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Front. Microbiol. | doi: 10.3389/fmicb.2019.01443

Role of LptD in resistance to glutaraldehyde and pathogenicity in Riemerella anatipestifer

 Li Huang1, 2, 3,  Ming-Shu Wang1, 2, 3, Ting Mo1, 2, 3,  Ma-Feng Liu1, 2, 3, Francis Biville4,  De-Kang Zhu2, 3, Ren-Yong Jia1, 2, 3,  Shun Chen1, 2, 3, Xin-Xin Zhao1, 2, 3, Qiao Yang1, 2, 3, Ying Wu1, 2, 3, Sha-Qiu Zhang1, 2, 3, Juan Huang1, 2, 3,  Bin Tian1, 2, 3, Yun-Ya Liu1, 2, 3, Ling Zhang2, 3, Yan-Ling Yu2, 3, Lei-Chang Pan1, 2, 3,  Mujeeb U. Rehman1, 2, 3,  Xiao-Yue Chen2, 3 and  An-Chun Cheng1, 2, 3*
  • 1Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, China
  • 2Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, China
  • 3Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, China
  • 4Institut Pasteur, France

Riemerella anatipestifer (R. anatipestifer, RA) is a multidrug-resistant, gram-negative bacterium that causes disease in ducks and other birds. Despite being an important pathogen in the poultry industry, the pathogenesis and drug resistance mechanisms of this bacterium are poorly understood. An analysis of our unpublished RNA-Seq data showed that lptD is transcribed at higher levels in RA CH-1 strain than in RA ATCC11845 strain. In addition, RA CH-1 has been shown to display more extensive drug resistance than RA ATCC11845. Since LptD is involved in LPS biogenesis and drug resistance, we wondered if lptD is associated with increased R. anatipestifer resistance to glutaraldehyde, a disinfectant used in the poultry industry. In this study, the MIC of glutaraldehyde for RA CH-1 was determined to be 0.125%, whereas an MIC of 0.05% was observed for RA ATCC11845. Furthermore, the level of lptD transcription in RA CH-1 was consistently 2-fold higher than that observed in RA ATCC11845. Moreover, lptD transcription was upregulated in both strains at a subinhibitory concentration of glutaraldehyde. The role of lptD in R. anatipestifer was further assessed by constructing an RA ATCC11845 strain with low lptD expression, RA ATCC11845 lptD-. The growth of RA ATCC11845 lptD- was severely impaired, and this strain was more sensitive than the wild-type strain to glutaraldehyde. Moreover, compared to the wild-type strain, RA ATCC11845 lptD- exhibited decreased biofilm formation and was more sensitive to duck serum. Finally, low lptD expression led to decreased colonization in ducklings. These results suggest that lptD is involved in R. anatipestifer glutaraldehyde resistance and pathogenicity.

Keywords: Riemerella anatipestifer, lipopolysaccharide (LPS), LptD, hydrophobic drug resistance, Membrane Permeability

Received: 10 Mar 2019; Accepted: 07 Jun 2019.

Edited by:

Xian-Zhi Li, Health Canada, Canada

Reviewed by:

Francesco Imperi, Department of Science, Roma Tre University, Italy
Paola Sperandeo, University of Milan, Italy  

Copyright: © 2019 Huang, Wang, Mo, Liu, Biville, Zhu, Jia, Chen, Zhao, Yang, Wu, Zhang, Huang, Tian, Liu, Zhang, Yu, Pan, Rehman, Chen and Cheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. An-Chun Cheng, Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 625014, Sichuan Province, China, chenganchun@vip.163.com