Impact Factor 4.259 | CiteScore 4.30
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01639

Isolation and Characterization of Halotolerant Plant Growth Promoting Rhizobacteria from Durum Wheat (Triticum turgidum subsp. durum) Cultivated in Saline Areas of the Dead Sea Region

  • 1Department of Biological Sciences, School of Science, University of Jordan, Jordan
  • 2University of Jordan, Jordan
  • 3Department of Horticulture and Crop Science, School of Agriculture, University of Jordan, Jordan
  • 4Department of Plant Protection, School of Agriculture, University of Jordan, Jordan
  • 5Shoubak University College, Al-Balqa Applied University, Jordan

Plant growth promoting rhizobacteria (PGPR) are beneficial microorganisms that can be utilized to improve plant responses against biotic and abiotic stresses. In this study, 74 halotolerant bacterial isolates were isolated from rhizosphere and endorhizosphere of durum wheat (Triticum turgidum subsp. durum) plants cultivated in saline environments in the Ghor region near the east of the Dead Sea. 16S rDNA partial sequences and phylogenetic analysis of 62 isolates showed clear clustering of the isolates into three phyla: Firmicutes (61.3%), Proteobacteria (29.0%) and Actinobacteria (9.7%). At the genus level, the majority of them were grouped within the Bacillus, Oceanobacillus and Halomonas genera. The isolates, which possessed plant growth promoting traits including nitrogen fixation, ACC deaminase activity, auxin production, inorganic phosphate solubilization and siderophore production, were selected. The effect of the inoculation of selected PGPR strains on growth of salt sensitive and salt tolerant durum wheat genotypes under high salt stress conditions was evaluated. Six halotolerant PGPR strains were able to improve survival in inoculated plants under high salinity stress conditions as reflected in higher germination percentages and seedling root growth when compared with non-inoculated plants. Furthermore, three halotolerant PGPR strains were able to improve durum wheat tolerance to water deficit stress. In addition, antagonistic effect in four halotolerant PGPR strains against an aggressive pathogenic isolate of Fusarium culmorum that causes crown rot disease was observed in a dual culture assay. In conclusion, the halotolerant PGPR strains described in this study might have great potential to improve durum wheat productivity under different stress conditions.

Keywords: ACC deaminase, durum wheat, endophyte, Halotolerant PGPR, Salinity

Received: 25 Aug 2018; Accepted: 02 Jul 2019.

Edited by:

Youssef Rouphael, University of Naples Federico II, Italy

Reviewed by:

Zisis Vryzas, Democritus University of Thrace, Greece
Mariateresa Cardarelli, Council for Agricultural and Economics Research, Italy  

Copyright: © 2019 Albdaiwi, Khaymi-Horani, Ayad, Kholoud M. Alananbeh and Al-Sayaydeh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Hala Khaymi-Horani, University of Jordan, Amman, Jordan, horani-h@ju.edu.jo