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Front. Microbiol. | doi: 10.3389/fmicb.2018.00328

Characterization of resistance patterns and detection of apramycin resistance genes in Escherichia coli isolated from chicken feces and houseflies after apramycin administration

 Anyun Zhang1, Zhongbin Guan1,  Hongmei Tuo1, Dan Liu1, Yanxian Yang1,  Yunxia Li1,  Changwen Xu1, Changwei Lei1 and  Hong-Ning Wang1*
  • 1Sichuan University, China

The aim of this study was to evaluate the influence of apramycin administration on the development of antibiotic resistance in Escherichia coli (E. coli) strains isolated from chicken feces and houseflies under field conditions. Chickens in the medicated group (n=25000) were given successive prophylactic doses (0.5 mg/L) of apramycin in their drinking water from Days 1 through 5, while no antibiotics were added to the un-medicated groups drinking water (n=25000). Over 40 days, a total of 1170 E. coli strains were isolated from fecal samples obtained from medicated and un-medicated chickens and houseflies from the same chicken farm. Apramycin MIC90 values for E. coli strains obtained from the medicated group increased 32-128 times from Days 2 to 6 (256μg/ml to 1024 μg/ml) when compared to those on Day 0 (8 μg/ml). Strains isolated from un-medicated chickens and houseflies had consistently low MIC90 values (8 μg/ml to 16 μg/ml) during the first week, but showed a dramatic increase from Days 8 to 10 (128μg/ml to 1024 μg/ml). The apramycin resistance gene aac(3)-Ⅳ was detected in E. coli strains from medicated (n=71), un-medicated (n=32), and housefly groups (n=42). All strains positive for aac(3)-Ⅳ were classified into 12 pulsed-field gel electrophoresis (PFGE) types. PFGE type A, E, and G were the predominant types in both the medicated and housefly groups, suggesting houseflies play an important role in spreading E. coli resistant strains. Taken together, our study revealed that apramycin administration could facilitate the occurrence of apramycin-resistant E.coli and the apramycin-resistance gene acc(3)-IV. In turn, these strains could be transmitted by houseflies, thus increasing the potential risk of spreading multi-drug resistant E. coli to the public.

Keywords: apramycin resistance genes, Escherichia coli, PFGE, Chicken feces, Housefly

Received: 11 Aug 2017; Accepted: 12 Feb 2018.

Edited by:

Gilberto Igrejas, University of Trás-os-Montes and Alto Douro, Portugal

Reviewed by:

Liang Li, LA BioMed, United States
Sunil D. Saroj, Symbiosis International University, India  

Copyright: © 2018 Zhang, Guan, Tuo, Liu, Yang, Li, Xu, Lei and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Hong-Ning Wang, Sichuan University, Chengdu, China, whongning@163.com