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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01567

Evaluation of bile salt hydrolases, cholesterol-lowering capabilities and probiotic potential of Enterococcus faecium isolated from rhizosphere

  • 1University of Delhi, India

Bile salt hydrolase (BSH) activity, hypo-cholesterolemic effect and probiotic properties have been reported for Enterococcus strains isolated from animal- and human-gut and, fermented foods but not for strains isolated from environmental niches, like aquatic and terrestrial plants, soil and water. The present study is the first report on isolation of Enterococcus faecium from rhizospheric soils which harbor bsh gene, remove cholesterol in-vitro and possess essential and desirable probiotic attributes. Fifteen samples were collected from different sites located in northern, southern and central regions of India, of which five yielded pure colonies which were named as LR2, LR3, ER5, LR13 and VB1. These were identified by 16S rRNA gene sequencing as E. faecium and evaluated for BSH activity, cholesterol-lowering potential in-vitro and probiotic properties. Our results indicated that all the strains were capable of surviving in harsh conditions of gastro-intestinal tract and did not harbor any of the virulence genes. Though, all strains showed the presence of bsh and potential for cholesterol removal, E. faecium strain LR13 showed a remarkable cholesterol removal capability, vancomycin susceptibility and possessed most of the desirable and essential attributes of a probiotic. Hence, it seems to be fairly promising probiotic candidate which needs to be further evaluated in in vivo studies, especially for hypo-cholesterolemic potential.

Keywords: Bile salt hydrolase (BSH), Enterococcus faecium, Probiotics, hypo-cholesterolemia, Cholesterol-lowering

Received: 15 Mar 2019; Accepted: 24 Jun 2019.

Edited by:

Haifeng Zhao, South China University of Technology, China

Reviewed by:

Jorge Reinheimer, National University of the Littoral, Argentina
YOUSEF NAMI, Agricultural Biotechnology Research Institute of Iran, Iran
Choi Ki Choon, NIAS  

Copyright: © 2019 Singhal, Maurya, Mohanty, Kumar and Virdi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Jugsharan S. Virdi, University of Delhi, New Delhi, 110007, National Capital Territory of Delhi, India,