Corrigendum: A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping
- 1Wellness Promotion Science Center, Kanazawa University, Japan
A Corrigendum on: A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping
by Ogai, K., Nagase, S., Mukai, K., Iuchi, T., Mori, Y., Matsue, M., Sugitani, K., Sugama, J. and Okamoto, S. (2018). Front Microbiol. 9:2362. doi: 10.3389/fmicb.2018.02362
In the original article, there was an error in the sequence of the forward primer used in the real-time PCR.
The forward primer 5′-ACTGAGACACGGYCCA-3′ in the original text should read 5′-ACTGAGAYACGGYCCA-3′. The primer with the corrected sequence was actually used in the study; therefore, the results are not affected.
A correction has been made to Materials and Methods, Real-Time PCR:
To determine the copy number of the 16S rRNA gene in the DNA extracted from the swab or adhesive tape, real-time PCR was performed. The 16S rRNA gene was amplified using universal primer pairs (F: 5′-ACTGAGAYACGGYCCA-3′; R: 5′-CTGCTGGCACGDAGTTAGCC-3′) (Wang and Qian, 2009) and a universal probe (5′-VIC-ACTGCTGCCTCCCGTA-NFQ-MGB-3′) (Gao et al., 2010) with the Thunderbird® Probe qPCR Mix (Toyobo Co., Ltd., Osaka, Japan). A standard curve was drawn from a known amount of the 16S rRNA gene [100, 10, 1, and 0.1 pg of Propionibacterium acnes genomes, which are equivalent to 7.23 × 104, 7.23 × 103, 7.23 × 102, and 7.23 × 101 16S rRNA genes, respectively (Nadkarni et al., 2002; Miura et al., 2010; Stoddard et al., 2015)]. All the reactions were performed with the Mx3005P System (Agilent Technologies, CA, United States). The copy number of 16S rRNA gene was compared for the same size of skin area (4.4 × 4.4-cm square; Supplementary Figure 1, open squares).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Keywords: Skin microbiome, Swabbing, tape stripping, Bacterial culture, next generation seqencing
Received: 31 Oct 2018;
Accepted: 01 Nov 2018.
Edited by:Frontiers I. Microbiology Editorial Office, Frontiers Media SA, Switzerland
Copyright: © 2018 Ogai, Nagase, Mukai, Iuchi, Mori, Matsue, Sugitani, Sugama and Okamoto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Shigefumi Okamoto, Kanazawa University, Wellness Promotion Science Center, Kanazawa, Japan, email@example.com