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Microbial Ecotoxicology

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Front. Microbiol. | doi: 10.3389/fmicb.2018.03169

Dynamics of Bacterial Communities Mediating the Treatment of an As-Rich Acid Mine Drainage in a Field Pilot

 Marina Hery1*, Elia Laroche2, 3, Lidia Fernandez-Rojo2, 4, Vincent Tardy2, 5,  Odile Bruneel2, Corinne Casiot2, Angélique Désoeuvre2, Fabienne Battaglia-Brunet3,  Catherine Joulian3 and Marina Héry2
  • 1Université de Montpellier, France
  • 2UMR5569 HydroSciences Montpellier (HSM), France
  • 3Département de Environnement et des Ecotechnologies, Bureau de Recherches Géologiques et Minières, France
  • 4Instituto de Diagnóstico Ambiental y Estudios del Agua (IDAEA), Spain
  • 5Unité de Recherche Riverly, IRSTEA, France

Passive treatment based on iron biological oxidation is a promising strategy for As-rich acid mine drainage (AMD) remediation. In the present study, we characterized (by 16S rRNA metabarcoding) the bacterial diversity in a field-pilot bioreactor treating extremely As-rich AMD in situ, over a six months monitoring period. Inside the bioreactor, the bacterial communities responsible for iron and arsenic removal formed a biofilm (“biogenic precipitate”) whose composition varied in time and space. These communities evolved throughout time from a structure at first similar to the one of the feed water used as an inoculum to a structure quite similar to the natural biofilm developing in situ in the AMD. Over the whole monitoring period, iron oxidizing bacteria always largely dominated the biogenic precipitate, with distinct populations (Gallionella, Ferrovum, Leptospirillum, Acidithiobacillus, Ferritrophicum), whose relative proportions extensively varied among time and space. A spatial structuration was observed inside the trays (arranged in series) composing the bioreactor. This spatial dynamic could be linked to the variation of the physico-chemistry of the AMD water between the raw water entering and the treated water exiting the pilot. According to redundancy analysis (RDA), the following parameters exerted a control on the whole bacterial communities potentially involved in the water treatment process: dissolved oxygen, temperature, pH, dissolved sulfates, arsenic and Fe(II) concentrations and redox potential. Appreciable arsenite oxidation occurring in the bioreactor could be linked to the stable presence of two distinct monophylogenetic groups of Thiomonas related bacteria. The ubiquity and the physiological diversity of the bacteria identified, as well as the presence of bacteria of biotechnological relevance, suggested that this treatment system could be applied to the treatment of other AMD.

Keywords: Acid mine drainage (AMD), Arsenic, Bioremed iation, Eco-engineering, Iron-oxidising bacteria, arsenite-oxidizing bacteria, microbial ecotoxicology

Received: 12 Sep 2018; Accepted: 07 Dec 2018.

Edited by:

Ghiglione Jean-Francois, Center for the National Scientific Research (CNRS), France

Reviewed by:

Steven Singer, Lawrence Berkeley National Laboratory (LBNL), United States
Jonathan D. Van Hamme, Thompson Rivers University, Canada  

Copyright: © 2018 Hery, Laroche, Fernandez-Rojo, Tardy, Bruneel, Casiot, Désoeuvre, Battaglia-Brunet, Joulian and Héry. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Marina Hery, Université de Montpellier, Montpellier, 34090, Languedoc-Roussillon, France, marina.hery@univ-montp2.fr