Corrigendum: A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping
- 1Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan
- 2Department of Clinical Laboratory Science, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan
- 3Department of Clinical Nursing, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan
- 4Advanced Health Care Science Research Unit, Innovative Integrated Bio-Research Core, Institute for Frontier Science Initiative, Kanazawa University, Kanazawa, Japan
by Ogai, K., Nagase, S., Mukai, K., Iuchi, T., Mori, Y., Matsue, M., et al. (2018). Front. Microbiol. 9:2362. doi: 10.3389/fmicb.2018.02362
In the original article, there was an error in the sequence of the forward primer used in the real-time PCR.
The forward primer 5′-ACTGAGACACGGYCCA-3′ in the original text should read 5′-ACTGAGAYACGGYCCA-3′. The primer with the corrected sequence was actually used in the study; therefore, the results are not affected.
A correction has been made to Materials and Methods, Real-Time PCR:
To determine the copy number of the 16S rRNA gene in the DNA extracted from the swab or adhesive tape, real-time PCR was performed. The 16S rRNA gene was amplified using universal primer pairs (F: 5′-ACTGAGAYACGGYCCA-3′; R: 5′-CTGCTGGCACGDAGTTAGCC-3′) (Wang and Qian, 2009) and a universal probe (5′-VIC-ACTGCTGCCTCCCGTA-NFQ-MGB-3′) (Gao et al., 2010) with the Thunderbird® Probe qPCR Mix (Toyobo Co., Ltd., Osaka, Japan). A standard curve was drawn from a known amount of the 16S rRNA gene [100, 10, 1, and 0.1 pg of Propionibacterium acnes genomes, which are equivalent to 7.23 × 104, 7.23 × 103, 7.23 × 102, and 7.23 × 101 16S rRNA genes, respectively (Nadkarni et al., 2002; Miura et al., 2010; Stoddard et al., 2015)]. All the reactions were performed with the Mx3005P System (Agilent Technologies, CA, United States). The copy number of 16S rRNA gene was compared for the same size of skin area (4.4 × 4.4-cm square; Supplementary Figure 1, open squares).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Miura, Y., Ishige, I., Soejima, N., Suzuki, Y., Uchida, K., Kawana, S., et al. (2010). Quantitative PCR of Propionibacterium acnes DNA in samples aspirated from sebaceous follicles on the normal skin of subjects with or without acne. J. Med. Dent. Sci. 57, 65–74. doi: 10.11480/jmds.570108
Nadkarni, M. A., Martin, F. E., Jacques, N. A., and Hunter, N. (2002). Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148(Pt 1), 257–266. doi: 10.1099/00221287-148-1-257
Stoddard, S. F., Smith, B. J., Hein, R., Roller, B. R., and Schmidt, T. M. (2015). rrnDB: improved tools for interpreting rRNA gene abundance in bacteria and archaea and a new foundation for future development. Nucleic Acids Res. 43, D593–D598. doi: 10.1093/nar/gku1201
Keywords: skin microbiome, swabbing, tape stripping, bacterial culture, next generation sequencing
Citation: Ogai K, Nagase S, Mukai K, Iuchi T, Mori Y, Matsue M, Sugitani K, Sugama J and Okamoto S (2018) Corrigendum: A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping. Front. Microbiol. 9:2812. doi: 10.3389/fmicb.2018.02812
Received: 31 October 2018; Accepted: 01 November 2018;
Published: 20 November 2018.
Approved by:Frontiers in Microbiology Editorial Office, Frontiers Media SA, Switzerland
Copyright © 2018 Ogai, Nagase, Mukai, Iuchi, Mori, Matsue, Sugitani, Sugama and Okamoto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Shigefumi Okamoto, email@example.com
†These authors have contributed equally to this work
‡Present Address: Terumi Iuchi, Department of Nursing, Faculty of Health Sciences, Komatsu University, Komatsu, Japan