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Front. Microbiol. | doi: 10.3389/fmicb.2018.03125

Proteogenomic analysis of Epibacterium mobile BBCC367, a relevant marine bacterium isolated from the South Pacific Ocean

 Sabine Matallana Surget1*,  Johannes Werner2,  Ruddy Wattiez3, Karine Lebaron1,  Laurent INTERTAGLIA4, Callum Regan1, James Morris1, Hanno Teeling5,  Manuel Ferrer6,  Peter N. Golyshin7,  Dimitrios Gerogiorgis8, Simon I. Reilly8 and Philippe Lebaron4
  • 1University of Stirling, United Kingdom
  • 2Leibniz Institute for Baltic Sea Research (LG), Germany
  • 3University of Mons, Belgium
  • 4Université Pierre et Marie Curie, France
  • 5Max Planck Institute for Marine Microbiology (MPG), Germany
  • 6Spanish National Research Council (CSIC), Spain
  • 7Bangor University, United Kingdom
  • 8University of Edinburgh, United Kingdom

Epibacterium mobile BBCC367 is a marine bacterium that is common in coastal areas. It belongs to the Roseobacter clade, a widespread group in pelagic marine ecosystems. Species of the Roseobacter clade are regularly used as models to understand the evolution and physiological adaptability of generalist bacteria. We used gel-free shotgun proteomics to assess E. mobile BBCC367 protein expression under 16 different conditions, including stress factors such as elevated temperature, nutrient limitation, high metal concentration, and UVB exposure. Comparison of the different conditions allowed us not only to retrieve almost 70% of the predicted proteins, but also to define three main protein assemblages: 584 essential core proteins, 2,144 facultative accessory proteins and 355 specific unique proteins. Among these we studied a wide diversity of expressed protein functions, including transporters, DNA repair proteins, quorum sensing, transcriptional/translational regulators, and chemotaxis proteins that provided insights into how E. mobile BBCC367 adapts to environmental changes and copes with diverse forms of stress.

Keywords: Epibacterium mobile, proteogenomic, Roseobacter, Stress response and adaptation, Quantitative Proteomics

Received: 16 Sep 2018; Accepted: 03 Dec 2018.

Edited by:

David A. Walsh, Concordia University, Canada

Reviewed by:

Kai Tang, Xiamen University, China
Brook L. Nunn, University of Washington, United States  

Copyright: © 2018 Matallana Surget, Werner, Wattiez, Lebaron, INTERTAGLIA, Regan, Morris, Teeling, Ferrer, Golyshin, Gerogiorgis, Reilly and Lebaron. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Sabine Matallana Surget, University of Stirling, Stirling, United Kingdom, sabine.matallanasurget@stir.ac.uk