Original Research ARTICLE
Silicates Eroded under Simulated Martian Conditions Effectively Kill Bacteria—A Challenge for Life on Mars
- 1Department of Bioscience, Aarhus University, Aarhus, Denmark
- 2Space Microbiology Research Group, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany
- 3Department of Chemistry, Aarhus University, Aarhus, Denmark
- 4Stellar Astrophysics Center, Department of Physics and Astronomy, Aarhus University, Aarhus, Denmark
The habitability of Mars is determined by the physical and chemical environment. The effect of low water availability, temperature, low atmospheric pressure and strong UV radiation has been extensively studied in relation to the survival of microorganisms. In addition to these stress factors, it was recently found that silicates exposed to simulated saltation in a Mars-like atmosphere can lead to a production of reactive oxygen species. Here, we have investigated the stress effect induced by quartz and basalt abraded in Mars-like atmospheres by examining the survivability of the three microbial model organisms Pseudomonas putida, Bacillus subtilis, and Deinococcus radiodurans upon exposure to the abraded silicates. We found that abraded basalt that had not been in contact with oxygen after abrasion killed more than 99% of the vegetative cells while endospores were largely unaffected. Exposure of the basalt samples to oxygen after abrasion led to a significant reduction in the stress effect. Abraded quartz was generally less toxic than abraded basalt. We suggest that the stress effect of abraded silicates may be caused by a production of reactive oxygen species and enhanced by transition metal ions in the basalt leading to hydroxyl radicals through Fenton-like reactions. The low survivability of the usually highly resistant D. radiodurans indicates that the effect of abraded silicates, as is ubiquitous on the Martian surface, would limit the habitability of Mars as well as the risk of forward contamination. Furthermore, the reactivity of abraded silicates could have implications for future manned missions, although the lower effect of abraded silicates exposed to oxygen suggests that the effects would be reduced in human habitats.
An in-depth understanding of the interaction between the Martian surface environment and living cells is essential for assessment of the habitability of Mars, the risk of forward contamination and the hazards associated with manned missions. The Martian surface temperatures vary from −150° to 20°C, and the surface pressure is usually 4–10 mbar (Millour et al., 2015). Consequently, the Martian surface is extremely arid and even if liquid water is present, the water activity is likely too low to support life (Martin-Torres et al., 2015; Ojha et al., 2015). While such an arid, cold, low-pressure environment often is not detrimental for desiccation tolerant, dormant cells and spores, the radiation on the Martian surface can be highly damaging (Hansen et al., 2009; Johnson et al., 2011). The solar irradiation flux on Mars is about 40% of the irradiation on Earth, but, the thin atmosphere and the sparse ozone layer (Lefevre et al., 2008) result in a substantially higher UVB and UVC radiation than on Earth (Cockell et al., 2000). Schuerger et al. (2003) demonstrated that 15 min of exposure to Mars-like UV radiation sterilized monolayers of Bacillus subtilis spores. The effect of UV radiation is, however, reduced dramatically when cells and spores are shielded by a thin layer of dust or a few layers of dead cells (Mancinelli and Klovstad, 2000; Diaz and Schulze-Makuch, 2006; de La Vega et al., 2007; Paulino-Lima et al., 2010). The flux of ionizing radiation from the sun and galactic cosmic rays are three orders of magnitude higher on the Martian surface as compared to the surface of Earth due to the thin Martian atmosphere and the absence of a global magnetic field (Hassler et al., 2014). Nevertheless, ionizing radiation from the sun only poses a minor challenge for B. subtilis and D. radiodurans, and a cover that would protect against UV radiation would also shield from the charged particles in the solar wind (Tuleta et al., 2005; Paulino-Lima et al., 2011). Protection against galactic cosmic rays, mainly high-energy particles, requires much thicker shielding. However, the flux to the Martian surface would only cause a half-life of B. subtilis spores within the order of thousands of years (Moeller et al., 2010; Hassler et al., 2014). In summary, the Martian surface does not generally allow life to proliferate, but may not pose an immediate threat to dormant cells.
The Martian soil is dominated by silicate minerals (Bish et al., 2013) primarily produced by physical abrasion of olivine basalts (Morris et al., 2004; Gunnlaugsson et al., 2009). The soil and dust particles are produced by meteor impacts and by wind-driven saltation through repetitive low-energy collisions (Kok et al., 2012). A recent study showed that silicates exposed to simulated wind-driven saltation in a Mars-like atmosphere could lead to a production of reactive oxygen species (ROS) including hydrogen peroxide (H2O2) and hydroxyl radicals (·OH) (Bak et al., 2017). A production of ROS from abraded silicates may explain the oxidative capabilities of the Martian soil as observed by the Viking Biological Experiments (Klein, 1978) and may cause oxidative stress for living cells. Based on these observations, we hypothesized that wind abraded silicates on Mars may pose an additional stress factor for living cells.
To test our hypothesis, we conducted a series of experiments in which cell suspensions of Pseudomonas putida, B. subtilis and D. radiodurans were exposed to quartz and basalt samples abraded by simulated wind-driven saltation in Mars-like atmospheres. The exposure experiments were conducted without exposing the abraded material to oxygen to simulate the in situ conditions for indigenous organisms and a scenario of forward contamination. Furthermore, we investigated the effect of secondary exposure to air to simulate the environment inside a human habitat. Pseudomonas putida is a common soil bacterium, which can withstand some level of oxidative stress (Kim and Park, 2014). The endospores of B. subtilis and cells of D. radiodurans have been reported to be highly resistant against a range of stress factors including desiccation, chemical oxidative stress (Melly et al., 2002; Slade and Radman, 2011) and ionizing radiation (Anderson et al., 1956; Moeller et al., 2008). Furthermore, they have been used to study the effects of exposure to a Mars-like environment including the impact of UV and ionizing radiation (Diaz and Schulze-Makuch, 2006; de La Vega et al., 2007; Moeller et al., 2010; Kerney and Schuerger, 2011). The resistance of B. subtilis to abraded silicates was further investigated by testing a range of mutant strains lacking specific spore components, which previously have been found to be related to resistance against oxidizing agents (Setlow, 2014). Bacillus sp. spores are of particular interest and concern, as they are common contaminants in cleanrooms used for assembly of spacecraft (Puleo et al., 1977; La Duc et al., 2009; Smith et al., 2017) and thus likely agents of forward contamination.
Materials and Methods
Simulated Saltation of Silicates
Quartz sand samples were prepared by sieving commercially available quartz (Merck, Cat. No. 1.07536) to obtain the 125–1,000 μm fraction. The quartz sand was washed and dried to remove small particles and finally divided into 10 g aliquots using a Fritsch Rotary cone samples divider. The same procedure was used to produce 10 g basalt aliquots based on olivine basalt that was collected in Gufunes on Iceland (64°08′22.18″N, 21°47′21.27″W) and crushed. The mineral composition of the same batch of crushed basalt was reported in Bak et al. (2017). The samples were transferred to about 20 cm long and 3 cm wide quartz ampoules with rounded ends (Figure 1A). One end of the ampoules was extended by a narrow quartz inlet tube that allowed connection to a vacuum system. The ampoules were evacuated to <0.12 mbar and filled with 8 ± 0.2 mbar of a Mars-like atmosphere composed of 95% CO2 (>99.9% purity, AGA, Denmark) and 5% of a custom made gas mixture (>99% purity, Air Liquid, Denmark) to give a final composition of 95% CO2, 3% N2, 1.75% Ar, 0.15% O2 and 0.1% CO, which is similar to the composition of the Martian atmosphere (Mahaffy et al., 2013). The pressure was monitored with a Pfeiffer TPR 265 Pirani gauge for p < 1 mbar and a Pfeiffer APR 250 Pirani gauge for p > 1 mbar. The ampoules were sealed by melting off the narrow inlet tubes. Wind-driven saltation was simulated by mounting the ampoules in a turning wheel running at 30 rpm (Figure 1B). This set-up caused the silicate samples to fall from one end of the ampoules to the other once per second, which mimics the repeated low energy impacts that are characteristic for saltation (Merrison, 2012). The samples were tumbled for 63 days, which led to a considerable abrasion of the material resulting in an increase of the specific surface area of 3.44 and 0.76 m2 g−1 for the quartz and the basalt samples, respectively (Bak et al., 2017). Some of the abraded samples were suspended in 10 ml of water followed by overnight drying at 60°C, which has been shown to neutralize the potential for production of ROS (Bak et al., 2017). These samples were used as negative controls and are hereafter referred to as inactivated samples.
Figure 1. (A) Sealed quartz ampoules with basalt (left) and quartz sand (right). (B) Turning wheel running at 30 rpm. The ampoules were fixed inside the gray boxes. (C) Media inoculated with cell cultures. (D) Abraded silicate samples mixed with washed cell cultures. (E) Agar plates with colonies of the tested bacteria.
Preparation of Cell Suspensions
The bacterial strains used in this study are listed in Table 1. Pseudomonas putida mt-2 KT2440, Bacillus subtilis 168 and Deinococcus radiodurans R1 were obtained from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany) as DSM6125, DSM402 and DSM20539, respectively. Stock cultures of all strains were stored as aliquots in 15% glycerol at -80°C. The following growth media and buffer solutions were used: Lysogeny Broth (LB) medium [10 g NaCl, 10 g tryptone (Fluka) and 5 g yeast extract (Merck) per liter, pH adjusted to 7]; Nutrient Broth (NB) medium (Scharlau) and phosphate buffered saline (PBS) (8 g NaCl, 0.2 g KCl, 1.44 g Na2HPO4 and 0.24 g KH2PO4 per liter, pH adjusted to 7.3). For preparation of agar plates the liquids were supplemented with 1.5% w/w of bacteriological agar. All chemicals were of analytical grade and acquired from commercial suppliers. MilliQ water was used for all solutions. P. putida cultures were grown in 100 ml Erlenmeyer flasks containing 30 ml of medium composed of 20% LB-medium and 80% PBS. Bacillus subtilis and D. radiodurans were grown under the same condition, but in a medium composed of 20% NB-medium and 80% PBS (Figure 1C). Pre-cultures were prepared from frozen stocks and incubated at room temperature (21–23°C) on a shaker at 120 rpm. The optical density (OD) was measured after ~24 h at 600 nm and the equivalent of 1 ml of OD600 = 0.1 was transferred to fresh medium and incubated under identical conditions. The survival experiments were carried out with cultures that were harvested in the late exponential growth phase (after 10–12 h for P. putida and B. subtilis and 17–19 h for D. radiodurans) and in the stationary phase (after 118–120 h for all species), see Figure S1. The purity of the cultures was checked by microscopy.
Prior to the survival tests, the cultures were centrifuged at 4,696 × g for 5 min. in 50 ml sterile Falcon tubes. The supernatant was discarded and the pellet was resuspended in 30 ml PBS. This washing procedure was done twice.
All Bacillus subtilis strains used for the spore resistance experiments were congenic to their respective wild-type strain. Spores were obtained by cultivation under vigorous aeration in double-strength liquid Schaeffer sporulation medium (Schaeffer et al., 1965) under identical conditions for each strain, purified and stored as described by Nicholson and Setlow (1990). When appropriate, chloramphenicol (5 μg/ml), neomycin (10 μg/ml), or tetracycline (10 μg/ml) was added to the medium (Table 1). Spore preparations consisted of single spores with no detectable clumps, and were free (>99%) of growing cells, germinated spores and cell debris, as seen in the phase-contrast microscope (Nagler et al., 2014).
Exposure of Bacteria to Abraded Silicates
The cell and spore suspensions were adjusted to ~106 colony forming units (CFU)/ml in PBS. For the experiments conducted in air to simulate the effect of abraded silicates inside a human habitat, the ampoules were opened by scoring and breaking of the narrow neck and the abraded silicate samples left exposed to ambient air. After 5 min exposure to ambient air, the silicate samples were mixed with the cell suspensions at a mass ratio of 1:2 (Figure 1D). The resulting slurries were vortexed and placed on a shaker at 120 rpm. Control samples were prepared in the same manner by mixing the inactivated silicate samples with the cell suspensions at a 1:2 mass ratio followed by vortexing and shaking. Experiments that were conducted under anoxic conditions to simulate the in situ conditions on the Martian surface were executed in the same way as the samples exposed to air except that the cell suspension were flushed with N2 (>99.9% purity, AGA, Denmark) prior to use to remove dissolved oxygen and that the ampoules and silicate samples were handled in a N2–flushed glove box. A minimum of three samples tumbled in separate ampoules were prepared for each treatment, which forms the basis for the calculated standard error of mean (SEM). All samples were exposed to air when the first subsamples were taken for plating after 15 min.
The initial CFU count for each cell suspension in PBS was determined by plating in triplicates. The number of CFU was determined after 0.25, 1, 2.5, 5, and 24 h for the cell suspensions with the abraded silicates, the corresponding oxic and anoxic PBS controls as well as for the control samples with inactivated silicates. To determine the surviving fraction of cells after a given exposure time, the samples were vortexed and serial dilutions of the subsamples were plated. The survival of D. radiodurans and B. subtilis 168 was evaluated by plating on NB-plates while LB-plates were used to evaluate the survival of P. putida and the other B. subtilis strains. The plates were incubated at 30°C, and the number of colonies was determined after at least 1 day of incubation for P. putida and B. subtilis and after 2 days for D. radiodurans (Figure 1E). The number of colonies did not change considerably after extended incubation time. The surviving fraction was calculated as the mean number of CFU relative to the starting concentration. 100 μl of the cell suspension was streaked onto each plate giving a level of detection (LOD) of 10 CFU ml−1 for undiluted samples. Samples with a surviving fraction below the LOD was plotted as having a CFU count equal to the LOD. For statistical comparison, we performed unpaired t-tests on the log-transformed CFU counts assuming unequal variances. We had a minimum of three biological replicates for each treatment and used a significance threshold of p < 0.05.
Inactivated quartz and basalt samples as well as abraded samples that had not been exposed to water were mixed with PBS at a 1:2 mass ratio in triplicates. The pH was measured using a glass pH electrode (Mettler Toledo Inlab® Expert Pro-ISM) with a Mettler Toledo Seven Compact pH meter after 24 h.
The number of CFU of P. putida harvested during exponential growth increased in the PBS controls as well as in the controls with inactivated silicates (Figure 2A). The abraded silicates caused a significant decrease in the number of CFU as compared to the inactivated controls with an about 99% decrease with quartz and to a concentration below the LOD with basalt (p < 0.001, n ≥ 3). Stationary phase cultures of P. putida cells were not affected by abraded quartz while abraded basalt killed approximately 99% of the cells within 24 h (Figure 2B), and thus caused a significant decrease in the viability as compared to the inactivated basalt (p = 0.008, n ≥ 3).
Figure 2. The percentage of CFU of P. putida harvested in the exponential growth phase (A) and in the stationary phase (B) in a PBS control (—X—) and after addition of abraded quartz () and basalt () relative to the starting concentration of ~106 CFU/ml. The survival of P. putida is shown for experiments with inactivated silicates (orange) and abraded silicates kept anoxic (blue). The error bars show the SEM, and the asterisks indicate that the number of CFU was below the LOD.
Microscopic examination showed that the B. subtilis cultures harvested in the exponential growth phase were dominated by vegetative cells with no endospores observed. B. subtilis harvested in the stationary phase was a mixture of free endospores, endospores encapsulated by mother cells and vegetative cells without endospores. A Bacillus subtilis culture harvested in the stationary phase and heated to 80°C for 10 min showed a decrease to 64 ± 6% of the CFU counts before heating (average ± SEM, n = 3), which is in good agreement with the observed proportion of endospores. Cells of B. subtilis harvested in the exponential growth phase showed a substantial decrease in CFU even in the PBS control (Figure 3A). There was a faster decrease in the number of CFU in samples supplemented with abraded basalt, but the response did not differ significantly from the PBS control after 24 h of exposure (p = 0.70, n ≥ 3). The final concentration of CFU in experiments conducted with stationary phase cultures was 65-85% of the starting concentration for all treatments including the PBS control (Figure 3B).
Figure 3. The percentage of CFU of B. subtilis harvested in the exponential growth phase (A) and in the stationary phase (B) in a PBS control (—X—) and after addition of abraded quartz () and basalt () relative to the starting concentration of ~106 CFU/ml. The minimum and maximum number of CFU in the PBS control replicates are marked by the gray area. The survival of B. subtilis is shown for experiments with inactivated silicates (orange) and abraded silicates kept anoxic (blue). The error bars show the SEM, and the asterisks indicate that the number of CFU was below the LOD.
Most of the B. subtilis wild-type and mutant strains showed a change in the number of CFU of less than 50% of the starting concentration following all treatments (Table 2). The only exception was PS356, which showed a decrease in the number of CFU for all treatments. This indicates that while small acid-soluble spore proteins (SASP) do not seem to be important for the resistance toward abraded basalt, they are generally important for spore survival. None of the strains had a significantly lower number of CFU after exposure to basalt as compared to both the PBS and the inactivated basalt controls (p ≥ 0.06, n = 3, for all strains).
Table 2. The percentage of CFU of B. subtilis spores relative to the starting concentration following exposure to PBS, inactivated basalt and basalt for 24 h.
The number of D. radiodurans CFU grown from exponential growth phase cells did not change significantly in the PBS and the quartz samples within 24 h of exposure (Figure 4A) (p ≥ 0.14, n ≥ 4). Samples exposed to abraded basalt showed the same trend as P. putida with an increase in the CFU number in inactivated samples and a decrease to below the LOD in activated samples (p < 0.001, n ≥ 3). Inactivated basalt did not have a considerable effect on the number of CFU of stationary phase cells while activated basalt led to a decrease in CFU number to below the LOD, although slower than for cells in the exponential growth phase (Figure 4B).
Figure 4. The survival of D. radiodurans harvested in the exponential growth phase (A) and in the stationary phase (B) in the PBS control (—X—) and after addition of abraded quartz () and basalt () relative to the starting concentration of ~106 CFU/ml. The survival of D. radiodurans is shown for experiments with inactivated silicates (orange) and abraded silicates kept anoxic (blue). The error bars show the SEM, and the asterisks indicate that the number of CFU was below the LOD.
Silicate samples exposed to oxygen following the abrasion showed a lower effect on the viability of P. putida and D. radiodurans as compared to the effects of samples kept anoxic, with the exception of D. radiodurans harvested in the exponential growth phase and exposed to abraded quartz (Table 3). The CFU counts were in general lower than the CFU counts obtained from corresponding inactivated samples, but never decreased to below the LOD.
Table 3. The relative number of CFU 24 h after addition of abraded quartz or basalt (average % of initial concentration ± SEM).
Addition of abraded quartz to PBS did not affect the initial pH of 7.3 ± 0.1 while addition of abraded basalt led to an increase in pH to 8.2 ± 0.1. The inactivated controls showed the same pH effect as the abraded silicates that had not been exposed to water.
Cells exposed to abraded silicates experience a range of potential stresses including anoxia, starvation, pH changes and ROS. The combined effect of these factors had a detrimental effect on vegetative cells as clearly seen by the experiments with abraded basalt. By including PBS controls with each experiment, we could isolate the effect of starvation and anoxia. Changes in the number of CFU due to adhesion of cells to the silicate particles or growth stimulated by supply of micronutrient should depend on the mineral composition and thus be independent of whether the samples had been inactivated or not. The inactivated silicate controls could therefore be used to account for potential effects of adhesion, supply of micronutrients as well as pH changes, which were found to be unaffected by the inactivation process. Thus, by comparing the effects of abraded silicates to the corresponding inactivated controls, we could elucidate the effects of ROS produced by the abraded silicates as well as possible direct effects of reactive surface sites produced during abrasion.
Viability of Bacteria in PBS and Inactivated Silicate Controls
Each of the PBS controls shown in Figures 2–4 present the average of five cell cultures of which some were prepared in oxic PBS and some were prepared in anoxic PBS. We did not observe any effect of the presence of oxygen in the PBS controls and thus conclude that the oxygen regime did not directly affect the survival of the cells. Starvation, as it was induced by transferring cells from nutrient rich growth medium to PBS, affected the three bacterial species in different ways.
The increased number of CFU for exponential growth phase P. putida cells in PBS and in suspensions with inactivated silicate samples could partly be explained by cell division combined with a reduction in cell size. This is supported by a reduced average forward scatter and SYTO9 intensity over time (Figure S2), which reflect the overall cell size and the DNA content, respectively. Furthermore, Pseudomonas putida KT2440, which is identical to P. putida mt-2 with the exception of the presence of the TOL plasmid in mt-2 (Nakazawa, 2002), is known to accumulate large amount of polyhydroxyalkanoate (PHA) (Follonier et al., 2011). Accumulated PHA by P. putida mt-2 in our experiments could have provided an energy and carbon source for the additional cell divisions. The large increase in CFU number after addition of abraded basalt could indicate that growth was further enhanced by micronutrients supplied by the basalt. However, the increase in the number of CFU in the samples with inactivated quartz as compared to the PBS control remains unexplained.
The number of CFU of B. subtilis harvested in the exponential growth phase and transferred to PBS ranged from below the LOD to about 2% of the starting concentration after 24 h (Figure 3A). Microscopic inspection of the cells revealed that the B. subtilis cells became motile toward the end of the exponential growth phase and started to form endospores at the transition to the stationary phase. Bacillus subtilis 168 has not been shown to produce PHA (Singh et al., 2009) and, while some strains of B. subtilis can accumulate glycogen during sporulation with media containing e.g. xylose, (Kiel et al., 1994), there are no indications that Bacillus subtilis 168 would have accumulated storage compounds in our experiments. The sequential response to nutrient limitation with transformation into a motile stage followed by sporulation in combination with a lack of energy reserves and a sudden change to nutrient-free PBS may explain the lethal effect of starvation. The varying number of CFU after 24 h could be the result of a small but variable number of endospores in the cell suspension.
The moderate increase in the number of CFU of D. radiodurans harvested in the exponential growth phase in the inactivated silicate control samples could be due to ongoing cell division and/or separation of cell aggregates.
The Stress Effect of Abraded Quartz
Abraded quartz did not have a considerable effect on the survival of cells harvested in the stationary phase but killed up to 91% of P. putida cells harvested during exponential growth (Table 3). Previous studies have shown that the addition of abraded quartz would have caused a release of about 9 μM ·OH and 31 μM H2O2 (Bak et al., 2017). This ROS production may be the cause of the observed effect as H2O2 can damage cells directly by e.g., oxidation of [4Fe-4S] clusters of dehydratases (Jang and Imlay, 2007) and mononuclear iron enzymes (Anjem and Imlay, 2012) and indirectly by production of ·OH by reactions with transition metal ions through Fenton (i.e., H2O2 + Fe2+ → OH− + ·OH + Fe3+) and Fenton-like reactions (Prousek, 2007). Hydroxyl radicals react with most organic compounds at diffusion limited rates (Buxton et al., 1988) and can lead to e.g., DNA damage (Bjelland and Seeberg, 2003) and damage of cell membranes by initiating lipid peroxidation (Buege and Aust, 1978).
The bacteria used in our assays are accustomed to some level of oxidative stress as ROS constantly are produced intracellularly by adventitious reduction of molecular oxygen by reduced redox enzymes (Seaver and Imlay, 2004; Korshunov and Imlay, 2010). For E. coli it has been shown that these processes lead to an intracellular H2O2 concentration of about 50 nM H2O2 (Imlay, 2013) and that the production rate is equal to the influx of H2O2 when extracellular H2O2 concentrations were about 200 nM (Seaver and Imlay, 2001). An extracellular concentration of about 31 μM H2O2 following addition of abraded quartz would thus significantly increase the oxidative stress inside the cells. The increased oxidative stress would, however, not necessarily result in reduced survival as P. putida, B. subtilis as well as D. radiodurans possess a range of detoxification mechanisms.
Our test organisms can produce catalases and peroxidases and thereby effectively decompose H2O2 (Inaoka et al., 1999; Slade and Radman, 2011; Kim and Park, 2014; Svenningsen et al., 2015). Furthermore, all three species possess manganese and manganese complexes which increase the resistance to oxidative stress by scavenging and H2O2 and thus counteract protein damage (Inaoka et al., 1999; Horsburgh et al., 2002; Daly et al., 2010; Banh et al., 2013). Manganese can also replace iron in mononuclear enzymes (Anjem et al., 2009) which neutralizes this potential cause of Fenton-like reactions (Anjem and Imlay, 2012). Especially D. radiodurans is known to accumulate high concentrations of manganese, which is linked to their extreme radiation resistance (Daly et al., 2004, 2010). Also, Deinococcus radiodurans cells contain carotenoids, which function as antioxidants (Tian et al., 2007).
Despite this array of defense mechanisms, the cells may acquire DNA damage by oxidative stress. The vegetative cells of P. putida, B. subtilis, and D. radiodurans can counteract DNA damage by an array of DNA repair mechanisms (Slade and Radman, 2011; Lenhart et al., 2012; Mielecki et al., 2013) and especially D. radiodurans can withstand high degrees of DNA damage due to effective protection of the DNA repair proteins by manganese complexes (Daly et al., 2010). Even though B. subtilis endospores do not actively maintain the DNA while dormant, they are highly resistant to H2O2 due to the combined effect of shielding by the spore coat (Riesenman and Nicholson, 2000), low permeability of the compact inner membrane (Cortezzo and Setlow, 2005) and stabilization of DNA by α/β-type small acid soluble spore proteins (Setlow and Setlow, 1993) and dipicolinic acid (Setlow et al., 2006).
It has been shown that P. putida cells can survive exposure to 4 and 50 mM H2O2 in exponential phase and stationary phase, respectively (Klotz and Anderson, 1994) and the viability of D. radiodurans is unaffected by exposure to up to 10 mM H2O2 (Tian et al., 2007). Likewise, vegetative cells of B. subtilis are not affected by addition of 100 μM H2O2 (Hartford and Dowds, 1994) and endospores can resist up to 1.5 M H2O2 (Melly et al., 2002). As the expected H2O2 production from the abraded silicates is orders of magnitude lower than the H2O2 concentrations previously shown to affect the viability of P. putida, the observed effect on survival cannot be explained by H2O2 release. The production of ·OH from abraded quartz, however, may be responsible for the observed effects. The high reactivity of ·OH toward most organic compounds makes it virtually impossible for the cells to counteract the direct effects of ·OH.
The Stress Effect of Abraded Basalt
Exposure to abraded basalt effectively killed both exponential growth and stationary phase cells of P. putida and D. radiodurans (Figures 2, 4). The low effect of the corresponding inactivated control samples suggests that the low viability is caused by a production of ROS from the abraded basalt samples. In a previous study, basalt samples did not lead to production of H2O2 in water, which is surprising as a fraction of the abraded material originated from abrasion of the quartz ampoules (Bak et al., 2017). It was suggested that the absence of H2O2 may be due to the presence of transition metal ions e.g., iron in the basalt, which could catalyze the breakdown of H2O2. This would entail a production of highly reactive ·OH, which may explain the greater toxicity of basalt. The significantly lesser effect of abraded basalt exposed to oxygen (Table 3) supports this hypothesis as e.g., Fe2+ could become oxidized by oxygen, which would counteract Fenton reactions.
Spore resistance of B. subtilis to abraded silicates could not be attributed to any specific spore components, as the tested mutants in general showed the same high level of resistance to abraded basalt as the wild-type strains. Based on previous studies it has been suggested that the resistance of B. subtilis spores to oxidizing agents is related to enzymes in the outer layers, spore coat proteins, low permeability of the compact, inner membrane and the presence of α- and β-SASPs (Setlow, 2014). While we have tested the role of the spore crust, the spore coat and α- and β-SASPs individually, we cannot exclude that the resistance is due to a combination of a range of factors for which none of the tested components are essential. If ·OH was the main agent for oxidative damage caused by abraded basalt, then lipid peroxidation could be the main cause of the effects observed with vegetative cells. In this case, we would expect the spore resistance of B. subtilis to be at least partly attributed to the spore layers shielding the cell membranes. This was partly tested with the mutant lacking the spore crust and the mutants lacking either the inner or the outer or both layers of the spore coat (Table 2). These mutants did, however, all maintain some parts of the outer layers of the spore, which may have been sufficient to shield the outer membrane.
The Stress Effect of Wind-Abraded Silicates on Mars
Our results show that the toxicity of abraded silicates is strongly influenced by the mineralogy and secondary exposure to oxygen. The Martian soil is mainly composed of basaltic material of which the crystalline fraction is dominated by plagioclase feldspar, olivine and augite (Bish et al., 2013). This is similar to the composition of the basaltic material used in this study (Bak et al., 2017). Furthermore, under Martian in situ conditions the abraded basalt would only be exposed to the 10−5 bar oxygen present in the Martian atmosphere, which equals the oxygen concentration used for the simulated Martian atmosphere. Thus, abraded basalt that was not secondarily exposed to oxygen is the most realistic analog of the Martian soil investigated in this study. Interestingly, these samples also showed the strongest detrimental effect on survival of our test organisms.
Our exposure experiments were conducted in aqueous solution. Therefore, cells exposed to Martian soil as a result of e.g., forward contamination would not initially be exposed to the effects examined here. All known lifeforms do, however, require water for metabolic activity and ultimately to proliferate, in which case the stress effects of abraded silicates would apply. This could be circumvented by e.g., B. subtilis which in the form of endospores were largely unaffected by abraded silicates. Upon initial exposure to water the endospore would not be affected by the abraded silicates, and at the time of sporulation we would expect a much lower stress effect, if any, as shown by our experiments with inactivated basalt. Recent studies suggest annual formation of thin films of water on Mars at the northern water ice annulus (Kereszturi and Appere, 2014) and possibly more widespread night-time transient liquid brines caused by uptake of water vapor by deliquescent salts (perchlorates) in the soil (Martin-Torres et al., 2015). It is possible that reactive, abraded silicates accumulate in dry periods and are allowed to react periodically and locally when water is available. Abraded silicates have been found to form covalent bonds with atmospheric methane in a dry state (Jensen et al., 2014; Bak et al., 2016). Similar reactions may occur between abraded silicates and cells in a dry state, which could pose an additional challenge for life. This, however, was not investigated in the present study and awaits therefore further exploration.
The reactivity of the Martian soil is also pertinent in relation to the risk of manned missions. Freshly produced silicate dust has for long been known to cause serious health problems within the mining industry (Fubini and Hubbard, 2003), and during the Apollo missions the astronauts were complaining about respiratory irritation due to inhalation of Lunar dust brought into the spacecraft (Cain, 2010). This is partly related to physical irritation caused by inhalation of particles, but also due to production of ROS (Fubini and Hubbard, 2003; Wallace et al., 2009). The results of our experiments indicate that the reactivity of abraded silicates could be even more problematic in the dry, almost anoxic atmosphere of Mars. However, this stress effect would likely be lower inside a human habitat as secondary exposure to air led to a considerable decrease in toxicity. As shown with inactivated basalt, the effect was further reduced by exposing the abraded material to water, which could be used as simple detoxification measures.
The dramatic effects of abraded basalt on the survival of P. putida, and the highly radiation resistant D. radiodurans suggest that the Martian surface is even more hostile to terrestrial organisms than previously thought. This should lower the risk of forward contamination but may also add to the hazards encountered by future manned missions. We have not determined the cause(s) behind the toxicity of abraded silicates but put forward the hypothesis that the toxicity is the result of a production of H2O2, which through Fenton-like reactions facilitated by transition metal ions in the basalt led to the formation of highly reactive ·OH. We propose that organisms isolated from spacecraft assembly clean rooms should be examined with respect to their resistance toward exposure to abraded silicates for a better assessment of the risk of forward contamination.
The study was conceived by EB, SJ, PN, and KF. EB and LJ made a pilot study for the survival assay. The experiments with B. subtilis mutant strains were conducted by ML and RM and the rest of the survival experiments were performed by EB. SN conducted the flow cytometry analyses. The manuscript was written by EB and KF with input from ML, SN, LJ, RM, SJ, and PN.
This research was financially supported by the Danish Council for Independent Research, Natural Sciences (ref. 09-066733). RM was supported by DLR grant DLR-FuE-Projekt ISS LIFE, Programm RF-FuW, Teilprogramm 475.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
We would like to thank J. J. Iversen for construction of the tumbling system, J. C. Kondrup for making the ampoules and B. Rasmussen for preparation of the quartz and basalt samples. The authors thank Andrea Schröder for her technical assistance during parts of this work and acknowledge Peter Setlow and Patrick Eichenberger for their generous donation of the Bacillus subtilis mutant strains. Also, we will like to thank Nanna Svenningsen for providing the Pseudomonas putida culture. Flow cytometry was performed at the FACS Core Facility, Aarhus University, Denmark.
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/article/10.3389/fmicb.2017.01709/full#supplementary-material
Anderson, A. W., Nordan, H. C., Cain, R. F., Parrish, G., and Duggan, D. (1956). Studies on a radio-resistant micrococcus. 1. Isolation, morphology, cultural characteristics, and resistance to gamme radiation. Food Technol. 10, 575–578.
Anjem, A., Varghese, S., and Imlay, J. A. (2009). Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli. Mol. Microbiol. 72, 844–858. doi: 10.1111/j.1365-2958.2009.06699.x
Bak, E. N., Jensen, S. J. K., Nornberg, P., and Finster, K. (2016). Methylated silicates may explain the release of chlorinated methane from Martian soil. Earth Planet Sci Lett. 433, 226–231. doi: 10.1016/j.epsl.2015.10.044
Bak, E. N., Zafirov, K., Merrison, J. P., Jensen, S. J. K., Nørnberg, P., Gunnlaugsson, H. P., et al. (2017). Production of reactive oxygen species from abraded silicates. Implications for the reactivity of the martian soil. Earth Planet Sci Lett. 473C, 113–121. doi: 10.1016/j.epsl.2017.06.008
Banh, A., Chavez, V., Doi, J., Nguyen, A., Hernandez, S., Ha, V., et al. (2013). Manganese (Mn) oxidation increases intracellular Mn in Pseudomonas putida GB-1. PLoS ONE 8:e77835. doi: 10.1371/journal.pone.0077835
Bish, D. L., Blake, D. F., Vaniman, D. T., Chipera, S. J., Morris, R. V., Ming, D. W., et al. (2013). X-ray diffraction results from mars science laboratory: mineralogy of rocknest at gale crater. Science 341:1238932. doi: 10.1126/science.1238932
Buxton, G. V., Greenstock, C. L., Helman, W. P., and Ross, A. B. (1988). Critical-review of rate constants for reactions of hydrated electrons, hydrogen-atoms and hydroxyl radicals (.Oh/.O−) in aqueous-solution. J. Phys. Chem. Ref. Data. 17, 513–886. doi: 10.1063/1.555805
Cockell, C. S., Catling, D. C., Davis, W. L., Snook, K., Kepner, R. L., Lee, P., et al. (2000). The ultraviolet environment of mars: biological implications past, present, and future. Icarus 146, 343–359. doi: 10.1006/icar.2000.6393
Cortezzo, D. E., and Setlow, P. (2005). Analysis of factors that influence the sensitivity of spores of Bacillus subtilis to DNA damaging chemicals. J. Appl. Microbiol. 98, 606–617. doi: 10.1111/j.1365-2672.2004.02495.x
Daly, M. J., Gaidamakova, E. K., Matrosova, V. Y., Kiang, J. G., Fukumoto, R., Lee, D. Y., et al. (2010). Small-molecule antioxidant proteome-shields in deinococcus radiodurans. PLoS ONE 5:e12570 doi: 10.1371/journal.pone.0012570
Daly, M. J., Gaidamakova, E. K., Matrosova, V. Y., Vasilenko, A., Zhai, M., Venkateswaran, A., et al. (2004). Accumulation of Mn(II) in, Deinococcus radiodurans facilitates gamma-radiation resistance. Science 306, 1025–1028. doi: 10.1126/science.1103185
de La Vega, U. P., Rettberg, P., and Reitz, G. (2007). Simulation of the environmental climate conditions on martian surface and its effect on Deinococcus radiodurans. Adv. Space Res. 40, 1672–1677. doi: 10.1016/j.asr.2007.05.022
Diaz, B., and Schulze-Makuch, D. (2006). Microbial survival rates of Escherichia coli and Deinococcus radiodurans under low temperature, low pressure, and UV-Irradiation conditions, and their relevance to possible martian life. Astrobiology 6, 332–347. doi: 10.1089/ast.2006.6.332
Follonier, S., Panke, S., and Zinn, M. (2011). A reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from gluconate. Microb. Cell Fact. 10, 25–35. doi: 10.1186/1475-2859-10-25
Fubini, B., and Hubbard, A. (2003). Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis. Free Radic. Bio. Med. 34, 1507–1516. doi: 10.1016/S0891-5849(03)00149-7
Gunnlaugsson, H. P., Rasmussen, H., Madsen, M. B., and Nornberg, P. (2009). New analysis of the Mossbauer spectra of olivine basalt rocks from Gusev crater on Mars. Planet. Space Sci. 57, 640–645. doi: 10.1016/j.pss.2008.09.009
Hansen, A. A., Jensen, L. L., Kristoffersen, T., Mikkelsen, K., Merrison, J., Finster, K. W., et al. (2009). Effects of long-term simulated martian conditions on a freeze-dried and homogenized bacterial permafrost community. Astrobiology 9, 229–240. doi: 10.1089/ast.2008.0244
Hassler, D. M., Zeitlin, C., Wimmer-Schweingruber, R. F., Ehresmann, B., Rafkin, S., Eigenbrode, J. L., et al. (2014). Mars' surface radiation environment measured with the mars science laboratory's curiosity rover. Science 343:1244797. doi: 10.1126/science.1244797
Inaoka, T., Matsumura, Y., and Tsuchido, T. (1999). SodA and manganese are essential for resistance to oxidative stress in growing and sporulating cells of Bacillus subtilis. J. Bacteriol. 181, 1939–1943.
Jensen, S. J. K., Skibsted, J., Jakobsen, H. J., ten Kate, I. L., Guimlaugsson, H. P., Merrison, J. P., et al. (2014). A sink for methane on Mars? The answer is blowing in the wind. Icarus 236, 24–27. doi: 10.1016/j.icarus.2014.03.036
Johnson, A. P., Pratt, L. M., Vishnivetskaya, T., Pfiffner, S., Bryan, R. A., Dadachova, E., et al. (2011). Extended survival of several organisms and amino acids under simulated martian surface conditions. Icarus 211, 1162–1178. doi: 10.1016/j.icarus.2010.11.011
Kerney, K. R., and Schuerger, A. C. (2011). Survival of Bacillus subtilis endospores on ultraviolet-irradiated rover wheels and mars regolith under simulated martian conditions. Astrobiology 11, 477–485. doi: 10.1089/ast.2011.0615
Kiel, J. A. K. W., Boels, J. M., Beldman, G., and Venema, G. (1994). Glycogen in Bacillus-Subtilis- molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation. Mol. Microbiol. 11, 203–218. doi: 10.1111/j.1365-2958.1994.tb00301.x
Klotz, M. G., and Anderson, A. J. (1994). The role of catalase isozymes in the culturability of the root colonizer Pseudomonas-Putida after exposure to hydrogen-peroxide and antibiotics. Can. J. Microbiol. 40, 382–387. doi: 10.1139/m94-062
La Duc, M. T., Osman, S., Vaishampayan, P., Piceno, Y., Andersen, G., Spry, J. A., et al. (2009). Comprehensive census of bacteria in clean rooms by using DNA microarray and cloning methods. Appl. Environ. Microb. 75, 6559–6567. doi: 10.1128/AEM.01073-09
Mahaffy, P. R., Webster, C. R., Atreya, S. K., Franz, H., Wong, M., Conrad, P. G., et al. (2013). Abundance and isotopic composition of gases in the martian atmosphere from the curiosity rover. Science 341, 263–266. doi: 10.1126/science.1237966
Martin-Torres, F. J., Zorzano, M. P., Valentin-Serrano, P., Harri, A. M., Genzer, M., Kemppinen, O., et al. (2015). Transient liquid water and water activity at Gale crater on Mars. Nat. Geosci. 8, 357–361. doi: 10.1038/ngeo2412
Mason, J. M., and Setlow, P. (1986). Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light. J. Bacteriol. 167, 174–178. doi: 10.1128/jb.167.1.174-178.1986
Melly, E., Cowan, A. E., and Setlow, P. (2002). Studies on the mechanism of killing of Bacillus subtilis spores by hydrogen peroxide. J. Appl. Microbiol. 93, 316–325. doi: 10.1046/j.1365-2672.2002.01687.x
Mielecki, D., Saumaa, S., Wrzesinski, M., Maciejewska, A. M., Zuchniewicz, K., Sikora, A., et al. (2013). Pseudomonas putida AlkA and AlkB proteins comprise different defense systems for the repair of alkylation damage to DNA - In vivo, In vitro, and In silico Studies. PLoS ONE 8:e76198. doi: 10.1371/journal.pone.0076198
Moeller, R., Setlow, P., Horneck, G., Berger, T., Reitz, G., Rettberg, P., et al. (2008). Roles of the major, small, acid-soluble spore proteins and spore-specific and universal DNA repair mechanisms in resistance of Bacillus subtilis spores to ionizing radiation from x rays and high-energy charged-particle bombardment. J. Bacteriol. 190, 1134–1140. doi: 10.1128/JB.01644-07
Morris, R. V., Klingelhofer, G., Bernhardt, B., Schroder, C., Rodionov, D. S., de Souza, P. A., et al. (2004). Mineralogy at Gusev crater from the Mossbauer spectrometer on the Spirit rover. Science 305, 833–836. doi: 10.1126/science.1100020
Nagler, K., Setlow, P., Li, Y. Q., and Moeller, R. (2014). High salinity alters the germination behavior of bacillus subtilis spores with nutrient and nonnutrient germinants. Appl. Environ. Microb. 80, 1314–1321. doi: 10.1128/AEM.03293-13
Nicholson, W. L., and Setlow, P. (1990). “Sporulation, germination, and outgrowth,” in Molecular Biological Methods for Bacillus, eds C. R. Harwood and S. M. Cutting (Sussex: John Wiley and Sons), 391–450.
Ojha, L., Wilhelm, M. B., Murchie, S. L., McEwen, A. S., Wray, J. J., Hanley, J., et al. (2015). Spectral evidence for hydrated salts in recurring slope lineae on Mars. Nat. Geosci. 8, 829–832. doi: 10.1038/ngeo2546
Paulino-Lima, I. G., Janot-Pacheco, E., Galante, D., Cockell, C., Olsson-Francis, K., Brucato, J. R., et al. (2011). Survival of Deinococcus radiodurans against laboratory-simulated solar wind charged particles. Astrobiology 11, 875–882. doi: 10.1089/ast.2011.0649
Paulino-Lima, I. G., Pilling, S., Janot-Pacheco, E., de Brito, A. N., Barbosa, J., Leitao, A. C., et al. (2010). Laboratory simulation of interplanetary ultraviolet radiation (broad spectrum) and its effects on Deinococcus radiodurans. Planet. Space Sci. 58, 1180–1187. doi: 10.1016/j.pss.2010.04.010
Raguse, M., Fiebrandt, M., Denis, B., Stapelmann, K., Eichenberger, P., Driks, A., et al. (2016). Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization. J. Phys. D Appl. Phys. 49:285401. doi: 10.1088/0022-3727/49/28/285401
Riesenman, P. J., and Nicholson, W. L. (2000). Role of the spore coat layers in Bacillus subtilis spore resistance to hydrogen peroxide, artificial UV-C, UV-B, and solar UV radiation. Appl. Environ. Microb. 66, 620–626. doi: 10.1128/AEM.66.2.620-626.2000
Schuerger, A. C., Mancinelli, R. L., Kern, R. G., Rothschild, L. J., and McKay, C. P. (2003). Survival of endospores of Bacillus subtilis on spacecraft surfaces under simulated martian environments: implications for the forward contamination of Mars. Icarus 165, 253–276. doi: 10.1016/S0019-1035(03)00200-8
Setlow, B., and Setlow, P. (1993). Binding of small, acid-soluble spore proteins to DNA plays a significant role in the resistance of Bacillus subtilis spores to hydrogen peroxide. Appl. Environ. Microbiol. 59, 3418–3423.
Setlow, B., Atluri, S., Kitchel, R., Koziol-Dube, K., and Setlow, P. (2006). Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins. J. Bacteriol. 188, 3740–3747. doi: 10.1128/JB.00212-06
Setlow, P. (2014). “Spore resistance properties,” in The Bacterial Spore: From Molecules to Systems, eds P. Eichenberger and A. Driks (Washington, DC: American Sociaty for Microbiology), 201–215. doi: 10.1128/microbiolspec.TBS-0003-2012
Slieman, T. A., and Nicholson, W. L. (2001). Role of dipicolinic acid in survival of Bacillus subtilis spores exposed to artificial and solar UV radiation. Appl. Environ. Microb. 67, 1274–1279. doi: 10.1128/AEM.67.3.1274-1279.2001
Smith, S. A., Benardini, J. N., Anderl, D., Ford, M., Wear, E., Schrader, M., et al. (2017). Identification and characterization of early mission phase microorganisms residing on the mars science laboratory and assessment of their potential to survive mars-like conditions. Astrobiology 17, 253–265. doi: 10.1089/ast.2015.1417
Svenningsen, N. B., Perez-Pantoja, D., Nikel, P. I., Nicolaisen, M. H., de Lorenzo, V., and Nybroe, O. (2015). Pseudomonas putida mt-2 tolerates reactive oxygen species generated during matric stress by inducing a major oxidative defense response. BMC Microbiol. 15:202. doi: 10.1186/s12866-015-0542-1
Tian, B., Xu, Z. J., Sun, Z. T., Lin, J., and Hua, Y. J. (2007). Evaluation of the antioxidant effects of carotenoids from Deinococcus radiodurans through targeted mutagenesis, chemiluminescence, and DNA damage analyses. Biochim. Et Biophys. Acta Gen. Subjects 1770, 902–911. doi: 10.1016/j.bbagen.2007.01.016
Tuleta, M., Gabla, L., and Szkarlat, A. (2005). Low-energy ion bombardment of frozen bacterial spores and its relevance to interplanetary space. Europhys. Lett. 70, 123–128. doi: 10.1209/epl/i2004-10471-3
Keywords: habitability, erosion, reactive oxygen species, forward contamination, stress factors, saltation, toxicity, microorganisms
Citation: Bak EN, Larsen MG, Moeller R, Nissen SB, Jensen LR, Nørnberg P, Jensen SJK and Finster K (2017) Silicates Eroded under Simulated Martian Conditions Effectively Kill Bacteria—A Challenge for Life on Mars. Front. Microbiol. 8:1709. doi: 10.3389/fmicb.2017.01709
Received: 30 June 2017; Accepted: 23 August 2017;
Published: 12 September 2017.
Edited by:Daniela Billi, Università degli Studi di Roma Tor Vergata, Italy
Reviewed by:Aude Picard, Harvard University, United States
Timothy Ferdelman, Max Planck Institute for Marine Microbiology, Germany
Copyright © 2017 Bak, Larsen, Moeller, Nissen, Jensen, Nørnberg, Jensen and Finster. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Ebbe N. Bak, email@example.com