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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02217

Relationships between copper-related proteomes and lifestyles in beta Proteobacteria

Rudy Antoine1, 2, 3,  Alex Rivera-Millot1, 2, 3,  Gauthier Roy1, 2, 3 and  Françoise Jacob-Dubuisson1, 2, 4*
  • 1Centre National de la Recherche Scientifique (CNRS), France
  • 2INSERM U1019 Centre d'Infection et Immunité de Lille (CIIL), France
  • 3Université Lille Nord de France, France
  • 4Université de Lille, France

Copper is an essential transition metal whose redox properties are used for a variety of enzymatic oxido-reductions and in electron transfer chains. It is also toxic to living beings, and therefore its cellular concentration must be strictly controlled. We have performed in silico analyses of the predicted proteomes of more than one hundred species of beta proteobacteria to characterize their copper-related proteomes, including cuproproteins, i.e., proteins with active-site copper ions, copper chaperones and copper-homeostasis systems. Copper-related proteomes represent between 0 % and 1.48 % of the total proteomes of beta proteobacteria. The numbers of cuproproteins are globally proportional to the proteome sizes in all phylogenetic groups and strongly linked to aerobic respiration. In contrast, environmental bacteria have considerably larger proportions of copper-homeostasis systems than the other groups of bacteria, irrespective of their proteome sizes. Evolution towards commensalism, obligate, host-restricted pathogenesis or symbiosis is globally reflected in the loss of copper-homeostasis systems. In endosymbionts, defense systems and copper chaperones have disappeared, whereas residual cuproenzymes are electron transfer proteins for aerobic respiration. Lifestyle is thus a major determinant of the size and composition of the copper-related proteome, and it is particularly reflected in systems involved in copper homeostasis. Analyses of the copper-related proteomes of a number of species belonging to the Burkholderia, Bordetella and Neisseria genera indicates that commensals are in the process of shedding their copper-homeostasis systems and chaperones to greater extents yet than pathogens.

Keywords: copper homeostasis, metalloproteome, In silico analyses, beta proteobacteria, lifestyles

Received: 04 Jun 2019; Accepted: 11 Sep 2019.

Copyright: © 2019 Antoine, Rivera-Millot, Roy and Jacob-Dubuisson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Françoise Jacob-Dubuisson, Centre National de la Recherche Scientifique (CNRS), Paris, 75794, Île-de-France, France, francoise.jacob@ibl.cnrs.fr