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Front. Microbiol. | doi: 10.3389/fmicb.2018.01114

CcpA-dependent Carbon Catabolite Repression Regulates Fructooligosaccharides Metabolism in Lactobacillus plantarum

 Chen Chen1,  Yanqing Lu1,  Linlin Wang1,  Haiyan Yu1 and Huaixiang Tian1*
  • 1Food Science and Technology, Shanghai Institute of Technology, China

Fructooligosaccharides (FOS) metabolism in Lactobacillus plantarum is controlled by two gene clusters, and the global regulator catabolite control protein A (CcpA) may be involved in the regulation. To understand the mechanism, this study focused on the regulation relationships of CcpA towards target genes and the binding effects on the catabolite responsive element (cre). First, reverse transcription-PCR analysis of the transcriptional organization of the FOS-related gene clusters showed that they were organized in three independent polycistronic units. Diauxic growth, hierarchical utilization of carbohydrates and repression of FOS-related genes were observed in cultures containing FOS and glucose, suggesting carbon catabolite repression (CCR) control in FOS utilization. Knockout of ccpA gene eliminated these phenomena, indicating the principal role of this gene in CCR of FOS metabolism. Furthermore, six potential cre sites for CcpA binding were predicted in the regions of putative promoters of the two clusters. Direct binding was confirmed by electrophoretic mobility shift assays (EMSAs) in vitro and chromatin immunoprecipitation (ChIP) in vivo. The results of the above studies suggest that CcpA is a vital regulator of FOS metabolism in L. plantarum and that CcpA-dependent CCR regulates FOS metabolism through the direct binding of CcpA towards the cre sites in the promoter regions of FOS-related clusters.

Keywords: Lactobacillus plantarum, Fructooligosaccharide, Catabolite control protein A, Carbon catabolite repression, Metabolic Regulation

Received: 17 Mar 2018; Accepted: 11 May 2018.

Edited by:

Zhihong Sun, Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education, Inner Mongolia Agricultural University, China

Reviewed by:

Kuan Wang, Tianjin University of Science and Technology, China
Lianzhong Ai, University of Shanghai for Science and Technology, China  

Copyright: © 2018 Chen, Lu, Wang, Yu and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Huaixiang Tian, Shanghai Institute of Technology, Food Science and Technology, 100 Haiquan Road, 201418, Shanghai, China, tianhx@sit.edu.cn