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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02625

Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat

  • 1Rothamsted Research (BBSRC), United Kingdom
  • 2Pir Mehr Ali Shah Arid Agriculture University, Pakistan
  • 3Embrapa Meio Ambiente, Brazil

Microbial community ecology studies have traditionally utilised culture-based methodologies, though the advent of next generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture dependent and independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture independent based method. Although culture independent methods provide a higher resolution of analysis, it was found that in the rhizosphere, particular OTUs in the culture dependent fraction were absent from the culture independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant-microbe interactions for the development of novel microbial inoculants.

Keywords: wheat, microbiome, rhizosphere, endosphere, seed, embryo

Received: 16 Aug 2019; Accepted: 29 Oct 2019.

Copyright: © 2019 Mauchline, Nessner Kavamura, Robinson, Hayat, Clark, Hughes, Rossmann, Hirsch and Mendes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Tim H. Mauchline, Rothamsted Research (BBSRC), Harpenden, United Kingdom, tim.mauchline@rothamsted.ac.uk