Impact Factor 4.019

The world's most-cited Microbiology journal

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2018.01543

Effects of nitrogen and phosphorus inputs on soil bacterial abundance, diversity and community composition in Chinese fir plantations

 Qing Wang1*, Cong Wang2, Weiwei Yu3, Ali Turak4*, Diwen Chen1, Ying Huang1, Yong Jiang1, Zhengrui Huang1 and Junhua Ao1*
  • 1Guangzhou Sugarcane Industry Research Institute, China
  • 2Guangdong Institute of Eco-environmental and Soil Sciences (CAS), China
  • 3Research Center for Eco-environmental Sciences (CAS), China
  • 4Science and Technology Management Dept. Sinofert, China

Abstract
Nutrient inputs to forest ecosystems significantly influence aboveground plant community structure and ecosystem functioning. However, our knowledge of the influence of nitrogen (N) and/or phosphorus (P) inputs on belowground microbial communities in subtropical forests is still unclear. In this study, we used quantitative PCR and Illumina Miseq sequencing of the bacterial 16S rRNA gene to investigate bacterial abundance, diversity and community composition in a Chinese fir plantation. The fertilization treatments were: no fertilizer (CK), P amendment (P), N amendment (N) and N with P amendment (NP). Additions of N decreased soil pH and bacterial 16S rRNA gene abundance by 3.95 (from 4.69 to 3.95) and 3.95×109 copies g-1 dry soil (from 9.27×109 to 3.95×109 g-1 dry soil), respectively. Bacterial richness and diversity decreased with N addition (N and NP) rather than only P input. Proteobacteria, Acidobacteria, and Actinobacteria were the predominant phyla in all treatments. Nitrogen addition increased the relative abundance of Proteobacteria and Actinobacteria by 42.0% and 10.5%, respectively, while it reduced that of Acidobacteria by 26.5%. Bacterial communities in the CK and P treatment separated from those in the N and NP treatments upon Principle Coordinates Analysis (PCoA). Phosphorus addition did not significantly affect soil bacterial communities, and no interactions between N and P inputs on microbial traits were observed. Soil pH and mineral N availability (NH4+-N and NO3--N) appeared to have a cooperative effect on bacterial abundance and community structure, with soil pH being the key influencing factor by Canonical Correspondence Analysis (CCA). These results indicate that inorganic N rather than P fertilization affected both bacterial abundance and community composition in subtropical forests.
Keywords: Soil bacterial community, N and P inputs, Illumina Miseq sequencing, Chinese fir plantation, 16S rRNA gene

Keywords: Soil bacterial community, N and P inputs, Illumina Miseq sequencing, Chinese Fir Plantation, 16S rRNA gene

Received: 18 Feb 2018; Accepted: 20 Jun 2018.

Edited by:

Marcus A. Horn, Leibniz Universität Hannover, Germany

Reviewed by:

Tida Ge, Institute of Subtropical Agriculture (CAS), China
Katharina Kujala, University of Oulu, Finland  

Copyright: © 2018 Wang, Wang, Yu, Turak, Chen, Huang, Jiang, Huang and Ao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
PhD. Qing Wang, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China, wangqing316000@163.com
PhD. Ali Turak, Science and Technology Management Dept. Sinofert, Beijing, China, 308850239@qq.com
PhD. Junhua Ao, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China, huangqiong870730@163.com