A Retrospective Review of Microbiological Methods Applied in Studies Following the Deepwater Horizon Oil Spill
- 1Shantou University, China
The Deepwater Horizon (DWH) oil spill in the Gulf of Mexico in 2010 resulted in serious damage to local marine and coastal environments. In addition to the physical removal and chemical dispersion of spilled oil, biodegradation by indigenous microorganisms was regarded as the most effective way for cleaning up residual oil. Different microbiological methods were applied to investigate the changes and responses of bacterial communities after the DWH oil spills. By summarizing and analyzing these microbiological methods, giving recommendations and proposing some methods that have not been used, this review aims to provide constructive guidelines for microbiological studies after environmental disasters, especially those involving organic pollutants.
Keywords: Pure culture, clone library, Denaturing Gradient Gel Electrophoresis, pyrosequencing, META-OMICS, stable isotope probing, Molecular ecological networks
Received: 23 Aug 2017;
Accepted: 08 Mar 2018.
Edited by:Michail M. Yakimov, Consiglio Nazionale Delle Ricerche (CNR), Italy
Reviewed by:Martin Krüger, Federal Institute For Geosciences and Natural Resources, Germany
Tony Gutierrez, Heriot-Watt University, United Kingdom
Copyright: © 2018 Zhang, Hu and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Hui Wang, Shantou University, Shantou, China, email@example.com