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Front. Microbiol. | doi: 10.3389/fmicb.2019.01159

Transcriptome analysis reveals AI-2 relevant genes of multi-drug resistant Klebsiella pneumoniae in response to eugenol at sub-MIC

Yi-ming Wang1, Wen-long Dong1, Odah Kokouayefounin1,  Ling-cong Kong1* and  Hong-xia Ma1*
  • 1College of Animal Science and Technology, Jilin Agricultural University, China

Eugenol, the major active essential oil component of clove, was reported possessing QS(quorum sensing) inhibitory activity. Previous study found that eugenol could bind to quorum sensing receptors of P.aeruginosa and down-regulate the expression of S.mutans virulence genes at sub-MIC(minimum inhibitory concentration) without affecting the bacterial growth. However, the alterations of QS signal molecules in K.pneumoniae at transcription levels was not well understood. To better understanding interactions of K.pneumoniae in response to eugenol and explore molecular regulations, transcriptome sequencing was performed. a total of 5779 differentially expressed genes (DEGs) enriched in a variety of biological processes and pathways were identified. The transcriptional data were validated by qPCR and the results showed that the expression profile of 4 major genes involved in autoinducers-2 (AI-2) synthesis, including luxS, pfs and lsrK were consistant with transcriptome analysis except for lsrR, a transcriptional repressor gene of lsr operon, which we speculate would repress the expression of following genes responsible for AI-2 signal transmission in vivo. In vitro AI-2 synthesis assay also revealed that eugenol could inhibit AI-2 generation. The results of our study offers insights into the mechanism of QS inhibitory activity and K.pneumoniae AI-2 alterations after treatment of eugenol.

Keywords: Eugenol, K.pneumoniae, Quorum Sensing, transcriptome analysis, AI-2 synthesis, Inhibitory effect

Received: 07 Nov 2018; Accepted: 07 May 2019.

Edited by:

Hua-Bin Li, Sun Yat-sen University, China

Reviewed by:

Jianfeng Wang, Jilin University, China
Tommaso Felicetti, Dipartimento Di Scienze Farmaceutiche, Università Degli Studi Di Perugia, Italy  

Copyright: © 2019 Wang, Dong, Kokouayefounin, Kong and Ma. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Ling-cong Kong, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, China, congwbs@126.com
Prof. Hong-xia Ma, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, China, hongxia0731001@163.com