Original Research ARTICLE
Morin protects channel catfish from Aeromonas hydrophila infection by blocking aerolysin activity
- 1Yangtze River Fisheries Research Institute, China
Aeromonas hydrophila (A. hydrophila) is an opportunistic bacterial pathogen widely distributed in the environments, particular aquatic environment. The pathogen can cause a range of infections both in human and animals including fishes. However, the application of antibiotics in treatment of A. hydrophila infections leads to the emergence of resistant strains. Consequently, new approaches need to be developed in fighting this pathogen. Aerolysin, the chief virulence factor produced by pathogenic A. hydrophila strains has been employed as target identifying new drugs. In our present study, we found that morin, a flavonoid without anti-bacterial activity isolated from traditional Chinese medicine, could directly inhibit the hemolytic activity of aerolysin.
To determine the binding sites and the action of mechanism of morin against AerA, several assays were performed. Ser36, Pro347 and Arg356 were identified as the main binding sites affecting the conformation of AerA and resulted in block of the heptameric formation. Moreover, morin could protect Vero cells from cell injury mediated by aerolysin. In vivo study showed that morin could provide a protection to channel catfish against A. hydrophila infection. These results demonstrated that morin could be developed as a promising candidate for the treatment of A. hydrophila infections by decreasing the pathogenesis of A. hydrophila.
Keywords: Aeromonas hydrophila, Aerolysin, Morin, anti-virulence, antibiotics
Received: 11 Sep 2018;
Accepted: 05 Nov 2018.
Edited by:Natalia V. Kirienko, Rice University, United States
Reviewed by:Durg V. Singh, Institute of Life Sciences (ILS), India
Iddya Karunasagar, Nitte University, India
Copyright: © 2018 Ai, dong, liu, xu and yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Xiaohui Ai, Yangtze River Fisheries Research Institute, Wuhan, China, email@example.com