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Front. Microbiol. | doi: 10.3389/fmicb.2018.02858

A surface protein from Lactobacillus plantarum increases the adhesion of Lactobacillus strains to human epithelial cells

 Guangqiang Wang1, Minghui Zhang1, Yongjun Xia1, Phoency F.-H. Lai1 and  Lianzhong Ai1*
  • 1University of Shanghai for Science and Technology, China

Adhesion to epithelial cells is considered important for Lactobacillus to exert probiotic effects. In this study, we found that trypsin treatment decreased the adhesion ability of Lactobacillus plantarum AR326 and AR269, which exhibit good adhesion ability, and surface proteins extracts increased the adhesion of the strains with poor adhesion ability. By SDS-PAGE and mass spectrometry analysis, the main component of the surface proteins was detected and identified as a protein of approximately 37 kDa. It was 100% homologous with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from L. plantarum WCFS1. The adhesion of AR326 and AR269 was decreased significantly by blocking with the anti-GAPDH antibody, and GAPDH restored the adhesion of AR326 and AR269 treated with trypsin. In addition, purified GAPDH significantly increased the adhesion of the strains with poor adhesion ability. These results indicated that GAPDH mediates the adhesion of these highly adhesive lactobacilli to epithelial cells and can be used to improve the adhesion ability of probiotics or other bacteria of interest.

Keywords: Lactobacillus, surface protein, GAPDH, Adhesion, HT-29

Received: 15 Aug 2018; Accepted: 06 Nov 2018.

Edited by:

Chunbao Li, Nanjing Agricultural University, China

Reviewed by:

Armen Trchounian, Yerevan State University, Armenia
Zhihong Sun, Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education, Inner Mongolia Agricultural University, China
Ming Du, Dalian Polytechnic University, China  

Copyright: © 2018 Wang, Zhang, Xia, Lai and Ai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Lianzhong Ai, University of Shanghai for Science and Technology, Shanghai, China, ailianzhong@hotmail.com