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Front. Microbiol. | doi: 10.3389/fmicb.2018.02211

A Model for Trans-Kingdom Pathogenicity in Fonsecaea Agents of Human Chromoblastomycosis

 Gheniffer Fornari1, 2,  Renata R. Gomes1, 2, Juliana Degenhardt-Goldbach3,  Bruna Jacomel de Souza Lima1, 2,  Morgana Ferreira Voidaleski1, 2,  Suelen S. Dos Santos4,  Sandro R. Almeida4, Marisol Dominguez Muro5, 6, Cleusa Bonna2, 7,  Rosana H. Scola6,  Edvaldo Trindade2, 8,  Israel H. Bini2, 8,  Lisandra Maba2, 8, Daiane kestring3,  Mariana M. Nascimento1, 2, Douglas A. Steinmacher9,  Bruna Soley2, Shuwen Deng10,  Moises B. Da Silva11,  Claudio G. Salgado11, Anamelia Lorenzetti Bocca12,  Conceição M. Pedroso e Silva de Azevedo13,  Vania Aparecida Vicente A. Vicente1, 2* and  Sybren De Hoog1, 2, 10, 14, 15*
  • 1Department of Basic Pathology, Universidade Federal do Paraná, Brazil
  • 2Department of Pharmacology, Universidade Federal do Paraná, Brazil
  • 3Embrapa Florestas, Brazil
  • 4Department of Clinical and Pharmacological Analysis, College of Pharmaceutical Sciences, Universidade de São Paulo, Brazil
  • 5Support and Diagnosis Unit, Hospital de Clínicas, Universidade Federal do Paraná, Brazil
  • 6Hospital de Clínicas, Universidade Federal do Paraná, Brazil
  • 7Department of Botany, Universidade Federal do Paraná, Brazil
  • 8Department of Cellular Biology, Universidade Federal do Paraná, Brazil
  • 9Vivetech Agrociências, Brazil
  • 10Department of Medical Microbiology, Suzhou High-tech Zone People's Hospital, China
  • 11Dermato-Immunology Laboratory, Institute of Biological Sciences (ICB) of Federal University of Pará, Brazil
  • 12Department of Cell Biology, Universidade de Brasília, Brazil
  • 13Department of Medicine, Universidade Federal do Maranhão, Brazil
  • 14Westerdijk Fungal Biodiversity Institute, Netherlands
  • 15Centre of Expertise in Mycology, Radboud University Medical Center, Netherlands

The fungal genus Fonsecaea comprises etiological agents of human chromoblastomycosis, a chronic implantation skin disease. The current hypothesis is that patients acquire the infection through an injury from plant material. The present study aimed to evaluate a model of infection in plant and animal hosts to understand the parameters of trans-Kingdom pathogenicity. Clinical strains of causative agents of chromoblastomycosis were compared with a strain from a living plant. The clinical strains of F. monophora and F. pedrosoi were concentrated near the epidermis, whereas F. erecta colonized deeper plant tissues, resembling an endophytic behavior. In an invertebrate infection model with larvae of a beetle, Tenebrio molitor, F. erecta exhibited the lowest survival rates. However, F. pedrosoi produced dark, spherical to ovoidal cells that resembled muriform cells, the invasive form of human chromoblastomycosis confirming the role of muriform cells as a pathogenic adaptation in animal tissues. An immunologic assay in BALB/c mice demonstrated the high virulence of saprobic species in animal models was subsequently controlled via host higher immune response.

Keywords: Fonsecaea species, Chromoblastomycosis, Virulence tests, Mimosa pudica, Bactris gasipaes, Tenebrio molitor, animal model, Plant model

Received: 01 Apr 2018; Accepted: 29 Aug 2018.

Edited by:

Orazio Romeo, Università degli Studi di Messina, Italy

Reviewed by:

Gil Benard, Universidade de São Paulo, Brazil
Peiying Feng, Sun Yat-sen University, China  

Copyright: © 2018 Fornari, Gomes, Degenhardt-Goldbach, Jacomel de Souza Lima, Ferreira Voidaleski, Dos Santos, Almeida, Dominguez Muro, Bonna, Scola, Trindade, Bini, Maba, kestring, Nascimento, Steinmacher, Soley, Deng, Da Silva, Salgado, Lorenzetti Bocca, Pedroso e Silva de Azevedo, Vicente and De Hoog. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Vania Aparecida Vicente A. Vicente, VV., Universidade Federal do Paraná, Department of Basic Pathology, Curitiba, Brazil, vaniava63@gmail.com
Dr. Sybren De Hoog, SDH., Universidade Federal do Paraná, Department of Basic Pathology, Curitiba, Brazil, s.hoog@westerdijkinstitute.nl