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Front. Microbiol. | doi: 10.3389/fmicb.2018.02717

Genetic differentiation, diversity and drug susceptibility of Candida krusei

jie Gong1,  Meng Xiao2, 3,  He Wang2, 3,  Timothy Kudinha4, 5, Yu Wang6,  Weiwei Wu7, Lihua He1,  Yingchun Xu2, 3* and  Jianzhong Zhang1*
  • 1State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, China
  • 2Peking Union Medical College Hospital (CAMS), China
  • 3Beijing Key Laboratory of Invasive Fungal Disease Research and Accurate Diagnosis, China
  • 4Charles Sturt University, Australia
  • 5Central West Pathology Laboratory, Australia
  • 6Zhejiang Key Laboratory of Wildlife Biotechnology and Protection and Utilization, Zhejiang Normal University, China
  • 7Department of Dermatology, Hainan Provincial Center for Skin Disease and STI Control, China

Candida krusei is a notable pathogenic fungus that causes invasive candidiasis, mainly due to its natural resistance to fluconazole. However, to date, there is limited research on the genetic population features of C. krusei. We developed a set of microsatellite markers for this organism, with a cumulative discriminatory power of 1.000. Using these microsatellite loci, 48 independent C. krusei strains of clearly known the sources, were analyzed. Furthermore, susceptibility to 9 antifungal agents was determined for each strain, by the Clinical and Laboratory Standards Institute broth microdilution method. Population structure analyses revealed that C. krusei could be separated into two clusters. The cluster with the higher genetic diversity had wider MIC ranges for six antifungal agents. Furthermore, the highest MIC values of the six antifungal agents belonged to the cluster with higher genetic diversity. The higher genetic diversity cluster might have a better adaptive capacity when C. krusei is under selection pressure from antifungal agents, and thus is more likely to develop drug resistance.

Keywords: Candida krusei, Invasive candidiasis, genetic differentiation, genetic diversity, microsatellites, Drug Susceptibility

Received: 19 Jul 2018; Accepted: 24 Oct 2018.

Edited by:

Silvia Buroni, University of Pavia, Italy

Reviewed by:

Avi Peretz, The Baruch Padeh Medical Center, Poriya, Israel
Paula Sampaio, University of Minho, Portugal
Sona Kucharikova, University of Trnava, Slovakia  

Copyright: © 2018 Gong, Xiao, Wang, Kudinha, Wang, Wu, He, Xu and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Yingchun Xu, Peking Union Medical College Hospital (CAMS), Beijing, China, xycpumch@139.com
Prof. Jianzhong Zhang, State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, 102206, Beijing Municipality, China, zhangjianzhong@icdc.cn