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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02363

High oxygen treatments enhance the contents of phenolic compound and ganoderic acid, antioxidant and DNA damage protective activities of Ganoderma lingzhi fruiting body

Qingying Dong1, Yeuyue Li1, Gaoqiang Liu2, Zhiwei Zhang1,  Hailong Yang1* and Huabin Zhou1
  • 1Wenzhou University, China
  • 2Central South University Forestry and Technology, China

Ganoderma lingzhi is a famous medicinal mushroom used as Chinese medicine or functional food, and has been accepted across the globe. It is important to enhance the contents of bioactive compounds which in turn improve the quality and biological activity of G. lingzhi fruiting body. In this work, freshly harvested G. lingzhi fruiting bodies were treated continuously with air or with 60 % and 80 % oxygen for 6 d. Samples were collected and determined initially and at 1-day interval during treatment. A high total ganoderic acid content of 29.44 g kg-1 was obtained in samples treated by 60 % oxygen at day 3. qRT-PCR and HPLC analysis showed that the expression levels of hydroxymethylglutaryl-CoA synthase, squalene synthase and oxidosqualene cyclase genes were substantially increased, resulting in the increase of ganoderic acid A, B, C2, and ganoderenic acid B. The scavenging activities with 1,1-diphenyl-2-picrylhydrazyl radical, 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical, hydroxyl radical and superoxide radical and DNA damage protective activity were also enhanced by high oxygen treatment. The results of this work provided a potential method to enhance the active metabolites synthesis in the fruiting body of G. lingzhi.

Keywords: Ganoderma lucidum, Ganoderic acid, Free radical scavenging, DNA damage protection, High oxygen

Received: 23 Jul 2019; Accepted: 30 Sep 2019.

Copyright: © 2019 Dong, Li, Liu, Zhang, Yang and Zhou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Mx. Hailong Yang, Wenzhou University, Wenzhou, 325035, Zhejiang Province, China, yanghl999@yahoo.com