Original Research ARTICLE
Enhancement of pneumocandin B0 production in Glarea lozoyensis by low-temperature adaptive laboratory evolution
- 1Nanjing Tech University, China
- 2Nanjing University of Chinese Medicine, China
- 3Tianjin University, China
The production of pneumocandin B0 is limited by feedback inhibition. Here, low-temperature adaptive laboratory evolution (ALE) was used to improve the production capacity of Glarea lozoyensis by enhancing its membrane permeability. After 50 cycles of ALE, the pneumocandin B0 production of the endpoint strain (ALE50) reached 2131 g/L, which was 32% higher than that of the starting strain (ALE0). ALE50 showed a changed fatty acid composition of the cell membrane, which increased its permeability by 14%, which in turn increased the secretion ratio 3-fold. Furthermore, ALE50 showed increased intracellular proline and acetyl-CoA concentrations, superoxide dismutase and, catalase activity, as well as total antioxidant capacity. The slight biomass decrease in ALE50 was accompanied by enhanced isocitrate dehydrogenase and reduced ATP:citrate lyase activity. Finally, a putative model of the accumulation and secretion of pneumocandin B0 in ALE50 was established. ALE is a promising method to release intracellular feedback inhibition.
Keywords: Fatty acid synthesis regulation, Membrane Permeability, adaptive evolution, enzyme activity, Glarea lozoyensis, pneumocandin B0
Received: 22 Aug 2018;
Accepted: 30 Oct 2018.
Edited by:Marie-Joelle VIROLLE, Centre national de la recherche scientifique (CNRS), France
Reviewed by:I-Son Ng, National Cheng Kung University, Taiwan
Chang Dou, University of Washington, United States
Copyright: © 2018 Song, Zhang, Zhang, Huang, Ji, Ren, Wen and Huang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Sen Zhang, Nanjing University of Chinese Medicine, Nanjing, China, email@example.com