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Front. Microbiol. | doi: 10.3389/fmicb.2018.00330

Identification of a novel serum biomarker for tuberculosis infection in Chinese HIV patients by iTRAQ-based quantitative proteomics

 Cong Chen1, Tao Yan2, Liguo Liu2,  Jianmin Wang1 and Qi Jin1*
  • 1Institute of Pathogen Biology (CAMS), China
  • 2MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, China

Tuberculosis (TB) is a major comorbidity in HIV patients as well as a serious co-epidemic. Traditional detection methods are not effective or sensitive for the detection of Mycobacterium tuberculosis at the early stage. TB has become a major cause of lethal on HIV patients. In the study, we employed isobaric tags for relative and absolute quantitation (iTRAQ) technology to identify the different host responses in HIV-noTB and HIV-TB patients’ sera. Given the diversity of HIV subtypes, which results in a variety of host responses in different human populations, we focused on Chinese patients. Of the 25 proteins identified, 7 were increased and 18 were decreased in HIV-TB co-infected patients. These proteins were found to be involved in similar immune response processes. Importantly, we identified a candidate protein, Endoglin (ENG), which showed a 4.9 times increase by iTRAQ method and 11.5 times increase by ELISA. ENG demonstrated the diagnostic efficacy and presented a novel molecular biomarker for TB in HIV-infected Chinese patients. This study provides new insight into the challenges in the diagnosis and effective management of patients with HIV-TB.

Keywords: HIV, Tuberculosis, biomarkers, Proteomics., iTRAQ-based proteomics

Received: 03 Nov 2017; Accepted: 12 Feb 2018.

Edited by:

Baolei Jia, Chung-Ang University, South Korea

Reviewed by:

Kuldeep Dhama, Indian Veterinary Research Institute, India
Ranran Wang, Perelman School of Medicine, University of Pennsylvania, United States
Chao Qiu, Fudan University, China  

Copyright: © 2018 Chen, Yan, Liu, Wang and Jin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Qi Jin, Institute of Pathogen Biology (CAMS), Beijing, China, zdsys@vip.sina.com