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Front. Microbiol. | doi: 10.3389/fmicb.2018.02252

Novel antifungal compounds, spermine-like and short cyclic polylactates, produced by Lactobacillus harbinensisK.V9.3.1Np in yogurt

  • 1Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Tunisia
  • 2Univ Rennes, CNRS, ISCR–UMR 6226, F-35000 Rennes, France., University of Rennes 1, France
  • 3UEB, EA3882, SFR148 ScInBioS, ESIAB, Technopôle de Brest-Iroise, Plouzané, France., Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Bretagne Occidentale, France
  • 4CRMPO, University of Rennes 1, France
  • 5UMR6197 Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), France

Lactobacillus harbinensis K.V9.3.1Np was described as endowed with high antifungal activity. Most of the studies associated this activity to the produced organic acids, i.e. lactic acid, acetic acid and hexanoic acid. The aim of this study was to purify and identify, other not yet described, antifungal molecules produced by L. harbinensis K.V9.3.1Np. when used in yogurt fermentation. Active compounds were extracted through several extraction processes using organic solvents and protein precipitation. The fractions of interest were purified using flash chromatography and preparative HPLC for specific characterization. The bioactive compounds identification was performed using Nuclear Magnetic Resonance and Mass Spectrometry. Activity tests against Penicillium expansum and Yarrowia lipolytica showed that the active compounds from L. harbinensis K.V9.3.1Np. are the benzoic acid and a polyamine identified as a spermine analogue, which has not been reported earlier. However, the highest activity was shown by a mixture of short (n=2 to n=5) polycyclic lactates. Our overall results demonstrate the efficiency of the proposed extraction/purification approach. The new compounds described here have promising antifungal activities but further studies are still needed to decipher their mode of action and production pathways. Even though, they present an interesting potential application in food, feed, as well as, in pharmaceutical industries and could serve as alternative to chemical additives.

Keywords: polylactates, Lactobacillus, extraction, purification, MS, NMR, Spermine

Received: 23 Apr 2018; Accepted: 04 Sep 2018.

Edited by:

Rosalba Lanciotti, Università degli Studi di Bologna, Italy

Reviewed by:

Giuseppe Spano, University of Foggia, Italy
Nuno P. Mira, Instituto de Bioengenharia e Biociências (IBB), Portugal  

Copyright: © 2018 Mosbah, delavenne, Souissi, jehan, le yondre, Cherif, bondon, Mounier, baudy floch and le blay. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Amor Mosbah, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Sidi Thabet, Tunisia, amor.mosbah@gmail.com