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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02580

A novel nitrite-base aerobic denitrifying bacterium Acinetobacter sp. YT03 and its transcriptome analysis

Bin Li1,  Ran Lv1*, Ying Xiao1, Wei Hu1*, Yuliang Mai1, Jingwen Zhang1, Lan Lin1 and Xiaoyong Hu1
  • 1Guangdong Research Institute of Petrochemical and Fine Chemical Engineering, China

Nitrite in water environment is very harmful to human and aquatic animals. A novel aerobic denitrifying bacterium able to utilize NO2--N as the only nitrogen source was isolated for the purpose of removing nitrite from water, which was identified as Acinetobacter sp. and named to be YT03. The growth and denitrification activity of strain YT03 were assessed comprehensively. Results showed that the nitrite in water with an initial concentration of 10 mg·L-1 could be completely removed within 6 h by strain YT03, and the optimal conditions for strain YT03 to remove nitrite were as follows: sodium succinate as the carbon source, C/N ratio of 16, pH of 6.5, temperature of 30°C, and shaking speed of 250 rpm. An RNA-Seq transcriptome analysis was used to find genes that associated with nitrite removal. Compared with the removal of ammonia nitrogen, 47 genes were significantly differentially expressed, including 20 up-regulated and 27 down-regulated genes, mainly involved in the transport process, biosynthetic process and so on. And among the differentially expressed genes, C4-dicarboxylate transporter (DctA) and nitrate/nitrite transporter (Nrt) might be of importance for the efficient utilization of carbon and nitrogen sources in aerobic nitrite denitrification with sodium succinate by strain YT03.

Keywords: aerobic denitrification, Nitrite removal, Acinetobacter sp., transcriptome analysis, Differentially expressed gene

Received: 09 Jul 2019; Accepted: 24 Oct 2019.

Copyright: © 2019 Li, Lv, Xiao, Hu, Mai, Zhang, Lin and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Ran Lv, Guangdong Research Institute of Petrochemical and Fine Chemical Engineering, Guangzhou, Guangdong Province, China, lvran@gdcri.com
Mx. Wei Hu, Guangdong Research Institute of Petrochemical and Fine Chemical Engineering, Guangzhou, Guangdong Province, China, gracehz@126.com