliQ water at 4°C with shaking for 3 days by refreshing the dialysis bath each day. Total protein concentration was determined using the Micro BCA™ Protein Assay Kit (Perbio-Science).

Two-Dimensional Gel Electrophoresis

A quantity of 100 μg of proteins isolated after 7 and 18 h of cultivation was concentrated using Concentrator 5301 (Eppendorf, France) at room temperature. Samples were mixed with rehydration buffer containing 6 M urea, 2 M thiourea, 4% CHAPS, 0,4% dithiothreitol (DTT), and 2% Bio-Lyse 3/10 Ampholyte (Bio-Rad) and few grains of bromophenol blue (BB). Proteins were then loaded into 17 cm IPG strips (pH 4-7, Bio-Rad) by active rehydration at 50 V for 12 h. After that, the isoelectric focusing (IEF) was performed using the Bio-Rad IEF program as follows: from 50 to 250 V for 3 h, from 250 to 6,000 V for 3 h and at 6,000 V until reaching 54,000 Vh. Strips were then equilibrated in migration buffer containing 6 M urea, 50 mM Tris-HCl (pH 8.8), 2% SDS, 30% glycerol, 2% DTT, 4% iodoacetamide, and few grains of BB. The second dimension was performed using SDS-PAGE with 12% acrylamide gels. The IPG strips were immobilized to the gel using 1% low-melting point agarose (Bio-Rad). The migration ran at 40 mA/gel using Protean II xi cell (Bio-Rad) at 14°C, until the bromophenol blue reached the base of the gels. Proteins in gels were finally silver stained and scanned with a GS-800 densitometer operated with the QuantityOne® software (Bio-Rad). Three independent experiments were performed, with two technical replicates for each of them.

Statistical Analysis and Protein Identification

The image analysis was performed using the Progenesis Samespots® software (NonLinear Dynamics). For the statistical analysis of the results, three independent experiments were performed, each with two technical replicates. Differences between the two conditions from the independent experiments and replicates were validated by Principal Component Analysis (PCA) and differences among matched spot intensities were statistically validated by ANOVA test at a 5% significance level. Lower and higher abundant proteins were taken into account only if the mean difference in spot intensities passed the threshold of 1.5. Predominant proteins whose amount differed significantly between the phases (q- and p-value ≤ 0.05, power ≥ 0.8, fold difference of spot intensities ≥ 1.5) were excised from BioSafe colloidal Coomassie blue (Bio-Rad) stained gels containing in total 700 μg of proteins. The excised proteins were analyzed after trypsin digestion with a mass spectrometer MALDI-TOF Voyager DE super STR (Applied Biosystems) at the PAPPSO platform of the INRA Center in Jouy-en-Josas (France). Gel plugs were first washed twice with 50% (v/v) acetonitrile, 25 mM ammonium carbonate in water, and then dried in a vacuum speed concentrator. Aliquot (10 μl) of a trypsin solution (Promega, 12.5 ng/μl in 50 mM NH4HCO3) was added to each sample and digestion was performed for 6 h at 37°C. A 1 μl aliquot of each supernatant was spotted directly onto the MALDI plate then dried at room temperature before adding 1 μl of the matrix solution (α-cyano-4-hydroxycinnamic acid, 3 mg/ml in 50% (v/v) acetonitrile 0.1% (v/v) trifluororacetic acid). Mass spectra were acquired on a Voyager-DE-STR (Applied Biosystems, Framingham, MA), equipped with a nitrogen laser (Laser Science, Franklin, MA). Tryptic autodigestion ion peaks, (M + H)+ = 2211.104 and 842.509 Da, were used for internal calibration of spectra. The peptide mass lists were used for protein identification in a C. jejuni, strain 81-176, amino acid sequence database (UniprotKB, 09.09.2010), using the Protein Prospector v 3.2.1 software set with the following parameters: mass tolerance <20 ppm, four peptides required to match, one missed cleavage, oxidized methionine and carbamidomethylated cysteine, respectively as possible and fixed modifications. Identification was evaluated with regard to sequence coverage, Mowse score, and compatibility between theoretical and experimental molecular weight and isoelectric point. Genes of proteins significantly over-expressed in one of the growth phases were then chosen for qRT-PCR experiments to verify their expression on translational level.

RNA Isolation and Reverse Transcription

Cells were harvested from cultures cultivated for 4, 7, 12, 16, 18, and 24 h by centrifugation at 3,300 g at 4°C for 6 min, pellets were homogenized with 1 ml of lysis solution Extract-All (Eurobio) according to manufacturer's guidelines. After that, 200 μl of chloroform were added to the suspension allowing separation of cell components. Water phase containing RNA was removed, precipitated with 500 μl of isopropanol and dissolved again in 50 μl of RNase-free water after washing in 75% cool ethanol. Samples were then treated with DNase I (Sigma-Aldrich) and purified using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Possible DNA contamination was detected by PCR detecting house-keeping gene flaA (Nachamkin et al., 1993). The integrity of RNA was verified using the Experion System (Bio-Rad). Its concentration and purity was measured using NanoDrop 2,000 (Thermo Scientific).

For reverse transcription 100 ng of RNA were mixed with 0.5 μl of 1 μM Random Hexamer Primer (Promega) and 4.5 μl of RNase-free water. This mixture was incubated 5 min at 70°C followed by 5 min on ice. After that 15 μl of Master Mix were added to mixture (5 μl of 5 × RT buffer, 1 μl 25 mM dNTP and 1 μl of M-MLV H reverse transcriptase; Promega) and it was incubated 10 min at room temperature, 50 min at 48°C and finally 15 min at 70°C to stop the reaction.

Quantitative PCR (q-PCR)

Quantitative real-time PCR assay was performed using the AB7300 Realtime PCR system with SYBR Green-I Master Mix (Applied Biosystems). Primers (Eurobio) were designed with the on-line Primer3 software (www.simgene.com; Table 1) and their quality was checked both virtually, using FastPCR software, and by PCR. The efficiency of the primer pairs was calculated with the formula E = [10(1/−s)] × 100, where “s” is the slope of the standard curve. The efficiency of designed primers ranged from 101.27 to 105.38%.

TABLE 1
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Table 1. Primers used in the experiments.

The composition of q-PCR mix and amplification program were the same as described by Bieche et al. (2012) Amplification efficiency was determined from standard curve of serially diluted cDNA. The expression level of examined genes was calculated with AB 7,300 software against endogenous control gene rrs to obtain relative quantity.

Purification of Recombinant CosR

In order to produce sufficient amount of His6-tagged recombinant CosR (rCosR), restriction enzymes sites NdeI and BamHI were added to cosR sequence during its amplification using specific primers NtagCosR-NdeI (5'-GCACACATATGAGAATTTTAGTTATAGAAG-3′) and NtagCosR-BamHI (5′-TACAGGATCCAAGGTGCAAAATTGTTA-3′), to obtain NdeI-CosR-BamHI amplicons. These amplicons were inserted using NdeI and BamHI enzymes and subcloned into plasmid pET-15b (Novagen, France) to obtain a N-terminal His6 tagged protein under the control of T7 promoter. The resulting plasmid was transformed to competent E. coli expression strain BL21(DE3) (Novagen, France). Overexpression of rCosR was obtained from an overnight culture at 28°C in LB supplemented with 50 μg/ml of ampicillin, and 0.125 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG). Cells were then harvested by centrifugation at 5,000 g for 10 min, washed with TE buffer (Sigma-Aldrich, France) and resuspended for 30 min in a lysis buffer containing 50 mM TrisHCl, pH 7.5, 300 mM NaCl, 5 mM β-mercaptoethanol and 1 mg/ml of lysozyme. The lysate was centrifuged and rCosR was purified using Ni-NTA agarose columns (Qiagen, France) according to the manufacturer's instructions. Columns were equilibrated using lysis buffer before protein binding and then washed sequentially with buffer W1 (lysis solution with 1% glycerol, 0.5% triton and 20 mM imidazole) and buffer W2 (lysis buffer with 20 mM imidazole). The recombinant protein was eluted using 400 mM imidazole and 6 M urea and purified by dialysis according to the manufacturer's instructions. Aliquots of rCosR were stored at −80°C.

Bioinformatic Analysis of the CosR DNA-Binding Box

A bioinformatic analysis was performed to specify the consensus sequence of the CosR DNA-binding box proposed by Hwang et al. (2011, 2012). For this purpose, sequences of 5′ 400-nt upstream regions of 7 genes previously reported as binding CosR were retrieved from the platform MicroScope (Microbial Genome Annotation & Analysis Platform; http://www.genoscope.cns.fr/agc/microscope/home/). To inspect the conservation of the CosR binding box, sequences of nine different fully sequenced C. jejuni strains were used in the analysis (Supplementary Table 1). Resulting consensus sequence logos were created using the WebLogo platform (http://weblogo.threeplusone.com; Crooks et al., 2004).

Electrophoretic Mobility Shift Assay (EMSA)

EMSA was carried out to identify putative CosR binding sites. DNA fragments covering promoter regulatory regions or gene internal regions of cosR and katA were PCR amplified using designed primers (Table 1). To demonstrate the DNA-binding properties of CosR, concentration range of purified recombinant protein CosR-His6 (1–10 μmol/l) were mixed with 2 μmol of purified PCR fragments in a total volume of 20 μl containing 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 5 mM MgCl2, 10 mM KCl, 10 μg/ml sheared salmon sperm DNA, and 10% glycerol. Sonicated salmon sperm DNA was applied as a nonspecific competitor. All samples were incubated at room temperature for 30 min following by the separation of the complexes using electrophoresis on a native 6% polyacrylamide gel in 0.5 × TBE.

Results

Considering the lack of information concerning the transition of C. jejuni from exponential to stationary phase, we compared the proteomic profiles of cells harvested after 7 and 18 h of microaerobic cultivation at 42°C.

The comparison of the 2-DE proteomic profiles revealed numerous differences between the two phases (Figure 1). In total, 42 spots were identified as 24 different proteins with 11 proteins more abundant in the exponential phase (17 spots), 10 proteins more abundant in the stationary phase (12 spots) and 3 proteins whose isoforms were overexpressed in both exponential and stationary phase (13 spots; Table 2). The identified proteins are involved mostly in metabolism (AcnB, BioB, FumC, HisD, MetK, and OorA), general and oxidative stress response (BetA, ClpB, CosR, DnaK, GroEL, Rbr, and TrxB), translation (FusA, RplL, Tig, Tsf, Tuf, and TypA), and motility (CheW and FlaA).

FIGURE 1
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Figure 1. 2D electrophoretic profiles of C. jejuni proteins isolated after 7 h (exponential phase) and 16 h (early stationary phase) with highlighted overexpressed proteins. A total of 700 μg of cytosolic proteins were separated on IPG strip (pH 4-7) followed by second separation by SDS-PAGE in 12% acrylamide gel stained by Coomassie blue.

TABLE 2
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Table 2. Identification of spots differently expressed in exponential phase (7 h) and early stationary phase (16 h) of C. jejuni growth.

Subsequently, 10 differently expressed proteins were selected for qRT-PCR analysis according to their potential to play role in the phase switch. The selected proteins participate in pathways important for the survival of C. jejuni, such as carbon metabolism (AcnB, FumC, and OorA), general and oxidative stress response (BetA, DnaK, Rbr, and TrxB), motility (FlaA, CheW) and gene expression regulation represented by the essential two-component regulator CosR. When comparing the level of the transcripts, 6 genes were more transcribed in exponential phase (betA, cheW, cosR, fumC, oorA, and trxB), while 3 genes were more transcribed in stationary phase (acnB, flaA, and rbr). There was no difference in the expression of dnaK (Figure 2). In most of the cases, the level of transcription corresponded with the level of protein expression. However, in three cases opposite trends were found between the 2-DE and qRT-PCR data, where the proteins were found to be overexpressed in the stationary phase, while the amount of mRNA was higher in the exponential phase. Those include OorA subunit of enzyme involved in carbon metabolism, CheW protein regulating chemotaxis, and regulator CosR.

FIGURE 2
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Figure 2. Transcript levels of selected genes in early stationary phase (16 h) of C. jejuni growth normalized to the level of transcript from exponential phase (7 h).

As the pleiotropic regulator CosR is essential for C. jejuni growth and regulates several pathways, CosR was selected for further analysis. To evaluate the changes of cosR transcript level throughout the growth of C. jejuni, total mRNA was extracted from the cells at different time-points of their cultivation and cosR specific transcript level was analyzed using qRT-PCR. The amount of cosR transcripts evolved during the growth of the strain 81-176 with an overall fold difference of 5.3. The highest level detected in cells was reached at 7 h of cultivation during the exponential phase and declined constantly afterwards in the stationary phase (Figure 3).

FIGURE 3
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Figure 3. The dynamics of cosR transcript level throughout the growth of C. jejuni normalized to the transcript level at 4 h.

The comparison of the data obtained by qRT-PCR with the 4.0-fold protein increase in the early stationary phase led us to a hypothesis that CosR could undergo a negative autoregulation by binding to its own promoter region. The hypothesis was strengthened by identification of the CosR box described by Hwang et al. (2011, 2012) in C. jejuni 81-176 genome upstream from the cosR gene. Interestingly, the sequences of the CosR-binding boxes of 4 genes responsible for oxidative stress response are conserved among the analysed strains (Figure 4A) with the consensus sequence 5′-wwhhwwwwAAAwTTAdwwTTT-3′ which is similar to the one described by Hwang et al. (2012). To further refine the consensus sequence of the CosR box, 400-nt upstream sequences of the 6 genes previously described as binding CosR (ahpC, cmeA dps, katA, luxS, and sodB) by Hwang et al. (2011, 2012) and the one of CosR itself were compared among 9 fully sequenced C. jejuni strains (Supplementary Table 1). The refine CosR box resulted in the consensus sequence 5′-wdnnhdwnwhwwTTwnhhTTd-3′ (Figure 4B). In order to verify whether CosR can bind to its own promoter region, EMSA was performed (Figure 5). Regions containing the promoter region of cosR and an internal part of cosR gene were amplified and subjected to a concentration range of purified recombinant rCosR. Promoter region of katA from NCTC 11168 was used as a positive control validating the EMSA assays, as rCosR was previously shown to be able to bind to this DNA promoter region (Hwang et al., 2011). A mobility shift was observed when the DNA fragments of cosR and katA promoters were used, while no motility shift was observed for the internal DNA region of cosR, indicating the potential capacity of CosR to bind to its own promoter.

FIGURE 4
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Figure 4. Promoter sequence alignment. (A) Consensus sequence logo from upstream sequence of the promoter region of genes responsible for ROS scavenging (ahpC, cosR, katA and sodB). (B) Consensus sequence logo of CosR-binding box of the upstream sequences of genes with CosR-binding capacity (ahpC, cmeA, cosR, dps, katA, luxS, and sodB). Both logos are based on sequences from 9 strains listed in Supplementary Table 1. D–A, T or G; H–A, T or C; N–A, T, C or G; W–A or T.

FIGURE 5
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Figure 5. In vitro interactions of rCosR with promoter regions. (A) Titration of a 374-bp cosR promoter DNA with rCosR. (B) Shift mobility of 374-bp cosR promoter DNA of C. jejuni 81–176 and the positive control using a 337-bp katA promoter DNA of C. jejuni NTCTC 11168 performed with 10 μmol/l rCosR. (C) Mobility of the promoter and internal DNA fragments of cosR with (+) or without (−) the addition of 10 μmol/l of the purified rCosR protein. L corresponds to 100 bp molecular weight ladder (NE Biolabs).

Discussion

Campylobacter jejuni is repeatedly reported as the major cause of food-borne bacterial enteritides worldwide, causing severe economic and sociological burden. This generally stress-sensitive bacterium, that supposedly cannot multiply outside a host, is able to survive harsh conditions encountered in the environment (Whiley et al., 2013). Despite the concern of this pathogen, relatively little is known about the molecular mechanisms responsible for its ability to persist in meat processing facilities, on carcasses and in the food environment. Enhanced bacterial resistance to various stresses is often connected to the transition of the cells into different phases of growth. For example in the case of E. coli, cells in stationary phase were found to be more resistant to high hydrostatic pressure, mild heat, oxidative and osmotic stress, and chlorine disinfectants (Kaur et al., 1998; Benito et al., 1999; Cherchi and Gu, 2011). However, Campylobacter cells were reported as generally more resistant in exponential phase of growth, with dynamic emergence of resistant subpopulations in the stationary phase (Kelly et al., 2001, 2003). Molecular mechanisms responsible for the transition between the growth phases have not yet been fully described. Unlike other bacteria, C. jejuni lacks sigma factor RpoS regulating the switch from one phase to another (Parkhill et al., 2000). According to several authors, post-transcriptional regulator CsrA could contribute to modulation of gene expression in C. jejuni in response to growth phase. Indeed, in E. coli and S. enterica Thyphimurium, CsrA participates in the regulation of genes inducible in stationary phase (Romeo, 1998; Lawhon et al., 2003). Similar data were recently reported also for C. jejuni 81-176 where CsrA strongly influences expression of proteins in stationary phase and might therefore compensate the lack of RpoS (Fields et al., 2016). Another potential regulator that might participate in the transition could be the stringent response regulator spoT, as it was previously reported as important for survival of cells in stationary phase (Gaynor et al., 2005). As regulations during phase transition in C. jejuni remain unexplained at the molecular level, proteomic fingerprinting of cells isolated from exponential and stationary phase were compared in this work, to identify the main protein changes occurring between the two main growth phases of C. jejuni 81-176.

The first group of identified proteins is involved in cellular stress response. This group contains mostly proteins participating in oxidative stress and protein folding. Interestingly, there is no clear pattern of phase-depending overexpression of these proteins. However, two out of three chaperons were overexpressed in exponential phase, suggesting higher abundance of misfolded and aggregated proteins in the exponential phase, probably caused by the request of functional de novo proteins for rapid biosynthesis of cellular material during cell multiplication. The overexpression of stress-related proteins is not surprising, as toxic metabolites accumulate during growth in batch cultivation. The presence of toxic metabolites together with starvation occurring in the stationary phase could be the reasons why the cells overexpressed proteins involved in the stress response. The overexpression of stress response proteins, reported previously by Wright et al. (2009), could speed up the adaptation process of C. jejuni when changing the environment. Such adaptation would also require adjustment of metabolism reflecting the changes in the availability of nutrients during the cultivation of the cells. Indeed, in this work, we also observed altered expression of several proteins playing role in C. jejuni metabolism, mostly of those involved in the citric acid cycle (TCA). Asaccharolytic bacterium C. jejuni uses TCA as the main carbon source through gluconeogenesis (Velayudhan and Kelly, 2002). Unlike other bacteria, the TCA cycle of C. jejuni involves enzymes containing oxygen-sensitive Fe-S clusters, such as Oor enzymes (Atack and Kelly, 2009) that are rapidly inactivated after exposure to ambient air (Kendall et al., 2014). Higher abundance of OorA observed in the stationary phase could be explained by successful detoxification of ROS with the help of TrxB and BetA which are among the overexpressed protein in the exponential phase.

Another group of proteins with altered expression between the two growth phases is involved in protein biosynthesis. The proteins overexpressed in the exponential phase were mostly elongation factors responsible for correct synthesis of new peptides. The proteins whose abundance is higher in the stationary phase play role in binding of tRNA to ribosomes and their translocation from A to P site. These results suggest high importance of protein biosynthesis in C. jejuni regardless of the physiological state of the cells, although they are in contrast with the decline of ribosomal proteins in the stationary phase reported previously (Wright et al., 2009).

Interestingly, the cells in stationary phase overexpressed proteins involved in motility (flagellar protein FlaA) and chemotaxis (regulator CheW). Similar overexpression was already reported both for cells in stationary phase (Wosten et al., 2004; Wright et al., 2009; Fields et al., 2016) and in biofilms (Kalmokoff et al., 2006). The overexpression of FlaA in stationary phase is mediated by CsrA (Fields et al., 2016) and could be explained by multifunctionality of C. jejuni flagella. Besides their role in motility, flagellum apparatus works as a type III secretion system (Konkel et al., 2004) and it was found to be crucial for C. jejuni adhesion and biofilm formation (Svensson et al., 2014). In our case the protein regulating chemotaxis was also overexpressed, suggesting that the chemotaxis-driven motility is enhanced in stationary phase, facilitating the migration of cells toward nutrient-rich environment.

The transcript analysis revealed that for most of the tested genes, the level of the transcription was correlated to the variation in protein abundance, indicating a regulation of the corresponding proteins at the transcriptional level. However, in three cases (CosR, CheW, and OorA) the level of transcripts was higher in the exponential phase while the protein abundance was significantly lower as compared to the amount in the stationary phase. Such difference could be explained by post-trancriptional or post-translation modifications (Maier et al., 2009). The translation can be delayed when Shine-Dalgarno sequence of mRNA sequence is not completely complementary to the rRNA sequence (Maier et al., 2009) or when the mRNA undergoes conformational change, e.g., by binding of small metabolites (Nahvi et al., 2004). Another reason can be the involvement of short regulatory sRNA that is influencing stability of mRNA and translation process by targeting or blocking mRNA binding to ribosomes (Maier et al., 2009). In addition, overall number of available ribosomes and half-life of translated proteins also play role in final mRNA-protein ratio. Among the proteins presenting an opposite trend between transcript level and protein amount, only CosR is identified as a DNA regulator. This recently discovered orphan two component system (TCS), whose sequence is conserved among Campylobacter and Helicobacter species, affects transcription of 93 different genes, including 17 genes essential for C. jejuni viability (Hwang et al., 2012). It is involved in the regulation pathway of ROS detoxification (Hwang et al., 2011), efflux pump expression (Hwang et al., 2012), and also influences biofilm formation and maturation of C. jejuni (Oh and Jeon, 2014; Turonova et al., 2015). The cosR gene is present in all C. jejuni strains (Gundogdu et al., 2016). It is an essential gene for C. jejuni with the absence of a consensus catalytic aspartic acid replaced by an asparagine. It shares 60% amino acid identity with Hp1043, a response-regulator element of TCS from Helicobacter pylori (Beier and Frank, 2000; Schar et al., 2005; Stahl and Stintzi, 2011). The interchange of C. jejuni cosR with homologous gene from H. pylori does not impair its functionality, indicating a probable conserved function of the gene throughout strains and closely related bacterial genera (Muller et al., 2007). As proteomics analyses using time-point profiling are restricted, dynamics of transcript levels and protein abundance of CosR were explored.

The results indicated a maximum transcribed amount of cosR after 7 h of cultivation in exponential phase followed by a decline up to 24 h of cultivation, while the abundance of the protein was 4-times higher at the early stationary phase. The transient upregulation of the transcript level and the protein amount during the growth led us to a hypothesis that CosR could potentially undergo negative autoregulation. Negative autoregulation, or negative autogenous regulation, is one of the three common motifs used for bacterial regulation of transcription (Shen-Orr et al., 2002), where transcription factor negatively regulates its own transcription (Savageau, 1974). This type of autoregulation has been previously detected in E. coli, where it concerns over 40% of all transcription factors, including global regulators Crp, Hns, Ihf, and Lrp (Shen-Orr et al., 2002). The kinetics of transcription is generally slow, faster kinetics can be achieved by shortening the lifetime of a product by degradation, which is inconvenient due to metabolic cost (Rosenfeld et al., 2002). As it was proven both mathematically and experimentally, negative autoregulation speeds up the transcription response (Rosenfeld et al., 2002; Shen-Orr et al., 2002). However, autoregulation requires active binding of the transcription factor to the promoter region of the operon. Previous studies of binding of CosR–DNA sequences in upstream regions of some genes revealed the presence of a specific CosR-binding box (Hwang et al., 2011, 2012). The resulting consensus sequence of the box was estimated from sequences of 5 genes in C. jejuni NCTC 11168. The authors considered some of the nucleotides to be identical although they were not present in all cases (4/5 for positions 3, 9, 11, 14, 20, and 21). The only identical nucleotides present in all 5 examined regions were on positions 5, 10, 15, and 19. Our analysis show lower level of similarity of the CosR boxes. When we compare the sequences of genes responsible for ROS scavenging (ahpC, cosR, katA, and sodB), the CosR-binding box is conserved and similar to the one reported by Hwang et al. (2012). This consensus sequence was refined using 63 sequences from upstream promoter regions of all genes previously described to bind CosR and the own promoter region of CosR. The resulting consensus sequence indicated a well conserved motif at the end of the consensus sequence which might be valuable for further analyses of the CosR regulon. To detect whether CosR could potentially bind to its own promoter, DNA shift mobility analysis was used. The data indicated a shift mobility of CosR in presence of its own promoter region sequence, which indicates that CosR is also able to bind its own promoter region. Therefore, it might have a potential to undergo a negative autoregulation. The autoregulation of CosR could be also influenced by the post-transcriptional regulator CsrA, which was recently reported to affect CosR expression (Fields et al., 2016). However, due to broad spectrum of genes under CrsA regulation it is not clear whether the observed difference in CosR expression is caused by direct or indirect regulation. Further analyses are therefore required to decipher a potential role of CsrA in cosR expression. Taken together, these data suggest a potential binding of CosR to its own promoter, although additional analyses are required to confirm the in vivo protein-DNA complexation. Future analyses should focus on this potential complexation of CosR with its own promoter and its impact on gene expression.

In summary, our proteomic data show that the transition from exponential to stationary phase is accompanied with numerous changes in the gene expression. The main functional groups of differently expressed proteins involve those playing role in metabolism, stress response, translation, and motility, suggesting active adaptation of the cells to the environment. Our transcriptomic and proteomic analyses also pinpointed the dynamics of the CosR expression, leading to the hypothesis of negative autoregulation of this pleiotropic regulator. Altogether, we outlined mechanisms that C. jejuni could use during the growth.

Author Contributions

OT conceived the study. OT, NH, MH, and HT designed the experiments. HT and MH performed the experiments, DC performed the protein identification. HT and MH analyzed the results and HT prepared the draft manuscript. NH, JP, and OT contributed to the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The reviewer BM and handling Editor declared their shared affiliation, and the handling Editor states that the process nevertheless met the standards of a fair and objective review.

Acknowledgments

The work was financially supported by Secalim (INRA/Oniris, Nantes, France) and the grant of Czech Ministry of Youth and Education No. 7AMB15FR012–“Project Barrande.” Authors would also like to thank Sandrine Rezé, Laurent Marché their technical support.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/article/10.3389/fmicb.2017.00913/full#supplementary-material

Supplementary Table 1. CosR-binding box sequences of genes used in the bioinformatic analysis.

References

Atack, J. M., and Kelly, D. J. (2009). Oxidative stress in Campylobacter jejuni: responses, resistance and regulation. Future Microbiol. 4, 677–690. doi: 10.2217/fmb.09.44

PubMed Abstract | CrossRef Full Text | Google Scholar

Batz, M. B., Hoffmann, S., and Morris, J. G. (2012). Ranking the disease burden of 14 pathogens in food sources in the United states using attribution data from outbreak investigations and expert elicitation. J. Food Prot. 75, 1278–1291. doi: 10.4315/0362-028X.JFP-11-418

PubMed Abstract | CrossRef Full Text | Google Scholar

Beier, D., and Frank, R. (2000). Molecular characterization of two-component systems of Helicobactcr pylori. J. Bacteriol. 182, 2068–2076. doi: 10.1128/JB.182.8.2068-2076.2000

PubMed Abstract | CrossRef Full Text | Google Scholar

Benito, A., Ventoura, G., Casadei, M., Robinson, T., and Mackey, B. (1999). Variation in resistance of natural isolates of Escherichia coli O157 to high hydrostatic pressure, mild heat, and other stresses. Appl. Environ. Microbiol. 65, 1564–1569.

PubMed Abstract | Google Scholar

Bieche, C., De Lamballerie, M., Chevret, D., Federighi, M., and Tresse, O. (2012). Dynamic proteome changes in Campylobacter jejuni 81-176 after high pressure shock and subsequent recovery. J. Proteomics 75, 1144–1156. doi: 10.1016/j.jprot.2011.10.028

PubMed Abstract | CrossRef Full Text | Google Scholar

Blaser, M. J., and Engberg, J. (2008). “Clinical aspects of Campylobacter jejuni and Campylobacter coli infections,” in Campylobacter, 3rd Edn., eds I. Nachamkin, C. Szymanski, and M. Blaser (Washington, DC: ASM Press), 99–121.

Google Scholar

Bronowski, C., James, C. E., and Winstanley, C. (2014). Role of environmental survival in transmission of Campylobacter jejuni. FEMS Microbiol. Lett. 356, 8–19. doi: 10.1111/1574-6968.12488

PubMed Abstract | CrossRef Full Text | Google Scholar

Cherchi, C., and Gu, A. Z. (2011). Effect of bacterial growth stage on resistance to chlorine disinfection. Water Sci. Technol. 64, 7–13. doi: 10.2166/wst.2011.536

PubMed Abstract | CrossRef Full Text | Google Scholar

Crooks, G. E., Hon, G., Chandonia, J. M., and Brenner, S. E. (2004). WebLogo: a sequence logo generator. Genome Res. 14, 1188–1190. doi: 10.1101/gr.849004

PubMed Abstract | CrossRef Full Text | Google Scholar

Duval, M., Simonetti, A., Caldelari, I., and Marzi, S. (2015). Multiple ways to regulate translation initiation in bacteria: mechanisms, regulatory circuits, dynamics. Biochimie 114C, 18–29. doi: 10.1016/j.biochi.2015.03.007

CrossRef Full Text | Google Scholar

EFSA ECDC (2015). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2014. EFSA J. 13:191. doi: 10.2903/j.efsa.2015.4329

CrossRef Full Text

Fields, J. A., Li, J., Gulbronson, C. J., Hendrixson, D. R., and Thompson, S. A. (2016). Campylobacter jejuni CsrA regulates metabolic and virulence associated proteins and is necessary for mouse colonization. PLoS ONE 11:e0156932. doi: 10.1371/journal.pone.0156932

PubMed Abstract | CrossRef Full Text | Google Scholar

Garenaux, A., Guillou, S., Ermel, G., Wren, B., Federighi, M., and Ritz, M. (2008). Role of the Cj1371 periplasmic protein and the Cj0355c two-component regulator in the Campylobacter jejuni NCTC 11168 response to oxidative stress caused by paraquat. Res. Microbiol. 159, 718–726. doi: 10.1016/j.resmic.2008.08.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Gaynor, E. C., Wells, D. H., Mackichan, J. K., and Falkow, S. (2005). The Campylobacter jejuni stringent response controls specific stress survival and virulence-associated phenotypes. Mol. Microbiol. 56, 8–27. doi: 10.1111/j.1365-2958.2005.04525.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Gibney, K. B., O'toole, J., Sinclair, M., and Leder, K. (2014). Disease burden of selected gastrointestinal pathogens in Australia, 2010. Int. J. Infect. Dis. 28, 176–185. doi: 10.1016/j.ijid.2014.08.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Gundogdu, O., Da Silva, D. T., Mohammad, B., Elmi, A., Wren, B. W., Van Vliet, A. H. M., et al. (2016). The Campylobacter jejuni oxidative stress regulator RrpB is associated with a genomic hypervariable region and altered oxidative stress resistance. Front. Microbiol. 7:2117. doi: 10.3389/fmicb.2016.02117

PubMed Abstract | CrossRef Full Text | Google Scholar

Haddad, N., Tresse, O., Rivoal, K., Chevret, D., Nonglaton, Q., Burns, C. M., et al. (2012). Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni. Front. Cell. Infect. Microbiol. 2:30. doi: 10.3389/fcimb.2012.00030

PubMed Abstract | CrossRef Full Text | Google Scholar

Hwang, S., Kim, M., Ryu, S., and Jeon, B. (2011). Regulation of oxidative stress response by CosR, an essential response regulator in Campylobacter jejuni. PLoS ONE 6:22300. doi: 10.1371/journal.pone.0022300

PubMed Abstract | CrossRef Full Text | Google Scholar

Hwang, S., Zhang, Q. J., Ryu, S., and Jeon, B. (2012). Transcriptional regulation of the CmeABC multidrug efflux pump and the KatA catalase by CosR in Campylobacter jejuni. J. Bacteriol. 194, 6883–6891. doi: 10.1128/JB.01636-12

PubMed Abstract | CrossRef Full Text | Google Scholar

Kalmokoff, M., Lanthier, P., Tremblay, T. L., Foss, M., Lau, P. C., Sanders, G., et al. (2006). Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J. Bacteriol. 188, 4312–4320. doi: 10.1128/JB.01975-05

PubMed Abstract | CrossRef Full Text | Google Scholar

Kaur, J., Ledward, D. A., Park, R. W. A., and Robson, R. L. (1998). Factors affecting the heat resistance of Escherichia coli O157: H7. Lett. Appl. Microbiol. 26, 325–330. doi: 10.1046/j.1472-765X.1998.00339.x

CrossRef Full Text | Google Scholar

Kelly, A. F., Martinez-Rodriguez, A., Bovill, R. A., and Mackey, B. M. (2003). Description of a “Phoenix” phenomenon in the growth of Campylobacter jejuni at temperatures close to the minimum for growth. Appl. Environ. Microbiol. 69, 4975–4978. doi: 10.1128/AEM.69.8.4975-4978.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Kelly, A. F., Park, S. F., Bovill, R., and Mackey, B. M. (2001). Survival of Campylobacter jejuni during stationary phase: evidence for the absence of a phenotypic stationary-phase response. Appl. Environ. Microbiol. 67, 2248–2254. doi: 10.1128/AEM.67.5.2248-2254.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

Kendall, J. J., Barrero-Tobon, A. M., Hendrixson, D. R., and Kelly, D. J. (2014). Hemerythrins in the microaerophilic bacterium Campylobacter jejuni help protect key iron–sulphur cluster enzymes from oxidative damage. Environ. Microbiol. 16, 1105–1121. doi: 10.1111/1462-2920.12341

PubMed Abstract | CrossRef Full Text | Google Scholar

Konkel, M. E., Klena, J. D., Rivera-Amill, V., Monteville, M. R., Biswas, D., Raphael, B., et al. (2004). Secretion of virulence proteins from Campylobacter jejuni is dependent on a functional flagellar export apparatus. J. Bacteriol. 186, 3296–3303. doi: 10.1128/JB.186.11.3296-3303.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Korlath, J. A., Osterholm, M. T., Judy, L. A., Forfang, J. C., and Robinson, R. A. (1985). A point-source outbreak of campylobacteriosis associated with consumption of raw-milk. J. Infect. Dis. 152, 592–596. doi: 10.1093/infdis/152.3.592

PubMed Abstract | CrossRef Full Text | Google Scholar

Lawhon, S. D., Frye, J. G., Suyemoto, M., Porwollik, S., McClelland, M., and Altier, C. (2003). Global regulation by CsrA in Salmonella typhimurium. Mol. Microbiol. 48, 1633–1645. doi: 10.1046/j.1365-2958.2003.03535.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, J. (2009). Novel approaches for Campylobacter control in poultry. Foodborne Pathog. Dis. 6, 755–765. doi: 10.1089/fpd.2008.0247

PubMed Abstract | CrossRef Full Text | Google Scholar

Llorens, J. M. N., Tormo, A., and Martinez-Garcia, E. (2010). Stationary phase in gram-negative bacteria. FEMS Microbiol. Rev. 34, 476–495. doi: 10.1111/j.1574-6976.2010.00213.x

CrossRef Full Text | Google Scholar

Maier, T., Güell, M., and Serrano, L. (2009). Correlation of mRNA and protein in complex biological samples. FEBS Lett. 583, 3966–3973. doi: 10.1016/j.febslet.2009.10.036

PubMed Abstract | CrossRef Full Text | Google Scholar

Mangen, M.-J. J., Bouwknegt, M., Friesema, I. H. M., Haagsma, J. A., Kortbeek, L. M., Tariq, L., et al. (2015). Cost-of-illness and disease burden of food-related pathogens in the Netherlands, 2011. Int. J. Food Microbiol. 196, 84–93. doi: 10.1016/j.ijfoodmicro.2014.11.022

PubMed Abstract | CrossRef Full Text | Google Scholar

Martinez-Garcia, E., Tormo, A., and Navarro-Llorens, J. (2001). Further studies on RpoS in enterobacteria: identification of rpoS in Enterobacter cloacae and Kluyvera cryocrescens. Arch. Microbiol. 175, 395–404. doi: 10.1007/s002030100277

PubMed Abstract | CrossRef Full Text | Google Scholar

Muller, S., Pflock, M., Schar, J., Kennard, S., and Beier, D. (2007). Regulation of expression of atypical orphan response regulators of Helicobacter pylori. Microbiol. Res. 162, 1–14. doi: 10.1016/j.micres.2006.01.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Nachamkin, I. (2002). Chronic effects of Campylobacter infection. Microbes Infect. 4, 399–403. doi: 10.1016/S1286-4579(02)01553-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Nachamkin, I., Bohachick, K., and Patton, C. M. (1993). Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J. Clin. Microbiol. 31, 1531–1536.

PubMed Abstract | Google Scholar

Nahvi, A., Barrick, J. E., and Breaker, R. R. (2004). Coenzyme B-12 riboswitches are widespread genetic control elements in prokaryotes. Nucleic Acids Res. 32, 143–150. doi: 10.1093/nar/gkh167

CrossRef Full Text | Google Scholar

Oh, E., and Jeon, B. (2014). Role of alkyl hydroperoxide reductase (AhpC) in the biofilm formation of Campylobacter jejuni. PLoS ONE 9:e87312. doi: 10.1371/journal.pone.0087312

PubMed Abstract | CrossRef Full Text | Google Scholar

Parkhill, J., Wren, B. W., Mungall, K., Ketley, J. M., Churcher, C., Basham, D., et al. (2000). The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403, 665–668. doi: 10.1038/35001088

PubMed Abstract | CrossRef Full Text | Google Scholar

Pope, J. E., Krizova, A., Garg, A. X., Thiessen-Philbrook, H., and Ouimet, J. A. (2007). Campylobacter reactive arthritis: a systematic review. Semin. Arthritis Rheum. 37, 48–55. doi: 10.1016/j.semarthrit.2006.12.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Raiger-Iustman, L. J., and Ruiz, J. A. (2008). The alternative sigma factor, sigma(S), affects polyhydroxyalkanoate metabolism in Pseudomonas putida. FEMS Microbiol. Lett. 284, 218–224. doi: 10.1111/j.1574-6968.2008.01203.x

CrossRef Full Text | Google Scholar

Rodriguez, G. L. A., Ruigomez, A., and Panes, J. (2006). Acute gastroenteritis is followed by an increased risk of inflammatory bowel disease. Gastroenterology 130, 1588–1594. doi: 10.1053/j.gastro.2006.02.004

CrossRef Full Text | Google Scholar

Romeo, T. (1998). Global regulation by the small RNA-binding protein CsrA and the non-coding RNA molecule CsrB. Mol. Microbiol. 29, 1321–1330. doi: 10.1046/j.1365-2958.1998.01021.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Rosenfeld, N., Elowitz, M. B., and Alon, U. (2002). Negative autoregulation speeds the response times of transcription networks. J. Mol. Biol. 323, 785–793. doi: 10.1016/S0022-2836(02)00994-4

PubMed Abstract | CrossRef Full Text | Google Scholar

Salloway, S., Mermel, L. A., Seamans, M., Aspinall, G. O., Shin, J. E. N., Kurjanczyk, L. A., et al. (1996). Miller-fisher syndrome associated with Campylobacter jejuni bearing lipopolysaccharide molecules that mimic human ganglioside GD3. Infect. Immun. 64, 2945–2949.

PubMed Abstract | Google Scholar

Savageau, M. A. (1974). Comparison of classical and autogenous systems of regulation in inducible operons. Nature 252, 546–549. doi: 10.1038/252546a0

PubMed Abstract | CrossRef Full Text | Google Scholar

Saxena, M., John, B., Mu, M., Van, T. T. H., Taki, A., Coloe, P. J., et al. (2013). Strategies to reduce Campylobacter colonisation in chickens. Procedia Vaccinol. 7, 40–43. doi: 10.1016/j.provac.2013.06.008

CrossRef Full Text | Google Scholar

Schar, J., Sickmann, A., and Beier, D. (2005). Phosphorylation-independent activity of atypical response regulators of Helicobacter pylori. J. Bacteriol. 187, 3100–3109. doi: 10.1128/JB.187.9.3100-3109.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Shen-Orr, S. S., Milo, R., Mangan, S., and Alon, U. (2002). Network motifs in the transcriptional regulation network of Escherichia coli. Nat. Genet. 31, 64–68. doi: 10.1038/ng881

PubMed Abstract | CrossRef Full Text | Google Scholar

Stahl, M., and Stintzi, A. (2011). Identification of essential genes in C. jejuni genome highlights hyper-variable plasticity regions. Funct. Integr. Genomics 11, 241–257. doi: 10.1007/s10142-011-0214-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Svensson, S. L., Pryjma, M., and Gaynor, E. C. (2014). Flagella-mediated adhesion and extracellular DNA release contribute to biofilm formation and stress tolerance of Campylobacter jejuni. PLoS ONE 9:e106063. doi: 10.1371/journal.pone.0106063

PubMed Abstract | CrossRef Full Text | Google Scholar

Turonova, H., Briandet, R., Rodrigues, R., Hernould, M., Hayek, N., Stintzi, A., et al. (2015). Biofilm spatial organization by the emerging pathogen Campylobacter jejuni: comparison between NCTC 11168 and 81-176 strains under microaerobic and oxygen-enriched conditions. Front. Microbiol. 6:709. doi: 10.3389/fmicb.2015.00709

PubMed Abstract | CrossRef Full Text | Google Scholar

Velayudhan, J., and Kelly, D. J. (2002). Analysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase. Microbiology 148, 685–694. doi: 10.1099/00221287-148-3-685

PubMed Abstract | CrossRef Full Text | Google Scholar

Weber, H., Polen, T., Heuveling, J., Wendisch, V. F., and Hengge, R. (2005). Genome-wide analysis of the general stress response network in Escherichia coli: sigma(S)-dependent genes, promoters, and sigma factor selectivity. J. Bacteriol. 187, 1591–1603. doi: 10.1128/JB.187.5.1591-1603.2005

CrossRef Full Text | Google Scholar

Whiley, H., van den Akker, B., Giglio, S., and Bentham, R. (2013). The role of environmental reservoirs in human Campylobacteriosis. Int. J. Environ. Res. Public Health 10, 5886–5907. doi: 10.3390/ijerph10115886

PubMed Abstract | CrossRef Full Text | Google Scholar

Wosten, M., Wagenaar, J. A., and van Putten, J. P. M. (2004). The FlgS/FlgR two-component signal transduction system regulates the fla regulon in Campylobacter jejuni. J. Biol. Chem. 279, 16214–16222. doi: 10.1074/jbc.M400357200

PubMed Abstract | CrossRef Full Text | Google Scholar

Wright, J. A., Grant, A. J., Hurd, D., Harrison, M., Guccione, E. J., Kelly, D. J., et al. (2009). Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch. Microbiology 155, 80–94. doi: 10.1099/mic.0.021790-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: foodborne pathogen, Campylobacter jejuni, growth, exponential phase, stationary phase, CosR, regulation

Citation: Turonova H, Haddad N, Hernould M, Chevret D, Pazlarova J and Tresse O (2017) Profiling of Campylobacter jejuni Proteome in Exponential and Stationary Phase of Growth. Front. Microbiol. 8:913. doi: 10.3389/fmicb.2017.00913

Received: 20 January 2017; Accepted: 04 May 2017;
Published: 18 May 2017.

Edited by:

David Rodriguez-Lazaro, University of Burgos, Spain

Reviewed by:

Beatriz Melero, University of Burgos, Spain
Alberto Quesada, University of Extremadura, Spain

Copyright © 2017 Turonova, Haddad, Hernould, Chevret, Pazlarova and Tresse. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Odile Tresse, odile.tresse@oniris-nantes.fr