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Front. Microbiol. | doi: 10.3389/fmicb.2018.01375

Analysis on metabolic functions of stored rice microbial communities by BIOLOG ECO microplates

 Zhiwen Ge1,  Hengjun Du1, Yulong Gao1 and  Weifen Qiu1*
  • 1Nanjing University of Finance and Economics, China

Microbial contamination has been a pervasive issue during the rice storage and triggers extensive researches. The metabolism of microorganisms was proved as an indicator to mirror the degree of microbial contamination. It is necessary to develop a scientific method to analyze the metabolism of rice microbial communities, thereby monitoring the microbial contamination. In this study, the metabolism of rice microbial communities in different storing-year were investigated by BIOLOG ECO microplates. The three rice samples were respectively stored for one, two, and three years. The related indicators of BIOLOG ECO microplates were determined, including average well color development (AWCD) of carbon sources and three metabolic functional diversity indices. The results showed that there were significant differences in the AWCD of all carbon sources among the three rice microbial communities (p < 0.05), and the functional diversity indices except Simpson index showed significant differences (p < 0.05). Additionally, the three rice microbial communities differed significantly in the metabolic utilization of carboxylic acids and miscellaneous (p < 0.05), and there were, however, no significant differences in the other four types of carbon sources. Furthermore, principal component analysis revealed that the microbial communities of stored rice had obviously different metabolic functions in different storage period. Therefore, the study indicated that the BIOLOG ECO microplate was applicable to evaluate the metabolic functions of rice microbial communities, and carboxylic acids and miscellaneous were two crucial parameters of carbon sources to identify the metabolic differences of microbial communities, a case in which it reflected the conditions of rice microbial contamination.

Keywords: rice, microbial communities, BIOLOG Eco MicroPlates, AWCD, Principal Component Analysis, metabolic functions

Received: 06 Jan 2018; Accepted: 06 Jun 2018.

Edited by:

Chunbao Li, Nanjing Agricultural University, China

Reviewed by:

Fusheng Chen, Henan University of Technology, China
Na Na Wu, Academy of State Administration of Grain, China  

Copyright: © 2018 Ge, Du, Gao and Qiu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Weifen Qiu, Nanjing University of Finance and Economics, Nanjing, 210023, Jiangsu, China, weifenqiu711@163.com