Original Research ARTICLE
Akkermansia muciniphila-derived extracellular vesicles as a mucosal delivery vector for amelioration of obesity in mice
- 1Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, Iran
- 2Microbiology Research Center, Pasteur Institute of Iran, Iran
- 3Shiraz University, Iran
- 4Pasteur Institute of Iran (PII), Iran
- 5Department of Biochemistry, Pasteur Institute of Iran, Iran
Recent evidence suggests that probiotics can restore the mucosal barrier integrity, ameliorate inflammation, and promote homeostasis required for metabolism in obesity by affecting the gut microbiota composition. In this study, we investigated the effect of Akkermansia muciniphila and its extracellular vesicles (EVs) on obesity-related genes in microarray datasets and evaluated the cell line and C57BL/6 mice by conducting RT-PCR and ELISA assays. A. muciniphila-derived EVs caused a more significant loss in body and fat weight of high-fat diet (HFD)-fed mice, compared with the bacterium itself. Moreover, treatment with A. muciniphila and EVs had significant effects on lipid metabolism and expression of inflammatory markers in adipose tissues. Both treatments improved the intestinal barrier integrity, inflammation, energy balance, and blood parameters (i.e., lipid profile and glucose level). Our findings showed that A. muciniphila-derived EVs contain various biomolecules, which can have a positive impact on obesity by affecting the involved genes. Also, our results showed that A. muciniphila and its EVs had a significant relationship with intestinal homeostasis, which highlights their positive role in obesity treatment. In conclusion, A. muciniphila-derived EVs can be used as new therapeutic strategies to ameliorate HFD-induced obesity by affecting various mechanisms.
Keywords: Gut Microbiota, Akkermansia muciniphila, EVS, TLRs, tight junction, PPARs, Angplt4, Obesity
Received: 05 Jul 2019;
Accepted: 02 Sep 2019.
Copyright: © 2019 Ashrafian, Shahryari, Behrouzi, Moradi, Keshavarz Azizi rafter, Lari, Hadifar, Yaghobfar, Ahmadi Badi, khatami, Vaziri and Siadat. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Seyed Davar Siadat, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Alborz, Iran, firstname.lastname@example.org