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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02002

Emergence of IncX3 plasmid-harbouring blaNDM-5 dominated by Escherichia coli ST48 in a goose farm in Jiangsu, China

 Ziyi Liu1, Xia Xiao1, Yan Li1, Yuan Liu1,  Ruichao Li1* and  Zhi-Qiang Wang1*
  • 1College of Veterinary Medicine, Yangzhou University, China

Twelve carbapenem-resistant E. coli strains were obtained from goose farms in Jiangsu, China. These isolates were resistant to multiple antimicrobials, and positive for the blaNDM-5. The carbapenem-resistance of all strains mediated by blaNDM-5 were successfully conjugated to E. coli J53. S1-PFGE and WGS results showed blaNDM-5 was located on IncX3 conjugative plasmids with a size of ca. 46kb. All blaNDM-5-bearing IncX3 plasmids shared the same genetic context almost identical to pNDM_MGR194-blaNDM-5 and pNDM-QD28-blaNDM-5 reported in India and China, respectively. The twelve strains belonged to three STs, in which the dominant type of E. coli isolated from breeding goose farm carrying blaNDM-5 was ST48. The emergence of blaNDM-5-bearing strains in goose farms and the clonal transmission of E. coli within the breeding goose farm highlighted the potential reservoir of carbapenemase genes in waterfowl farming system, which may further contaminate environments and pose a threat to public health. Comprehensive surveillance of carbapenem-resistant bacteria in goose farms warrants further study to evaluate the underlying risks.

Keywords: Carbapenemase genes, blaNDM-5, E. coli, Long-read sequencing, Plasmids

Received: 01 Jul 2019; Accepted: 15 Aug 2019.

Edited by:

Miklos Fuzi, Semmelweis University, Hungary

Reviewed by:

Hanna E. Sidjabat, University of Queensland, Australia
Pak-Leung Ho, The University of Hong Kong, Hong Kong  

Copyright: © 2019 Liu, Xiao, Li, Liu, Li and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Ruichao Li, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu Province, China, rchl88@yeah.net
Prof. Zhi-Qiang Wang, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu Province, China, zqwang@yzu.edu.cn