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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.01990

Boosting heterologous phenazine production in Pseudomonas putida KT2440 through the exploration of the natural sequence space

  • 1Institute of Applied Microbiology, RWTH Aachen University, Germany
  • 2Leibniz Institute for Natural Product Research and Infection Biology, Germany
  • 3Faculty of Biological Sciences, Friedrich Schiller University Jena, Germany

Phenazine-1-carboxylic acid (PCA) and its derivative pyocyanin (PYO) are natural redox mediators in bioelectrochemical systems and have the potential to enable new bioelectrochemical production strategies. The native producer Pseudomonas aeruginosa harbors two identically structured operons in its genome, which encode the enzymes responsible for PCA synthesis (phzA1-G1 (operon 1), phzA2-G2 (operon 2)). To optimize heterologous phenazines production in the biotech host Pseudomonas putida KT2440, we compared PCA production from both operons originating from P. aeruginosa strain PAO1 (O1.phz1 and O1.phz2) as well as from P. aeruginosa strain PA14 (14.phz1 and 14.phz2). Comparisons of phenazine synthesis and bioelectrochemical activity were performed between heterologous constructs with and without the combination with the genes phzM and phzS required to convert PCA to PYO. Despite a high amino acid homology of all enzymes of more than 97 %, P. putida harboring 14.phz2 produced 4-times higher PCA concentrations (80 μg/mL), which resulted in 3-times higher current densities (12 µA/cm2) compared to P. putida 14.phz1. The respective PCA/PYO producer containing the 14.phz2 operon was the best strain with 80 μg/mL PCA, 11 μg/mL PYO, and 22 µA/cm2 current density. Tailoring phenazine production also resulted in improved oxygen-limited metabolic activity of the bacterium through enhanced anodic electron discharge. To elucidate the reason for this superior performance, a detailed structure comparison of the PCA-synthesizing proteins has been performed. The here presented characterization and optimization of these new strains will be useful to improve electroactivity in P. putida for oxygen-limited biocatalysis.

Keywords: Pseudomonas putida, Phenazines, Phenazine 1-carboxylic acid, pyocyanin, Bioelectrochemical system (BES), heterologous production

Received: 20 Jun 2019; Accepted: 13 Aug 2019.

Edited by:

Amelia-Elena Rotaru, University of Southern Denmark, Denmark

Reviewed by:

Satoshi Kawaichi, University of Akron, United States
Alessandro Carmona, Other, Spain  

Copyright: © 2019 Askitosari, Boto, Blank and Rosenbaum. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Miriam A. Rosenbaum, Leibniz Institute for Natural Product Research and Infection Biology, Jena, 07745, Thuringia, Germany, miriam.rosenbaum@leibniz-hki.de