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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01850

Disease specific bacterial communities in a coralline algae of the northwestern Mediterranean Sea: a combined culture dependent and -independent approach

  • 1Institut de recherche pour le développement (IRD), New Caledonia
  • 2UMS2348 Observatoire océanologique de Banyuls-sur-Mer (OOB), France
  • 3UMR9220 Ecologie marine tropicale dans les Océans Pacifique et Indien (ENTROPIE), France
  • 4UMR8222 Laboratoire d'Ecogéochimie des Environnements Benthiques (LECOB), France

Crustose coralline red algae (CCA) are important components of marine ecosystems thriving from tropical waters and up to the poles. They fulfill important ecological services including framework building and induction of larval settlement. Like other marine organisms, CCAs have not been spared by the increase in marine disease outbreaks. The white-band syndrome has been recently observed in corallines from the Mediterranean Sea indicating that the disease threat has extended from tropical to temperate waters. Here, we examined the microbiome and the pathobiome of healthy and diseased Neogoniolithon brassica-florida coralline algae in the Mediterranean Sea by combining culture-dependent and -independent approaches. The coralline white-band syndrome was associated with a distinct pathobiome compared to healthy tissues and showed similarities with the white-band syndrome described in the Caribbean Sea. A sequence related to the genus Hoeflea, order Rhizobiales, characterised the white-band disease pathobiome described by amplicon sequencing. No representative of this genus was isolated by culture. We, however, successfully isolated an abundant member of the healthy CCA microbiome, an Alphaproteobateria of the family Rhodobacteraceae. In conclusion, we did not identify a potential causative agent of the disease, but through the complementarity culture dependent and independent approaches we characterised the healthy microbiome of the coralline and the possible opportunistic bacteria colonizing diseased tissues.

Keywords: Crustose coralline algae (CCA), Disease, Bacterial communities, pathobiome, culture, NGS (Next Generation Sequencing)

Received: 15 Mar 2019; Accepted: 26 Jul 2019.

Edited by:

Russell T. Hill, Institute of Marine and Environmental Technology, University of Maryland, Baltimore County, United States

Reviewed by:

Marco J. Coolen, Curtin University, Australia
Javier Del Campo, Rosenstiel School of Marine and Atmospheric Science, University of Miami, United States  

Copyright: © 2019 Quere, INTERTAGLIA, PAYRI and Galand. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Gaelle Quere, Institut de recherche pour le développement (IRD), Noumea, New Caledonia, queregaelle@gmail.com