Corrigendum: Emergence of Getah Virus Infection in Horse with Fever in China, 2018
- 1South China Agricultural University, China
Corrigendum on: Lu G, Ou J, Ji J, Ren Z, Hu X, Wang C and Li S (2019)Emergence of Getah Virus Infection in Horse With Fever in China, 2018. Front. Microbiol. 10:1416. doi: 10.3389/fmicb.2019In the original article, there was an error. The GenBank number of the Getah virus strain GZ201808 should be MK487997, but not HM90967.A correction has been made to Results, Detecting GETV in a Racehorse With Fever, Paragraph 1:To acquire the genome of GZ201808, a gap-filling PCR strategy was used based on 14 primer pairs covering the genome of all of the published GETV strains in the NCBI database (Table 2). After sequencing and assembly, the 11421 nt near-complete genome of the first GETV strain detected in horses in China was obtained, including a 66 nt partial 5'UTR, 7404 nt nonstructural polyprotein coding region, 44 nt 26S junction region (ATGCAGGATTACACTACATCTAAAGACCACGTATTACAGACACC), 3762 nt structural polyprotein coding region, and 145 nt partial 3'UTR. The genome of GZ201808 has been summited in the GenBank database with accession number MK487997. Consistent with a previous description, the nonstructural polyprotein coding region of GZ201808 covers the 5'-terminal two-thirds of the viral genome (Strauss and Strauss, 1994). As determined from alignment with other GETV strains, the genome of GZ201808 encodes putative nsP1, nsP2, nsP3, nsP4, C, E3, E2, 6K, and E1 viral proteins consisting of 534,798,523,611,267,64,422,61, and 438 amino acids, respectively. Among them, nsP4 is translated via readthrough of a leaky opal stop codon, UGA (Firth et al., 2011). In addition, the nucleotide length and sequence of the 26S junction region of GZ201808 were identical to those in other horse-derived GETV strains, with the exception of nucleotide substitution C→T at the last nucleotide position in some strains. The G+C content of the genome of GZ201808 (52.50%) was similar to that of other GETV strains detected in mosquitoes, horses, pigs, cattle, and foxes worldwide (52.39%-52.78%). Compared with other GETV strains (Table 3), a total of 12 unique nucleotide substitutions were observed in the genome of GZ201808: G1461A, C1632T, G3291A, T3585C, G3985C, C4530T, C5037T, A5703G, C6669T, C8375T, G8972A, and A/C9494T (numbered according to GETV strain M1), causing four unique amino acid substitutions in the nsP1 (M461I), nsP2 (A746S, D769H), and C (Q75K) viral proteins, respectively. The genome sequence of GZ201808 had the most nucleotide substitutions (nos.:423) with YN12031, which was detected in mosquitoes in China in 2012, and the least nucleotide substitutions (nos.: 34) with AH9192, which was detected in pigs in China in 2017.
Keywords: Getah virus, Horses, China, phylogenetic analysis, genetic identity
Received: 24 Oct 2019;
Accepted: 25 Oct 2019.
Copyright: © 2019 Lu, Ou, Ji, Ren, Hu, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Mx. Gang Lu, South China Agricultural University, Guangzhou, China, firstname.lastname@example.org