Comparison of PCR and viable count as a method for enumeration of bacteria in an AJ mouse aerosol model of Q Fever
- 1Defence Science and Technology Laboratory, United Kingdom
- 2University of Exeter, United Kingdom
Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonisation and dissemination of other bacterial infections in animal models is tracked using viable counts, enabling an accurate assessment of bacterial load within tissues. Following recent advances in the culture methods for C.burnetii, with the development of ACCM-2, it has become possible to monitor the pathogenesis of C. burnetii in a host by direct culture. Here we compare and contrast the different information gained by using PCR or viable counts to study this disease.
Keywords: Coxiella burneti, mouse model, Bacterial viability/culturability, PCR, Q Fever
Received: 20 Feb 2019;
Accepted: 21 Jun 2019.
Edited by:Ying Zhang, Johns Hopkins University, United States
Reviewed by:Stacey Gilk, School of Medicine, Indiana University Bloomington, United States
Eric Ghigo, IHU Mediterranee Infection, France
Anders Omsland, Washington State University, United States
Gilbert Kersh, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention (CDC), United States
Copyright: © 2019 Hartley, Ralph, Norville, Prior and Atkins. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Margaret G. Hartley, Defence Science and Technology Laboratory, Salisbury, United Kingdom, firstname.lastname@example.org