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Front. Microbiol. | doi: 10.3389/fmicb.2019.01597

Negative plant-soil feedback driven by re-assemblage of the rhizosphere microbiome with the growth of Panax notoginseng

 Shusheng Zhu1*,  Luoli Fen1,  Cunwu Guo1,  Tao L. Wang1, Junxing Zhang1, Linmei Deng1, Kaifeng Luo1,  Liu Y. Xiang1,  Huichuan Huang1,  Xinyue Mei1 and  Min Yang1*
  • 1Yunnan Agricultural University, China

There is a concerted understanding of the accumulation of soil pathogens as the major driving factor of negative plant-soil feedback (NPSF). However, our knowledge of the connection between plant growth, pathogen build-up and soil microbiome assemblage is limited. In this study, significant negative feedback between the soil and sanqi (Panax notoginseng) was found, which were caused by the build-up of the soil-borne pathogens Fusarium oxysporum, F. solani, and Monographella cucumerina. Soil microbiome analysis revealed that the rhizospheric fungal and bacterial communities were changed with the growth of sanqi. Deep analysis of the phylum and genus levels corroborated that rhizospheric fungal Ascomycota, including the soil-borne pathogens F. oxysporum, F. solani, and especially M. cucumerina, were significantly enriched with the growth of sanqi. However, the bacteria Firmicutes and Acidobacteria, including the genera Pseudomonas, Bacillus, Acinetobacter and Burkholderia, were significantly suppressed with the growth of sanqi. Using microbial isolation and in vitro dual culture tests, we found that most isolates derived from the suppressed bacterial genera showed strong antagonistic ability against the growth of sanqi soil-borne pathogens. Interestingly, inoculation of these suppressed isolates in consecutively cultivated soil could significantly alleviate NPSF. In summary, sanqi growth can suppress antagonistic bacteria through re-assemblage of the rhizosphere microbiome and cause the accumulation of soil-borne pathogens, eventually building negative feedback loops between the soil and plants.

Keywords: Panax notoginseng (Burk) F. H. Chen, microbiome, soil-borne pathogens, rhizosphere, Negative plant-soil feedback

Received: 15 Mar 2019; Accepted: 26 Jun 2019.

Edited by:

Brigitte Mauch-Mani, Université de Neuchâtel, Switzerland

Reviewed by:

Raza Waseem, Nanjing Agricultural University, China
Zongzhuan Shen, Nanjing Agricultural University, China  

Copyright: © 2019 Zhu, Fen, Guo, Wang, Zhang, Deng, Luo, Xiang, Huang, Mei and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Shusheng Zhu, Yunnan Agricultural University, Kunming, China, shushengzhu79@126.com
Dr. Min Yang, Yunnan Agricultural University, Kunming, China, yangminscnc@126.com