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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01936

High Genetic Diversity and Species Complexity of Diaporthe Associated with Grapevine Dieback in China

  • 1Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, China
  • 2Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, China
  • 3Department of Biology, McMaster University, Canada
  • 4College of Plant Protection, China Agricultural University, China
  • 5Center of Excellence in Fungal Research, Mae Fah Luang University, Thailand

Grapevine trunk diseases have become one of the main threats to grape production worldwide, with Diaporthe species as an emerging group of pathogens in China. At present, relatively little is known about the taxonomy and genetic diversity of Chinese Diaporthe populations, including their relationships to other populations worldwide. Here, we conducted an extensive field survey in six provinces in China to identify and characterize Diaporthe species in grape vineyards. Ninety-four isolates were identified and analyzed using multi-locus phylogeny. The isolates belonged to eight species, including three novel taxa, D. guangxiensis, D. hubeiensis, D. viniferae, and three new host records, D. gulyae D. pescicola and D. unshiuensis. The most commonly isolated species was D. eres. In addition, high genetic diversity was observed for D. eres in Chinese vineyards. Haplotype network analysis of D. eres isolates from China and Europe showed a close relationship between samples from the two geographical locations and evidence for recombination. In comparative pathogenicity testing, D. gulyae was the most aggressive taxon, whereas D. hubeiensis was the least aggressive. This study provides new insights into the Diaporthe species associated with grapevines in China, and our results can be used to develop effective disease management strategies.

Keywords: Novel species, new host record, Network analysis, Phylogeography, Phomopsis.

Received: 21 May 2019; Accepted: 06 Aug 2019.

Copyright: © 2019 Manawasinghe, Dissanayake, Li, Liu, Wanasinghe, Xu, Zhao, Wei, Zhou, Hyde, Brooks and Yan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Jiye Yan, Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China, jiyeyan@vip.163.com