Impact Factor 4.259 | CiteScore 4.30
More on impact ›

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Microbiol. | doi: 10.3389/fmicb.2019.02693

Regrowth of microcosm biofilms on titanium surfaces after various antimicrobial treatments.

  • 1West China School of Stomatology, Sichuan University, China
  • 2Academic Centre for Dentistry Amsterdam (ACTA), VU University Amsterdam, Netherlands

Objectives: Our aim of this work was to investigate the regrowth of implant-related biofilms after various antimicrobial treatments in vitro.
Methods: Saliva-derived microcosm biofilms were grown on titanium discs in an active attachment model. Treatments including hydrogen peroxide (HP), citric acid (CA), chlorhexidine (CHX) and distilled water (control), at different concentrations, were applied to 2-day biofilms for 1 or 5 min. The viability, lactic acid production, and composition of the biofilms were followed for 3 days. The biofilm composition was analyzed by 16S rDNA amplicon sequencing.
Results: The short treatments of CA, CHX, and HP resulted in a 2−3 log reduction in biofilm viability and lactic acid production immediately. However, both parameters returned to the pre-treatment level within 2 days due to biofilm regrowth. The alpha diversity of the regrown biofilms in antimicrobial-treated groups tended to decrease, whereas the diversity of those in the water-treated group increased. The composition of the regrown biofilms altered compared to those before treatments. Streptococcus and Enterobacteriaceae were enriched in the regrown biofilms.
Conclusions: Although the antimicrobial treatments were efficient, the multi-species biofilms were indeed able to regrow within 2 days. The regrown-biofilms display an altered microbial diversity and composition, which in the oral cavity may lead to an aggressive infection.

Keywords: Peri-Implantitis, antimicrobials, Biofilm regrowth, 16S rDNA, microcosm, oral microbiome

Received: 10 Aug 2019; Accepted: 06 Nov 2019.

Copyright: © 2019 Han, jiang, Brandt, yang, chen, Buijs, Crielaard, Cheng and Deng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mx. Lei Cheng, West China School of Stomatology, Sichuan University, Chengdu, China, chengleidentist@163.com
Dr. Dongmei Deng, Academic Centre for Dentistry Amsterdam (ACTA), VU University Amsterdam, Amsterdam, 1081, Netherlands, d.deng@acta.nl