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Front. Microbiol. | doi: 10.3389/fmicb.2018.02853

Complete genome sequence of blaIMP-6-positive Metakosakonia sp. MRY16-398 isolate from the ascites of a diverticulitis patient

 Tsuyoshi Sekizuka1, Mari Matsui1, Tomiyo Takahashi2, Michiko Hayashi1,  Satowa Suzuki1, Akihiko Tokaji2 and  Makoto Kuroda1*
  • 1National Institute of Infectious Diseases (NIID), Japan
  • 2Department of Health Policy, Kochi Prefecture, Japan

A novel species of carbapenemase-producing Enterobacteriaceae (CPE) was isolated from a patient diagnosed with sigmoid colon diverticulitis. At first, laboratory testing suggested it was Klebsiella oxytoca or Pantoea sp.; however, a complete genome sequence of the isolate, MRY16-398, revealed that it could be novel species, most similar to [Kluyvera] intestini, of which taxonomic nomenclature is still under discussion. Orthologous conserved gene analysis among 42 related bacterial strains indicated that MRY16-398 was classified as the newly proposed genus Metakosakonia. Further, MRY16-398 was found to harbor the blaIMP-6 gene-positive class 1 integron (In722) in plasmid pMRY16-398_2 (IncN replicon, 47.4 kb in size). This finding implies that rare and opportunistic bacteria could be potential infectious agents. In conclusion, our results highlight the need for continuous monitoring for CPE even in nonpathogenic bacteria in the nosocomial environment.

Keywords: Kluyvera, blaIMP-6, carbapenemase, IncN, Metakosakonia

Received: 31 Jul 2018; Accepted: 06 Nov 2018.

Edited by:

Yi-Wei Tang, Memorial Sloan Kettering Cancer Center, United States

Reviewed by:

Filipa Grosso, Faculdade de Farmácia, Universidade do Porto, Portugal
Ghassan M. Matar, American University of Beirut, Lebanon  

Copyright: © 2018 Sekizuka, Matsui, Takahashi, Hayashi, Suzuki, Tokaji and Kuroda. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Makoto Kuroda, National Institute of Infectious Diseases (NIID), Tokyo, 162-8640, Tôkyô, Japan, makokuro@niid.go.jp