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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01950

Putrescine is an intraspecies and interkingdom cell-cell communication signal modulating the virulence of Dickeya zeae

 Zurong Shi1, Qingwei Wang1, Yasheng Li1,  Zhibin Liang1,  XU L. HUI1, Jianuan Zhou1,  Zi-Ning Cui1 and  Lian-Hui Zhang1*
  • 1Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University, China

The infections caused by Dickeya zeae become a severe problem in recent year, but the regulation mechanisms that govern the bacterial virulence remain to be fragmental. Here we report the investigation of potential involvement of polyamines in regulation of D. zeae virulence. We showed that null mutation of speA encoding arginine decarboxylase dramatically decreased swimming motility, swarming motility and biofilm formation, and exogenous addition of putrescine effectively rescues the defective phenotypes of D. zeae. HPLC and mass spectrometry analysis validated that SpeA was essential for production of putrescine in D. zeae. In addition, we demonstrated that D. zeae EC1 could detect and response to putrescine molecules produced by itself or from host plant through specific transporters. Among the two transporters characterized, the one represented by PotF played a dominated role over the other represented by PlaP in modulation of putrescine-dependent biological functions. Furthermore, we provided evidence that putrescine signal is critical for D. zeae EC1 bacterial invasion and virulence against rice seeds. Our data depict a novel function of putrescine signal in pathogen-host communication and in modulation of the virulence of an important plant bacterial pathogen.

Keywords: Putrescine, cell-cell signaling, pathogen-host communication, Polyamine, Regulatory elements

Received: 15 Jun 2019; Accepted: 08 Aug 2019.

Edited by:

Brad Day, Michigan State University, United States

Reviewed by:

Matias Pasquali, University of Milan, Italy
Sébastien Bontemps-Gallo, Institut Pasteur de Lille, France
Malgorzata Golanowska, Independent researcher, Netherlands  

Copyright: © 2019 Shi, Wang, Li, Liang, HUI, Zhou, Cui and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Lian-Hui Zhang, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University, Guangzhou, China, lhzhang01@scau.edu.cn