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Front. Microbiol. | doi: 10.3389/fmicb.2018.03089

Alterations of Candidatus Liberibacter asiaticus-associated microbiota decrease survival of Ca. L. asiaticus in in vitro assays

Kazuki Fujiwara1, 2, Toru Iwanami2 and  Fujikawa Takashi2*
  • 1National Agriculture and Food Research Organization (NARO), Japan
  • 2National Agriculture and Food Research Organization (NARO), Japan

Phloem-inhabiting bacterial phytopathogens often have smaller genomes than other bacterial phytopathogens. It is thought that they depend on both other phloem microbiota and phloem nutrients for colonization of the host. However, the mechanism underlying associations between phloem-inhabiting phytopathogens and other phloem microbiota are poorly understood. Here, we demonstrate that the survival of Candidatus Liberibacter asiaticus (CLas), a cause of huanglongbing (citrus greening disease), depends on interplay with a specific subset of CLas-associated microbiota. CLas was not susceptible to oxytetracycline in vitro. However, oxytetracycline treatment eliminated a particular sub-community dominated by the Comamonadaceae, Flavobacteriaceae, Microbacteriaceae, and Pseudomonadaceae, decreasing CLas survival. We speculate that CLas uses ecological services derived from CLas-associated microbiota to colonize the host and to construct a pathogen-associated community that stimulates disease development.

Keywords: Candidatus Liberibacter asiaticus, Huanglongbing (HLB), Candidatus Liberibacter asiaticus-associated microbiota, rna sequence, Metagenomics, culture medium

Received: 02 Oct 2018; Accepted: 29 Nov 2018.

Edited by:

Brigitte Mauch-Mani, University of Neuchâtel, Switzerland

Reviewed by:

Lukasz L. Stelinski, University of Florida, United States
Siti -. Subandiyah, Gadjah Mada University, Indonesia  

Copyright: © 2018 Fujiwara, Iwanami and Takashi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Fujikawa Takashi, National Agriculture and Food Research Organization (NARO), Tsukuba, 305-8517, Ibaraki, Japan, ftakashi@affrc.go.jp