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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02006

Gga-miR-19b-3p inhibits Newcastle disease virus replication by suppressing inflammatory response via targeting RNF11 and ZMYND11

 Yu Chen1, Wen Liu1, Haixu Xu1, Jingjing Liu1, Yonghuan Deng1, Hao Cheng1,  Tiansong Zhan1, Xiaolong Lu1, Tianxing Liao1, Lili Guo1, Shanshan Zhu1, Yuru Pei1, Jiao Hu1, 2, Zenglei Hu1, Xiaowen Liu1, 2,  Xiaoquan Wang1, Min Gu1, 3, Shunlin Hu1 and  Xiufan Liu1, 2, 3*
  • 1Yangzhou University, China
  • 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, China
  • 3Jiangsu Key Laboratory of Zoonosis, Yangzhou University, China

Newcastle disease (ND), an acute and highly contagious avian disease caused by virulent Newcastle disease virus (NDV), often results in severe economic losses worldwide every year. Although it is clear that microRNAs (miRNAs) are implicated in modulating innate immune response to invading microbial pathogens, their role in host defense against NDV infection remains largely unknown. Our prior study indicates that gga-miR-19b-3p is up-regulated in NDV-infected DF-1 cells (a chicken embryo fibroblast cell line) and functions to suppress NDV replication. Here we report that overexpression of gga-miR-19b-3p promoted the production of NDV-induced inflammatory cytokines and suppressed NDV replication, whereas inhibition of endogenous gga-miR-19b-3p expression had an opposite effect. Dual-luciferase and gene expression array analyses revealed that gga-miR-19b-3p directly targets the mRNAs of ring finger protein 11 (RNF11) and zinc-finger protein, MYND-type containing 11 (ZMYND11), two negative regulators of nuclear factor kappa B (NF-κB) signaling, in DF-1 cells. RNF11 and ZMYND11 silencing by small interfering RNA (siRNA) induced NF-κB activity and inflammatory cytokine production, and suppressed NDV replication; whereas ectopic expression of these two proteins exhibited an opposite effect. Our study provides evidence that gga-miR-19b-3p activates NF-κB signaling by targeting RNF11 and ZMYND11, and that enhanced inflammatory cytokine production is likely responsible for the suppression of NDV replication.

Keywords: NDV, gga-miR-19b-3p, DF-1 cells, RNF11, ZMYND11, inflammatory cytokine, NF-κB signaling

Received: 23 May 2019; Accepted: 15 Aug 2019.

Copyright: © 2019 Chen, Liu, Xu, Liu, Deng, Cheng, Zhan, Lu, Liao, Guo, Zhu, Pei, Hu, Hu, Liu, Wang, Gu, Hu and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xiufan Liu, Yangzhou University, Yangzhou, China, xfliu@yzu.edu.cn