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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02393

Transcriptomic adjustments of Staphylococcus aureus COL (MRSA) forming biofilms under acidic and alkaline conditions

  • 1University of Hull, United Kingdom
  • 2Department of Environmental and Natural Resources Management, School of Engineering, University of Patras, Greece
  • 3Division of Genetics and Biotechnology, Department of Biology, School of Science, National and Kapodistrian University of Athens, Greece

Methicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. In this study, we show that the Staphylococcus aureus COL (MRSA) strain, with a known complete genome, can easily survive and grow under acidic and alkaline conditions (pH 5 and pH9, respectively), both planktonically and as a biofilm. Α microarray-based analysis of both planktonic and biofilm cells was performed under acidic and alkaline conditions showing that several genes are up- or down-regulated under different environmental conditions and growth modes. These genes were coding for transcription regulators, ion transporters, cell wall biosynthetic enzymes, autolytic enzymes, adhesion proteins and antibiotic resistance factors, most of which are associated with biofilm formation. These results will facilitate a better understanding of the physiological adjustments occurring in biofilm-associated S. aureus COL cells growing in acidic or alkaline environments, which will enable the development of new efficient treatment or disinfection strategies.

Keywords: MRSA, biofilm (BF), Alkaline, acidic, Microarray, transcription factors (TFs)

Received: 18 Jul 2019; Accepted: 02 Oct 2019.

Copyright: © 2019 Efthimiou, Tsiamis, Typas and Pappas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Georgios Efthimiou, University of Hull, Hull, United Kingdom, g.efthimiou@hull.ac.uk