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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02433

Emergence of genetic diversity and multi-drug resistant Campylobacter jejuni from wild birds in Beijing, China

Juan Du1, Jing Luo1,  Chengmin Wang2,  Meng Li1, Bojun Wang3, Bo Wang1, Hang Chang1, Jianwei Ji3,  Keya Sen4 and  Hongxuan He1*
  • 1Institute of Zoology, Chinese Academy of Sciences, China
  • 2Guangdong Institute of Applied Biological Resources, China
  • 3Beijing Municipal Bureau of Landscape and Forestry, China
  • 4University of Washington Bothell, United States

Campylobacter jejuni (C. jejuni) is considered as an opportunistic zoonotic pathogen that may cause gastroenteritis in humans and other animals. Wild birds may be as potential vectors of C. jejuni around urban and suburban areas. Here, 520 samples were collected from 33 wild bird species in urban and suburban areas, Beijing. In total fifty-seven C. jejuni were isolated from 7 species. It was found that Nineteen (33.33%, 19/57) isolates were resistant to at least one of 11 antibiotics, especially streptomycin (36.84%) and four isolates resistant to all. Nineteen (33.33%, 19/57) isolates were multi-drug resistance. Multilocus sequence typing (MLST) analysis of the isolates showed that 36 different sequence types (STs) belonged to 4 Clonal complexes and unassigned. Twenty STs (55.56 %) and six alleles among them were first detected. Virulence genes including flaA, cadF, and the cytolethal distending toxin (CDT) gene cluster, were detected in all isolates, but truncated cdt gene clusters only detected in the isolates from the crow, daurian jackdaw and silver pheasant. In conclusion, it was the first detection of C. jejuni involved truncated cdt gene clusters from the silver pheasant. These wild birds around urban and suburban areas may pose potential public health problems as reservoir vectors of Campylobacter jejuni.

Keywords: emergence, CDT gene cluster, mlst, multi- resistance, Campylobacter jejuni, wild birds

Received: 26 Feb 2019; Accepted: 09 Oct 2019.

Copyright: © 2019 Du, Luo, Wang, Li, Wang, Wang, Chang, Ji, Sen and He. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Hongxuan He, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, Beijing Municipality, China, hehx@ioz.ac.cn