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Front. Microbiol. | doi: 10.3389/fmicb.2019.00534

A novel highly efficient device for growing micro-aerophilic microorganisms

Maxime FUDUCHE1, Sylvain DAVIDSON1,  Céline BOILEAU1,  Long-Fei Wu2 and  Yannick COMBET-BLANC1*
  • 1Institut de recherche pour le développement (IRD), France
  • 2UMR7283 Laboratoire de chimie bactérienne (LCB), France

This work describes a novel, simple, and cost-effective culture system, named Micro-Oxygenated Culture Device (MOCD), designed to grow microorganisms under particularly challenging oxygenation conditions. Two microaerophilic magnetotactic bacteria, a freshwater Magnetospirillum gryphiswaldense strain MSR-1 and a marine Magnetospira sp. strain QH-2, were used as biological models to prove the Micro-Oxygen Culture Device (MOCD) efficiency and to evaluate its specifications. MSR-1 and QH-2 growth rates with the MOCD were respectively increased by 4.0 and 2.0-fold, when compared to the traditional growing techniques using simple bottles. Moreover, the MOCD, which is far less sophisticated than the oxystat-bioreactors specifically designed to control the low O2 concentrations, was proven to be similarly efficient for the MSR-1 and QH-2 cultures regarding growth rate, and even better for MSR-1 regarding the cell yield (rise of 70% rise). Thus, the wide range of oxygenation conditions, including the different O2-gradients, provided by MOCD makes it an innovative and ingenious culture device that opens new perspectives to grow microaerobic microorganisms.

Keywords: Microaerobes, MOCD, magnetotactic bacteria, Magnetospirillum gryphiswaldense MSR-1, Magnetospira sp. QH- 2

Received: 02 Oct 2018; Accepted: 01 Mar 2019.

Edited by:

Philippe M. Oger, UMR5240 Microbiologie, Adaptation et Pathogenie (MAP), France

Reviewed by:

Beverly E. Flood, University of Minnesota Twin Cities, United States
Feth-el-Zahar Haichar, Microbial Ecology, France  

Copyright: © 2019 FUDUCHE, DAVIDSON, BOILEAU, Wu and COMBET-BLANC. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Yannick COMBET-BLANC, Institut de recherche pour le développement (IRD), Marseille, Provence-Alpes-Côte d'Azur, France, yannick.combet-blanc@mio.osupytheas.fr