Original Research ARTICLE
T cell receptor immune repertoires are promptly reconstituted after methicillin-resistant Staphylococcus aureus infection
- 1Department of Orthodontics, The Affiliated Hospital of Qingdao University, China
- 2Department of Central Laboratory, Affiliated Hospital of Qingdao University, China
- 3Department of Basic Medical Sciences, College of Medicine, Xiamen University, China
- 4No 401 hospital of People's Liberation Army of China, China
T cells represent a subset of lymphocytes characterized by immunosurveillance and immunoregulation function. Peripheral blood mononuclear cells (PBMCs) are enriched in T cells, which exert critical antimicrobial roles in infectious diseases. High-throughput sequencing of the T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Flow cytometry was used to analyse the distribution of αβ/γδ T cells and their CD69, IFN-γ/IL-17 expression from PBMCs. Here, we utilized next-generation sequencing (NGS) to detect the complementarity determining region 3 (CDR3) of TCRβ (TRB) and TCRδ (TRD) chain after methicillin-resistant Staphylococcus aureus (MRSA) infection. Our data demonstrated a significant increase in the activation of αβ and γδ T cells after MRSA infection. Simultaneously, significantly high CDR3 amino acid (AA) diversity and markedly reconstituted TCR immune repertoires were observed after MRSA infection. Finally, we identified several MRSA-specific initial CDR3 AA motifs after MRSA infection. Our work reveals the profiles of TRB and TRD immune repertoires in response to MRSA and demonstrates a reconstitution of the TCR immune repertoire after MRSA infection.
Keywords: T cell receptor, immune repertoire, Methicillin-Resistant Staphylococcus aureus, Next-generation sequencing, Complementarity determining region 3
Received: 11 May 2019;
Accepted: 16 Aug 2019.
Copyright: © 2019 Liu, Liu, Zhu, Sun, Liang, Zhu and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mx. Kejia Wang, Department of Basic Medical Sciences, College of Medicine, Xiamen University, Xiamen, 361102, Fujian Province, China, email@example.com