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Front. Microbiol. | doi: 10.3389/fmicb.2019.01208

Comparative Transcriptomics and Proteomics of Atractylodes lancea in Response to Endophytic Fungus Gilmaniella sp. AL12 Reveals Regulation in Plant Metabolism

Jie Yuan1, Wei Zhang1,  Kai Sun1, Meng-Jun Tang1, Piao-Xue Chen1,  Xia Li2 and  Chuan-Chao Dai3*
  • 1College of Life Science, Nanjing Normal University, China
  • 2Food Crop Research Institute, Jiangsu Academy of Agricultural Sciences, China
  • 3Nanjing Normal University, China

The fungal endophyte Gilmaniella sp. AL12 can establish a beneficial association with the medicinal herb Atractylodes lancea, and improve plant growth and sesquiterpenoids accumulation, which is termed “double promotion”. Our previous studies have uncovered the underling primary mechanism based on some physiological evidences. However, a global understanding of gene or protein expression regulation in primary and secondary metabolism and related regulatory processes is still lacking. In this study, we employed transcriptomics and proteomics of Gilmaniella sp. AL12-inoculated and Gilmaniella sp. AL12-free plants to study the impact of endophyte inoculation at the transcriptional and translational levels. The results showed that plant genes involved in plant immunity and signaling were suppressed, similar to the plant response caused by some endophytic fungi and biotroph pathogen. The downregulated plant immunity may contribute to plant-endophyte beneficial interaction. Additionally, genes and proteins related to primary metabolism (carbon fixation, carbohydrate metabolism and energy metabolism) tended to be upregulated after Gilmaniella sp. AL12 inoculation, which was consistent with our previous physiological evidences. And, Gilmaniella sp. AL12 upregulated genes involved in terpene skeleton biosynthesis, and upregulated genes annotated as β-farnesene synthase and β-caryophyllene synthase. Based on the above results, we proposed that endophyte-plant associations may improve production (biomass and sesquiterpenoids accumulation) by increasing the source (photosynthesis), expanding the sink (glycolysis and tricarboxylic acid cycle), and enhancing the metabolic flux (sesquiterpenoids biosynthesis pathway) in Atractylodes lancea. And, this study will help to further clarify plant-endophyte interactions.

Keywords: Atractylodes lancea, endophytic fungi, Plant Immunity, Metabolism, Terpenoid biosynthesis, Beneficial interaction

Received: 14 Mar 2019; Accepted: 13 May 2019.

Edited by:

Marco Catoni, University of Birmingham, United Kingdom

Reviewed by:

Khondoker M. Dastogeer, Tokyo University of Agriculture and Technology, Japan
Stefania Daghino, University of Turin, Italy  

Copyright: © 2019 Yuan, Zhang, Sun, Tang, Chen, Li and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Chuan-Chao Dai, Nanjing Normal University, Nanjing, China, daichuanchao@njnu.edu.cn